LonG ISLAND BIOLOGICAL ASSOCIATION
COLD SPRING HARBOR, NEW YORK

THE BIOLOGICAL LABORATORY

November 29, 1954
Dear Josh,

I have made a few comments and rewritten one section (insert I). However, I
don't feel very strongly about these points and wil] be willing to let you decide
which changes are worth making. I do feel that a little more detail on the method
would be helpful -perhaps not in the form I suggest. J ama little confused myself.
Almost invariably, when working with NGA plates, I transferred daily so that I have
little idea what would happen if the pkakaxwexa first plate had been allowed to
incubate indefinitely. My feeling is that it would have dried up badly before any
good swarms had a chance to form. Weren't most of your motilizations if tubes? Or
did you prevent evanoraticn better than I?

Comment A. ped. Altho both components in each mixture were quite motile and
the objection is minimized, it's still true (as you imply) that only a few of the
inoculated cells may contribute to the swarm periphery. -So that the results could
be fortuitous (from 1 exnt). Couldn't something of the following nature be said:
"Qualitatively similar results were obtained in repetitions of this experiment;
in each instance, the final Gal-/Galf ratio was kixkexxkhamxthe markedly higher
in the Galf Ff x Gal~ F- mixture than in the mare involving the reverse coupling.”

(over
The experiments are very nice; altho, ofcourse, a little disappointing.

If you or Alan make no additional major changes (of your MS) I don't think
it will be necessary for you to send it to me again, if this would be especially
inconvenient.

Rumor has it that you are going to Australia?

Best regards to everyone.