Table XI. The frequency of positive sugar fermentation markers appearing in the tested recombinant colonies of the different recombination methods. NUMBER OF POSITIVE MARKERS METHOD Maltose lactose arabinose mannitol xylose Colony "L form" 66 58 50 17 16 Normal 72 64 52 18 10 Standard Non-centrifuged 100 89 68 17 12 Centrifuged 75 62 56 13 12 "Protoplast" Non-centrifuged 4 3 4 1 1 Centrifuged 1 1 1 0 0 TOTAL 318 277 231 66 51