6th March, 1990. Dear Dr. Nirenberg, I have now formal approval of the Indo-US project. If it is convenient to you may I come to your lab. from 14th May to 16th June, 1990, for about one month. I had written to American Type Culture Collection for my participation in a 5 day programme on "Recombinant DNA -Techniques and Applications" from June 4-8, 1990. If it is all-right with you, I will spend five days (4th to 8th June, 1990) at ATCC in the said workshop. In your lab, it is possible I would like to purify the growth factor from the C6 glioma conditioned medium. You may remember that this factor was shown to stimulate the growth of murine hybridomas. If we could grow the C6 cells in the synthetic medium (devoid of FCS) then it will be very easy to purify this factor. If this is a new factor, then we can do the cDNA cloning for this. Looking forward to come to the Lab at NIH if it is convenient to you. With kind regards to you and Perola, Yours sincerely, (Shail K. Sharma)