Quantitative Studies on Antibody Purification: I. The Dissociation of Precipitates Formed by Pneumococcus Specific Polysaccharides and Homologous Antibodies
Contributor(s):
Heidelberger, Michael
Kendall, Forrest E.
Journal of Experimental Medicine
Publication:
Rockefeller Institute for Medical Research, August 1936
Throughout the early 1930s, Heidelberger, Forrest Kendall, and Elvin Kabat continued their work to elucidate the exact chemical structure of antibodies, and namely to determine whether antibodies were modified serum globulins (a class of proteins in the clear liquid portion of the blood that separates out upon coagulation). In this article, Heidelberger and Kendall detail their technique for isolating pure antibodies by using varying concentrations and types of salts to break apart the antibody-antigen precipitates produced by the precipitin reaction. Once they had isolated pure antibodies and examined their nitrogen content and other chemical properties, they concluded that they were, in fact, a type of globulin, a group of proteins not soluble in water but soluble in salt solutions.
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