National Institute of Genetics
Misima, Sizuoka-ken, Japan
September 30, 1959

br. J. Lederberg
Department of Genetics
Stanford University
Stanford, Cal.

U. S. A.

Dear Josh,

Would you tell me your plan on the manuscrbpt entitled
"Monophasic variants in Salmonella", which you have agreed to write
followed my thesis and the abstract. If your business still have to
keep you off from the manuscript writing, I will undertake it and
willjask your revision: originally it is my duty as 1 am to be the
senior author of the paper. If you hesitate writing because you
think my data are not complete enough to publish, please let me.know
frankly your opinion about it. dim: One reason I write you about the
manuscript is that Dr. Beale asked me to tell him the progress of my
researches on the genetics of flagedlar antigen after 1956's paper
for his review article on "Ayjtigeneic Variation in Unicellular Organisms"
for xm the Annual Review of Microbiology. Another reason is that
I have a plan to start writing a paper on the morphological mutants
of flagella, in which I have to refer the study on the monophasic
strains.

I obtained a P22 sensitive clone from the i:b strain, SW1162
(the origin of which, as you may remember, is, SW&03 brenx —x SW1061
-(i):1.2 -» -(i):b,SW1161. ‘M2 i:1.2 —x SW1161 -» i:b,SW1162 , see
my Research Report (July 6, 1956)), The transduction i:b —-x atenx
produced i*enx and a:b but not xx% a:1.2. enx-antigen type has not eer
detected from the a:b clones so far. Anti-~b..e03 serum and =bey116]
serum were prepared. The cross apgkateuxtsms absorptions do not remain
any agglutinable fraction. I am inclined to assume that the strain
Sw1162 has‘ H,i H>>* rather than it is heterogenote or Hj~-duplicant.
The Ho might have been produced by a kind of unequall recombination:
H, and Hp might be homologous in origin and have an affinity to pair
in rare frequency in case the transductional fragment is small enough.

Last semester, I gave two credits of lectures on "Bacterial
Genetics" at the Graduate School, Tokyo Metropolitan Uniyersity. In
the comming congress of the “enetics Society in Japany will be held
at the beginning of November, I will be a chairman of the "Microbial
genetics" sectitn. Hirota wrote me in his letter that Esther asked
him on my present marital status. 1 am still singe. Please tell
Esther that I am sure I will_write her any progress in the statas
as well as in the BE. coli x galmonella study.

Best regards to Esther, Ann, and Hirota san.

Sincerely yours
Zia
f- 5 Y haewe ano Kew Fo —y niwv tout
7h> Brant fre nm N/a grt.

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