aN

Lister Institute wf, Chelsea Bridge Rd.S.W.1
Dear Josh, | |
This is just. a hurried request for some information, which is

possibley in the literature, but if so I can't find it. I have been going

ower a paper of Clowes and Rowley which they have sent to the J G Microbiol,

on the genetics of nor~leucine sensitivity in K12; they find the lecus for her Leng
resistance tops closely linked with the Gal ~ and Arab- loci of ¥ 677 and
less so with some of the other sugar markers of that strain. AS a bye ~
product of their crosses they also got linkage data for the 6al and Arab
loci and the nutritional markers, and these of course do not agree with the

data for the same sugars in W 945 given by Cavalli and you in the PF faétor

‘papery they have ascertained from C that the Gal loci concerned are known

to be non ~allelic, Presumably the arab loci Concerned are also non-allelic.

They have explained this in a rather laboured way in their paper; I have —

suggested that it would make it much clearer if they could give the correct.

ag 1 Ovi: hrehViprcyt

‘subscript numbers to the loci. However t Gant find what they should be. ¥ 746

ie in Ebs Lac paper as Gal 2 3 is this the same as W945? I cant find v67785
Court forioth Ainley tir; betercet Gry Irma ?
marker's listed. I told them that as they were. both presumabley your strains
originally( since they have W numbers) , the best thing would be ask you for
the official loous numbers which you have assigned , and I offered to write
and ask you . Is Gal4 the only one near lambda? If the Gal- of W677 is near
Gal4 the norleucine character might be usefull in E’s experiments.
Nothing new here . I have just given a broadcast on Pneumo transformation

in a programme they have called Science wurve-y 3 I said firmly that DNA
molecules were genes( no time to be guarded) and shall now be interested to

see if any real geneticists write in to object. I missed the moet interesting

bart of the actual broadcast (they recorded me the day before). which was

ee acne veqvane nese dimunge TET Cacwawenenaneneneannanmese es
the, un aver i
Mean) that I oringly Mae how Co
4 be to haby hel wo chirig v0", My eyyetogce a cut of lhe ” olen - Sooe tae Page,

they accidentally began announcing it 7 minutes early, in the middle of

 
  
 

reaction to go say if it is betweenPR~of 553 and Hi, or on the other side s
of Hl, beyond SL2 Fa,. ¢

fatel ml pith fro gor tn Je ow ra 8, Goa

—Whinek
Ope ne
Arey botre f 5
ake Luial tbo Lape Prceme 5 gore, O
L oe pecrfrlipivetwiel BoE mb

foo o9 I ca Qa GE Wy
bb one. bin Lo nes 2 yy Cee, MA ore cloaeine,

CLAN

ane vet ww wey fo afl as
J asoned eT Cone ap oe M1 gem ane MWe fso restieks, 7
Nittany Ow hits a jb [5 Lou Cnfugien we Ha Cb owton June nv

~~ First fold here ———»

Pe

music. Ss
Fla - cus of S966 is definitly Hl linked we still nee@ one more ub
3