en ek cr eae ee ial

ON ae ORE OREN ar

i ne

UNIVERSIDAD DE CHILE

_ESCUELA DE MEDICINA
INSTITUTO DE QUIMICA
FISIOLOGICA Y PATOLOGICA ; January 3, 1958
Borgefio 1470 - Santiago a

Dear Josh:

I was waiting for you to get back to Madison and
settle down a bit before answering your letter of October 26.
L guess, by now, you must have fallen back in the rout ing,
Haventt you?

You must have heard about the loss of the original
ms of mine, The editor wrote me asking for it and I told Bette to
send it thinking that she must have had it. Probably I was right
because I got a letter from Bernard Davis saying that he was one of
the reviewers. .

Bernie wag kind enough to send me a copy of his
comments. Most of them are justified and I kmew them from the
very beginning. | The most important is the mixture of experiments
eiven in the paper which makes very hard to understand the whole
picture. Bernie suggested that it should be broken dowm into two
papers. Another point, also very justified, ia the anacroniam of -
the paper in the sense that Monod's permease only appears at the-
end. With regard to this point it is very hard to find what to

do, because, in fact, all the experinents and conclusions were
prior to Monod's permease.

Itli wit for the official word of the Editor to
decide about the ms.

With regard to your owm comments on the ms I have taken
note of them and I will make the corrections when I will have the
occasion. *

‘In connection with reprints, I will give the
instruccions to the secretary of the Jnzyme Institute when I have
the forms from the J, Bact.

I will write you in a near future about some experiments
on the permease problem. I have synthesized Cw1l4 labeled THG but it
resulted of a very low specific radioactivity, therefore the time
required for the axperiments has gone up considerambly on account
of the number of cells necessary, new technical problems, etc.

I have decided to visit tho States next August. I
feel urgently the need’ to talk with people of the field. Specially
now with so many interesting findings (which I receive six months

later).
I was rather discouraged to read two papers on labeled

DNA, because I think that [am to much of a perfeccionist. Both

authors worked with a DNA dar more impure than the one I have considerec
unguitable(o.1 % of low molecular weight nucleotides), A student is
coming next month to work with me and I plan to put him back in the
problem but using thymine-Cl4 to label the DNi. Do you have a thymine
 

 

heue rhery = puge &

requiring mutant of KeL2? By the way, the two strains 2242 and 2244 vere
Tocolveds thank yous ;

Please write about the news in Madisom, scientific and others.
My cougin, Sara Windefman, is allergic to saabehaaladl so I never hear anything
sah anybody there.
With my best regards to Esther and you,

yourd,

  

ee en ee 2 i"
" “oe tke ee Pome ee ake Beem oe ae