September 3, 1955

Dr. E. D. Rosenblum
Department of Miorobiology
University of Texas

Dallas 19, Texas

Dear Gene:

I will send you som reprints when we do a regular mailing in a
few weeks.

With Pseydomonas fluorescens, a few yeas# ago I made some auxotréphs,
and diauxotrophs, withba regime of UV, penicillin and replica plating.
These were all prepared from one of Staniesrés strains. They gave no evidence
of crossing after mixed culture. No special problems in making the mtats.

We had hoped to extend the search to testa from phage-mediated trans-
duction, However, none of our own strains appeared to be lysogenic on
any of the authors as indicatora, and Jacob found no cross-reactions
between the strains I had sent him ani his P. aeruginosa phageed 2 one
of my students is interested sometime, I hope to make a more exfensive
search for different phages and different strains, but there 1s nothing
in the widd right now, and until then, I do not plan to make any further
public comment.

Loutit had a lot of trouble getting auxotrophs from aeruginosa, I hope
you have beéter luck.

Yours sincerely,

i f
Soghtid Ataderber g

f
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