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Cn

S22

August 27, 1

Dear Jacques:

Ioan sorry to have taken go long to read Your ASe, bab it arrived
while I was away o: vacation, and I have tended to it at my first op-
portunity. I have just forwarded it to Sol. DRor'ts Retuaa has returned
to Chileaddress bela and T will ask Sol te pass it on to hin.

fo don't have to tell you thas this is an inters stiig and exciting
story. There tc just one experinental fact » however, that I fael ig
lacking for the understanding of the functional relationship of
and Jy vitro ralactostdase amtivity., When JT rac inte tbs alecepen 1ey
umix between intact-cell and extract activity, this aoticon was of
course obyious and pree: uinent, bul Twas puggied by the feilure of
azide to influence tha act ivity of the intact cells, And I still ax.
If the permease is sensitive to azide, and ds necessary for ia vive
activity, why is t> “ere ne effect? Should net an auide~treated susp:
of inl fesed or equstitutive cells behave like wor énduccd ob consti wscs
Leag.y 2 nee taerefare seen to me possible that the yesysten does av

 

  

 

 

inffuenss the pate af aS Ser of substrate tte te sclis, despise ¢
other re reenadcedie pronerties, Or do you have o Atrect measuwe of &
vated wiley T have oy soloviend:

“oo my owa alarification Seo, vou lave mecideued that Lata” i a
eryepleity subant., Be you happen to have any exparinantal fLpures

comparcbie to that of Tuble 7 for the CaLe mrtan: 72 01 nitzint have
missed the effect from using as eoatrels cultures that alrealy had .
A meyhek oryeptielty factor of about 100. Ie is possiiie «: " bac
has a different pe Shore, of change of crypticiuy oi: azine: than docs

  
  

    

  

a

 

ON, semistoable

On p. 18, you refer to the searmleticn, ra
: *, mpm ae we oe
Gos Meteal prool of 163

 

Tee.” oad Lae A 9 UO Fou have dehy i
would hope *hat vou would restore vane misaloy Gawle, ae ft Le verr
diffievit to make a critical appraisal of we wnd0US5L09 of Dunctional

relationships witha “te.

 

   

“her and Gurchesh have boo etodyvire shea ;
oe op nitants, fe you mow, they recomise thres engrantie st S033
1) Gal+ F— Galel-p (sinage); 2)Galn2~" + “Pr — GlawleP 4 1
(transferase); 3) [PGs] — MPC (ueldenase). Sa fer, Gal,: Gel
gel, and Gal» mutants Lael me transferase fas de the : tae Eosemi a
wmtants of sapiors); Gal, laexss the kinase walie

se der (thes ney be blocked 2a a vite sh minthast 3 moo sbose: ve should
loans f or velactose-derendents!), curban’ seme, of sourse, all

very, e sosely dinked, Bev sent nea j Anke 3o Snese, end aot

in ; Taesa Siaedliags
aed eh pre “ininarers we are Lue KEX continue
thei: enaly3es. (Tt is tras

wa positiar
» bUt T hoeiahes 6 ‘ious conclusions itil

nese mitatte

Wowk

     
       
 
 

  
 
  
   

 

. au very wuch for letuiac me read voce us, With best : BNGS,

¥

 

Raytrerrs te a ttdaen mee amen FO ones