INSTITUTO DE QUIMICA # 3

FISIOLOGICA Y PATOLOGICA
ESCUELA DE MEDICINA
UNIVERSIDAD DE CHILE

Borgofio 1470 - Santiago
=a

September 11, 1956

Dear Joshs
I just received your letter of August 31 and the
copy of the letter to mr. Berger.

As I wrote you in my last letter, it seems that the
price of ONPG will be still $ 20.- per gram. I will wrtte to Mann
today asking him to give you mspecific instructions with regard
to your position. You can forward him any orders which might
arrive in the meantime.

. Thank you for handling the orders for Bernie and
Dexter, we will take care of your "commission" as soon as we
receive the payments. With regard to the order of Bernie we
received already the check, should I send him an invoice?, or
you did send one already?
You can write Mr, Berger that for amounts over

10 grams we can consider synthesizing the ONP-glucosaminoside
and the ONP-N-acetylglucosaminoside, assuming that the synthesis
of these compounds has been studied. He does not state if he

\ refers to the aor 8 forms. The price will range between $ 30.-
and $ 70.- per gram according to the labor involved.

I believe that the man from DuPont was Mr. A. L.
Foster, unless he is the agent. I have instructed Mann to fill
his order as soon as possible because I received a telegram from
him long time ago.

I believe these takes care of the busimness part.

I am very interested in Monod's manuscript, I hope
Sol sends it to me soon. I am stuck on the discussion of the ms
on activation, if the inspiration doesn't come I will send it to
you as is. I found always your criticisms most helpful in spots
like this.

IZ was surpris&dg to hear that you are going on with
the propagation of propoplasts as L-forms. Last time I heard you,
you did not want to study further the protoplasts. What made this
change?

If my strains do not arrive soon, I will have to ask
you for new cultures, In the meantime I got a phase microscope,
and a De Fonbrun micromanipulator. We prepared also a very good
looking DNA from protoplasts of W 1317 and we will start with the
experiments very soon. The yields from E, Coli protoplasts semm
to be far better than those from E. Coli as described by Boivin.

ound a reference for an "electric" micromani ulator
which might ££ ound, you(how is your design coming along?):.

Petence,115,646(1952). Bet reqava> fo @bke- a..0f you Sous ~