OQ
INDIANA UNIVERSITY
BLOOMINGTON, INDIANA

DEPARTMENT OF BACTERIOLOGY

January 30, 1957

Prof. Joshua Lederberg

Department of Genetics

University of Wisconsin
Madison, Wisconsin

Dear Josh:

Apparently I have somehow missed requesting a reprint of your
paper in the Proc. Nat. Acad. Sci. 2, 57) (1956), May I have one
and, while you are at it, may I also havd reprints of such of your
bacterial genetics papers as are available? I have only the Heredity
2, 145 (1948) review and I am participating with Tracy in a bacterial
genetics seminar,

We have become somewhat bogged down in problems of protoplast
stability, etc. Our results became so irreproducible that we just
have had to back off and learn some more about the procedure and
general handling of the things. I can say pretty definitely that
it is obvious to us that our protoplasts are far from identical to
yours, We have tried by a considerable variety of methods to get
reversion with no success whatsoever so far. It is obvious that the
Versene causes irreversible changes alone in terms of viability of the
cells, We may switch to your method yet!

Talked to Norm Zinder a couple of weeks ago. I imagine you have
heard from him regularly, but at any rate he is rather off of
protoplasts at the moment. Sometimes I wish I was.

With best regards--

Yours. sincerely,

 

DF/er eee -