FEDERAL SECORITH) ACENCH/

PUBLIC HEALTH SERVICE
DEPARTMENT OF
IN REPLYING, ADDRESS THE HEALTH, EDUCATION, AND WELFARE

 

ior wunicable Disease Center
Enteric Bacteriology Laboratories

P.O. Box 185 April 16, 1953

Charblee, Georgia

Dr. Joshua Lederberg
Department of Genetics
University of Wisconsin
Madison 6, Wisconsin

Dear Dr. Lederberg:

Enclosed is the report on the cultures received from you
on March 13.

Some comments are needed. Note that Sw960 B, Swo88, and
SW891 B probably are induced phases of 1,2. Some reaction has
occurred with 1,20 serum but I am not sure that they are actually
1,10 phases. However, from their reactions I believe it is safe
to assume that they are changed 1,2 antigens. Unless you feel that
it is desirable we will not pursue their study further.

As you surmised, cultures 2821-52 and 1553-52 probably are
identical. One of these came from Commander L. A. Barnes at Bethesda.
The other was received from Kauffmann. The Barnes culture came from
Floyd in Cairo. Kauffmann's culture probably came from the same
source. Jt was said to have been isolated from fresh chicken OSES.
That is why Kauffmann thought it was S. gallinarum. I told him of
your results with these cultures but he has uot remarked upon them.

It does seem that you have transferred the suppressed a phase
of S- abortus equi to Kauffmann's culture 248. (SW666). This result
does not coincide with the speculation in my last letter that a phases
obtained from §. abortus equi might react as phase 2, just as T,2 phases
from S. paratyphi B var. java actoas:phaseri phases.

Your \SW977) does not agglutinate with any of our serums slthough
it is quite motile. I will prepare a serum for this phase although I

do not know exactly what we will do with it after it is prepared.
will send you some if you wish it. SW987 has caused some trouble. At
one time Miss McWhorter thought she had some agglutination with d sérum
but upon repeated passage in semisolid could not confirm it.) We must
study this culture further before making a final report.

 

   
 
   

Your SW98l1 is very interesting. JI was glad to know that Ba.
reacted as a phase 1 antigen. I think it is simply a changed b.
This culture was reversed in phase from Single colonies.

 
“Za
Dr. Joshua Lederberg April 16, 1953

I transferred a number of forms from S. abortus equi today. I
am in some doubt as to the identity of certain of the phases and must
look at them before I send them to you. I am sure that we will be able
to recover some a phases from the cultures. These may be useful in
studying the genetics of transduction.

The materials which you wanted will be sent as soon as we
can transfer the cultures and prepare the serums for shipment.

With kind regards, I am
For the Officer-in-Charge, Bacteriology Section

Sincerely yours,

PR. Ehasrm Bn

PRE :mg Philip R. Edwards, Ph. De
Pacteriologist-in-Charge
Enteric Bacteriology Unit
Bncle

 

 

 
P.S. From your last letter [ am not sure whether I can figure
out Sif 1031 and I do not have yours of 4/30 at hand. Please
return those pedigree sheets. JI have no copies.

P.S. 2 I have now looked at your letter of 4/30 and can decioher
Sw 1031.
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