March 11, 1953

Dear Bruce:

I have been wanting to write you for some tine, but wa ighibited by the
arrival of a package of cultures, as I have been expecting a letter from
you to follow. Howeverm this has not arrived and I thought it best to let
you know.

Going over your letters, I note occasional requests for cultures-— have
I filled them all? It would help me considerably if you could summarize
and list anything you can use. Esther and I had a very profitable visit
with Edwards, and 1 am still trying to digest some of the material I brought
back. Here are some odd notes, cultures, etc:

1) IX XID 1,2:1,5 from Edwards 157 —x miami SW 973

2) A few more nonmotile typlimuriums, paraB's and groupD's. Ag doing homology
tests, and will report and send as ready. Have you tested homologies of SW553
or are you confining yomself to TM? One of these has given a track fully 21
mn long(in projection} agtually longer) and still going. So far it has been
single, but it may have just branched.

3) SW553 was part of an epidemic: have gotten some other cultures, (came from
Guatemala, calves and pigs)mad am seeking more details. Four svailable cultures
seen so far identical in cross—mnot. tests.

4) Peculiar regults with abortus-equi, Edwards #26. No effects whatever —x 26,
but 26—x SW-666 gave several IV V XII a:--. This may have do do with Edwards'
and Bruner's finding that an a phase can be gotten from ab~equi with great dif-
ficulty, and wkkk would resemble previous findings that TM phases 2 —x 3666
does give i:— as frequently as phase 1. Suggests that SW666 residual genotype
does permit activity or activation of otherwise tnactive lcoc1. On the otherband
26-~—x LT-2 gave an enx:——- which ssems stubbornly monophasic.

5) Other misc. S. zega —x 666: d:--; 35. javiana —-x 666: An inaggl.
that arose in a —x abony exp't proves to be Z, 7enx. Z3 is ‘ orotaely a mutant
allele of b. (As is the 1,2 of #157).

6) A nonmotile regarded by Kauffmann as aberrant gallinarum was not transmotilizable
by var. Fla* FA, but —x 666 gave (gm)+. Am testing —x various NM to see whether
it turns up as demonstrable double. "xtensive tests --x puklorum and gallinarum,
various strains, gave no motility, not did I get Mal+ from pullorum. (Am planning
to try pullorum Mal+ variant —x pullorum). Also pullorum, gall,—x 666 gave no
motility of Gal+. I suspect rather more than gost adaptation.

7) Edwards fully sympathetic to JT's serologisal work being presented. He
has donemet quite a number of other transductions, mostly diphasics, and will
not have duplicated work you already mentioned on —x666. He is in fairly
close touch directly with JT.

8) How is paper? 9)What are these last strains. 10) A spec.
lambda stock turned up, transductions:plaques more than 1:1000. Isolated trans
sductions appear to segregate regularly. 11) Some peculiar stuff on formalin
~labile H antigens in cholerae-suis. Not too relevant. Cf. Spicer.

My apologies for so many fragments. Will communicate more fully after I have
some time, and have heard from you.