RUTGERS UNIVERSITY
The State University of New Jersey

INSTITUTE OF MICROBIOLOGY

NEW BRUNSWICK, NEW JERSEY
CHarter 7-1766

Dear Esther and Josh,

bisiness ;
(I) experiment # 269 was in three parts - unlabelled as to subparts = with
three crosses with diploid isolations:

Was Fae Wirey
W2S7p eR Rtas
wig x * yw ?> é i
(2) concerning Gal 7 of W583 (which I was given with the label Gal a):

ae W 583 and W 2406 were both mixed for Ipo and I selected the one
which was given in the book - both cultures were singled by
me (but these would be derivatives of the master stocks)

be /322/#4d W 206 never tested on EMB galactose since W 583 X
W 2406 gave Gal + recombinants ~

Ce unusual G1 fermentation color of Hts IV-2.and IV-76 is recorded
in my notes but attributed to medium differences (?)

de 'reversions'of Hts IV=2 and IV-76 were never back-crossed as
far as can find in my notes

Thus a Gal=slow in 206 is not ruled out by my data. As noted in my
book 583 was called G,l a because it was not yet sure that it was Gal 7 -
something to do with ability or inability to be transduced,

(3) wrote library re book which should have been returned thru usual
channels ~ I found it the morning I took off for Cal and left it to be
returned but apparently it never was

(4) nothing new here research wise

(5) by Parisian (ieme) informant and from CSH < Jacob has tirrefutable proof?
of partial transmission in Kl2 - as near as Ll can figure what he does is this:
cook cross in buffer or broth/£6¢/tdty1, withdraw aliquots as function of
time, pop into “sring blendor, plate for recombinants and test for battery
of unselected markers ~< findings: longer ane cooks before blending the

tee the frequency of recombs with unselected marker derived from F+
or, rather, Hfr) parent

CSH very excited about this according to Werner Braun = but I pointed out
that this is solely a coitus interruptus experiment and progves nothing about
the normal mechanism except that one can chop up the chromosome with a

Waring blendor - apparently Yvonne L.nni has done the same thing with
phage (using two marked phage and blendor).

other

(I) was at CSH phage grad = Gus Yoermann fell and broke his elbow = had to

have bone chip removed but is recovering uneventfully according to late reports

(2) saw Seymour - he and Susie Mayr quite thick by criterion of squirting

each other with water pistols all day - no discussion of science

(34 rumor that Koger R id of ONR will be next director here _
Bernie Davis turned up with wife at CSH - runor that he left her waiting

at the church also rife - but apparently the wedding did come off
(5) Braun is foaming at mouth here because anti-genetics faction (everyone
but Waclaw, Braun, and myself) maintains:
"nucleus has nothing to do with cell growth, metabolism, etce"
"chromosomes are waste products of cell metablosim - made up of alkaline
phosphatase condensed on unspecific protein and metaphosphate"
"mutants are due to different conditions of nutrition of the céll"

(6) point brought up at Brookhaven:

re Demerec et al linear subgenic structure - only one found for each
amino acid in Salmonella but there are loci scattered over all the
chromosomes in Neurospora = why?
answer by Davis or Hotchkiss - maybe several pathways of synthesis of
amino acids in Neurospora (but then why does a mutation at one point close
down the cell?)
Waclaw, Braun, and. I got to talking - prposed-that amino acid, etce,
is not merely synthesized at a given locus (or structural mechmism of
synthesis determined at that locus) but also incorporated into a
pre-fab sub-unit of cellular structure - thus each locus is
indispensible but externally supplied amino acid, etc, can be
used - to test this idea labelled amino acid would be fed to several
mutants and resultant myelium fractionated for strectural components -
‘celd walls, mitochondria: (?), ratio of labelled to total: #6#4/ amino
acid should vary in different fractions, furthermore, unlabelled
-compenent should be found (but diffieult to test fih¢d¢' obviously)
What do you think of the idea?