Guiversitp

CARLTON, N.3,

of Melbourne

VICTORIA, AUSTRALIA

 

Bacteriology Department
BH. HB 5th April, 1960

Professor and Mrs. J. Lederberg,
Department of Genetics,
Stanford University,

Stanford,

California, U.S.A.

Dear Esther and Josh,

Thank you for the information about Barpn's work with Salmonella.
I wrote to him about it but never received a reply. Actually I
have been doing some more experiments to transfer F to Pseudomonas
and FP to Coli without any success. However, it locks as though
this work will be continued by Barbara Fargie and Luca in Pavia.
As you know Barbara is over there and from all accounts doing very
well. Luca is interested in doing some joint Coli-Psewiamonas
experiments and I think the environment of a qualified Coli worker
and a qualified Pseudomonas worker should have a very good chance
of success. I have sent a number of strains of Pseudomonas to
Pavia and I look forward to hearing the results.

David Gray in this department has obtained some interesting
results from cattle infections resulting from more than one species
of Salmonella. This has interested him to the point where he
would like to examine mixed infections and possible genetic recom
binations in vivo, and I am going to assist with some phases of
this project. I am wondering if you could let us have some strains .
of Salmonella Lippe urlun. What I would like is sane Auxotrophs
and some fermentilarkers of LT2 or LT7. It is a little difficult
for me to ask for particular mutants as I do not know what you
have in stock, but I will not need more than two or three auxotrophs
and fermentation mutants of each of these strains, so I shall leave
the actual selection to you,

VJ (Kong yy *//

I have been meaning to send you a copy of the recombination
manuscript which has been accepted for publication by J. Bact. but
at the moment I do not have a spare copy after the one I sent to
Barbara. However, I shall let you have this as soon as possible.
We have two other papers in press, one on the use of lysogenicity
and pyocinogenicity to type Pseudomonas strains and the other a
more general study of Pseudomonas phages. In addition, we have two
more papers on the transduction work in preparation. More recently,
I have had the opportunity of collaboration with a nucleic-acid
biochemist and we are locking at the DNA of Pseudomonas and its phages.

/Bob....
 

oY PYF 1A 2
Bob has left to go to Canberra and the latest report is that he is
busy learning all about these creatures called plants. I think it
should be a very interesting problem indeed and undoubtedly Bob is
working with a very active and stimlating group. Your prognostication
about the building was right and already we are expecting a twelve
months delay in its completion. You,should be seeing Syd in California

some time in July on his way to New York. Frank Gibson is back in fine
form.

With my best wishes,

Sincerely,

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