PUBLIC HEALTH LABORATORY SERVICE
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Central Enteric Reference Laboratory,

GENFRAL~PUBLIC-HEALTH-LABORATORY,-

COLINDALE AVENUE,
Lonpon, N.W.9.

  

Telephone: COLINDALE 7041 (8 lines)
Telegrams :: DEFENDER, NORPHONE, LONDON.

46th July, 1953.

Dee Fock
. I am enclosing herewith a table showing the susceptibilities of Salmonella
strains other than Salm. paratyphi RP to the varatyphoid-3 typing phages. The
phages. were applied in two pools as indicated in the table, each phage in its
routine test dilution, and it may well be that higher concentrations may yield

wider cross reactions. For the sake of completeness Grouos B and D are also
included.

Unfortunately, one of the phages in Pool 2 ("Newburn" ) has now been dis~
carded and is no longer available; 7 hove thet the cross reactions were not
due to this phage. Phage "Jersey" hes been added to the group since the
experinents were done in 1949 and was not therefore included in the cross tests.

The protocol shows a considerable cross reaction in Groups other than 3
and D. Pool 1 is evidently uuch more restricted in its host range than Tool 2.

The cross reactions of the Salm. typhi-murium typing phages were restricted
to Groups B and D, so I have not included the record of tests with these phages.

I am forwarding under separate cover 10ml. of phage k, the Yate of prep-
aration of which is on the bottle. The lysate was freed from the propagating
strain of Salm. typhi by treatment with toluene. Careful sterility tests have
so far failed to demonstrate viable typhoid bacilli in such preparations.

Using our spot method of titration the titre of this .prevaration is 3 x 409,
Plaques are fairly easily visible but small on surface bacterial lawns; they
may require a lens for satisfactory demonstration.

The spectrum of activity of this phage is very wide. It attacks every
Vi-phage type of the typhoid bacillus except Types 26 and k; the latter, of
course, is the strain from which the phage was isolated. It also lyses typhoid
C 901 and Salm. gallinarum, but I have not yet examined its action on other
Salmonellas.

IT should be interested to know what success you have with this phage as a

transducing agent.
Wit. Auced wpa (

(hewn,
Cross reactions of Salmonella paratyphi B typing phages

with heterologous Salmonellas.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Pool 2 Pool 2
Pool 1 Beccles, Tauntoy Pool 1 | Beccles, Taunton
Group | Salmonella |) 9) 3a, hewburn (now Group | @lmonella 5) 3a, | Newburn (now
Type 3al, 3b discarded ) Type 3al, 3b discarded)
Dundee, B.A.O.R. Dundee, B.A.O.R.
B  fbortus equif ++ ++ D sendai + + ++
stanley - + miami ++ ++
heidelberg - + onarimon - + +
istanleyville - + enteritidis ? + +
+ enteritidis
bredeny + + jena - +
cholerae
Cc suis - + + rostock - + +
1348
cholerae
suis - + =
kunzendorf + blegdam
5210
era - + + pensacola + -
daytona - + + clairbornei - =
para C .
( 9. stock - ++ italiana ~ =
cultures)
manhattan - zz javiana + + -
virginia - t E muenster - +
newport hitt - +
Puerto Rico ~ ++ ¢ Agons
fayta - + + G wichita - +
bovis
norbificans - + H heves - +
cornwallis ~ + I orientalis - +
+ + = semi-confluent to confluent lysis

+)

+)

diminishing degrees of reaction