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vouparative motility data on WG lines

 

The accompanying table summarizes details of a survey of lines
1-31 for presence and vigor of wotility. Column headings give time
elapsed between tineof reading and time of inoculation, plus type and
quantity of culture. Those not designated metag were pennassay broth
cultures at 37 0. Those designated metgag were swarm tests on petris
filled with idward's wotag. A411 constitute independent subcultures from
refrigerator stock sdants, with the exception of the two columns headed
by an arrow andthe word "then". The second column here was a direct

subculture from the immediately precedinp one.

Broth cultures were examined microscopically as follows: «# small
drop, ca. 0.05 cc, wags hung on a cover-slip over a depression slide and
scanned quickly under high power. If no motility cald be observed,

observation wae more prolonged. If motility was observed, percent of
cells moving was estimated. Erownian movement was usually easily
distinguishable form true rotility. Since vigor of motion varies, it

is possible that the figures given are more appropriately estimates of
vigor, rather than estimates of percent motile cells.

Tnformation on motility involving no quantitative or pseudo-quantitative
data is noted to the rizht.
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