May 30, 1953
Dear Bruce:
Just a brief dnterim note.

S. gallinarum (CDC~74) —-x SW-1040 (IX XII a:— from sendai—xH901)
gave gm:-—-. SW-970 and 972 may have been true bills as "aherrant galladsarum",
7 other strains of gallhfdarwum also gave g..., not yet fully types. Nothing
from pullorum, though I am still convinced about tracks and swarms —x 0901.

SW-1048 (from your SL-13) does seam to be more readily trans(in)duced.
Swarms have now been obtained in

SW666 —x SW967 (gp) I think there may be some circumstantial
967 —-x SW666 (BE evidence for the close linkage of these three
967 ——x SW1O48 (a) Fla- factors, but it is not at all conclusive.
bd 12,7
S. paratyphi B java (N25 and N97) seem to be likely H HL?" HH, -

Hy “H, ° » and similar combinations have been obtained by the two step importation

of these first—phase antigens; the rate of phase variation is very low and
somewhat erratic. I doubt that the 1,2 phases can be mtations from the h, as
previously thought. Ho can be transduced to a:c. Unfortunately, the critical

result, a triphasic atcsenx, has not yet materialized, perhaps only for
technical reasons, and the very sluggish rate of a:ce in the parent anyhow.

Anderson visited us during last week-—— we had a good time with him. He quoted
a story that you are possibly moving to the Lister. If so, the very best luck
to you. Talking over the ms. with him, a few points came up, which I present
as my own, not his.

In the text, aside from references, "Felix" "Taylor" ete. should be prefixed
Dr.(A) or (Joan}.... The firstnamed especially might be rather stiff about it,
Anderson's name should certainly be adjoined to Felix' in the acknowledfZments;

I gather he was directly responsible for doing the lab. tests, for arousing
interest in having them done, and for communicating the preliminary results to
youninformally. Similarly, I would add Spicer to Benstead on the serums.

The following comments are of varying importance. I think that might add to
clarity.

pel6 23-24. ESA could not be sure whether this implied that gall hharum had also
been transmotilized.

pe2l on "foreignaess"—— this remains a difficult section. Logically, it is not
excluded, actually, that "foreignness" is a sufficient, if not a necessaryp
condition for trahsmotilization. I.E., sither "foreign" phage or Fla+ homologous
phage might function. But more to the point, I think lines 6-14 could be deleted
as repetitive of the previous argument (and as carrying the awkward construction
“foreignness"), and that you can proceed directly to the facts after"However,
phage grown on a spontaneops motile reversion from the }-strain, SW-534, evoked
numeorus early swarms from SW-534. Also, a swarm.... (this last secttion is also
particularly wordy, dvey in repeating "this motile derivative of SL-15", as
could be averted by a simple recasting, but I don't want to go into such details
now). At any rate, I suggest that "foreignness" be either deleted or replaced
by an adjectival construction with"fSreign". On the whole since the evidence
is not decisive a brief summary ststement that phage from spontaneous reversion:
There is, in fact, an extensive series of experiments along these lines, referred

to for other purposes on p.28. SW666 —x SW666 gives, of course, nothing.

SW666 (spont. Flat) [ 2 different strains] —x SW666 is effective, as were sevexal—
motile transductions £-xSW666) —x SW666. The SW666 case is especially convincing,

in view of the very low rate of the spon&éanous revewsion. But even with SW534, it

is scareely necessary to repeat the walification(on p.9, 10 and table 2.) on spontaneous
late swzzms.

20-19 (not to cancel previous comment) B ¥4i for Vi B
2la (Begins Flagellar Antigenic Phase Latent in cells....)-15 lysates
~20 often mtates (not always)
7-11 "phage treated area"—— this does not sound so smooth. — try? area exposed to phage.
9-6 commas are confusing. I think they are needed after strains,and after agar, to
set off the qualifying clause. I can think of a better, more drastic reconstreution:

The growth of most of the non-motile straing wae—-, without pha ‘exposure. to phage >
strictly < On semisolid agar
was confined to the site of incculationy even after 48 hours or longer .Anoubatien—at-—

39° (Fig. 1) [Conditions of incubation already specified, p. 8].( One can arsue for
keeping “without phage treatment"; I would regard the title, just previous, as a suf-
ficient reminder that the experiment is just as described). What I am afraid of, hers
and gelsewhere, is that too mayrqualifications detract from comunicating the main point.
Later in this paragraph, you refer to induced swarms. This might better be transposed to

the end of peli; actually, 1t is repeated on ps» 14, where a single sharper statement
could be made.

15-- Olitzk
31-10 simultaneous 32-27 however

35 organelle
~20

I hope this irruption does not unsettle you. aAndereon read the paper carefuily while
he visited, and, I may say, quite independently came up with much the same general com
ment as was in my last letter. I am just a little afreid that the luster ef the scienti-
fic content might nvershadow the usual editorial criticiem at the hands of the referee.

In writing now, I am assuming that the me, will have been subritted for publication, but
that you will still have an opportunity to revise before it is sent to the printer (and
may be encouraged to do so by the editor). I am too deeply indebted te you for your
editorial coment on "Cell Genetics..." for me to doubt your own critical judgment (or to
propose my own as preferable), but if my experience with that particular paper is any
precedont, you wlll be so thepoughly tired of the ms. as to wish to be ridcof it. If a
Andy' hadn't come by, I would have had the ¢¢###¥¢ same fecling about it, but the :
comment he made (with which I could not disagree )was rather unsettling). I might add

that he concurred with my argument about species/serotypes. I gather that the Interna-
tional Subcormittee uses the latter advisddly, and as my last letter indicated many

turns of phrase are simplified by type. Have you discussed this with Joan Taylor?

Wo had our first real swmer weather today. It reminds me of a happier time nearly

a year age when the ewb content of our exhilirating argument was substantial rather than
verbal.