N T. Amano

r 4
Cell Designation... 1. Je.eeee

 

 

Ampole designation... Geeees | Box Bojer eee. . Total # of vials
date of freezing..12..29761.-: | Passage Hove Frees weeee
7 | |
1) date of primary culture | |
“ : ! 7 | ' I
2) origin of cells ! !
\ { i : : : .
3) date of last subculture | cell # per flask ml of media! type of flsk
/ | ! : ! | :
Media itr (671025 ) Horse serum ; Fes (¢ 1s p dB sg /6_ ml
7 : C3) | !
date of freezing | {co % confluent color of media © |
Trypsin used ( sol # — |) ~ % in media....... | sol.# ) i
¥ i FES A i , | oe ,
Centrifugation | foeerpe. fo | '
Suspended in. — {.O. ml of Media ( iy
Freezing media media i % of serum [ [oh DMSO ( lot# )
Fite. AHS of | |
oa af |
; : '
argoules: _ type bmxX/o approximate number of cells/ml/vial
| ak. :
, ; ne te ce ee aoe - Loe Le te - i —_
Freezin g method | | |
woe ee r ee ee ee pov tte eee ee *.
4c i [eee hours) a.m. ; i a a.m.
o : | P.m p.m.
e200 f AG hours | asm. pom. \- _ a.m., pom
-170 C pO bees .. hours 2M) Dem 4 a.m, p.m
, | : Pd | |
; Peak _ | .
Remarks; | 4
, ‘ . | |

 

‘ : 1
Numbe =~ of vials taken......; we DYsccccdeccects GMOs cceeccebevecseceee |
Cells In Freezer
7) Cell designation... VAG becececeeeees
2)date of primary culture
) origin of COLLIS. sesceeseesoous eeee
2) date of the last subculture. .i/%Vecscres
5) date of last medium CNANZGcccccsccccecece

6) Media Fiz % lot# ( o4te2s )

j2f3e [64

Date.crcscece

ES lot#
FCS % lot#
cell morphology
€ confluency
pH color
7) Trypsin used Oe | ~ in. Rt lot# MAGC.cccccscceccece
BOls#ecccccscccecces
8) Centrifugation owe rpm of min.
. > (© @ pxso
9) suspended fe in media Fi % lot Lot#esveccoee
HS YY  S £ lot#
FCS c # lot# % Glycerin
lot eeeeeeoeaeee
cell count /al. total..../,? Co 7

11) Freezing method eoeoe M1. Of ampoules
a
d. freezing rate C/fmin. 12) h ampoule #
/ “eq aw eecveceels
PeMe
/ -20 C a.m eovvceeehle
Pelle
o
/ -170 Cc ae. eoecvceeNhs
Pem.
Remarks:

YE 13) Box#ecesccbeccccceeee

date of steril
autoclaved,

in freezer or

in freezer

in freezer