YALE UNIVERSITY

OSBORN BOTANICAL LABORATORY
NEW HAVEN, CONNECTICUT

May 19, 1947.
Dear Dr. Mather--

The manuscript to which £ referred in my airmail letter is . |
enclosed. There is no pressing reason why it should be returned ee

unless that seems especially convemient.

 

Since that previous note, some data on a three-point test of os |
linearity have appeared, which, I think are in line with the
scheme you suggested-

The odharacters are: lLac- (lactose fermentation) yy! S|
resistance to phage T1) and ver/8 (resigtance}o phage T6.

In the cross : : aS

 

 

 

A 8&2 ¢ DLE a eof “ee
B-M- V¢ Lact ¥)" Tle X BtMt Ve Lact ¥,~ I-L- ee
Lng V The prototrophs (BAM+T+L+) fall into the following categories: . ;
iby 423 7 7 Z sorbate tl Fi 4 hea Anhly carl :
4 at 2 . Tia 2 4 alin puns ©
rosy - or rp 2343 lac -r+ = 41:20 Therefore Lao is
aaa . - s rer Tle ye ed to BeM es
a ae 7+Y By - 2 s 4}, 1 123 *D oe
ny Dees 7 - 3 s 8 5 ‘4 ¢ V-r:s = 46:15 Therefore V- is
0 6 May dS + 2 rep 5 2) ope linked to BoM
» O + 8s r l= & ; .
SG IG eg 8 dcyra— The data clearly show that Vg is linked’
8 + s S bt O97 3y to Lac (parentals?: new combinations
.\ ! are 54:7) These factors are therefore

| vag 443 | linearly linked. This is particularly.
Fry tft shown by the interaction with V, (e.g. 7 and 8)(or 1 and 3).
5 Y IS “+234

? © \24 The map therefore is: :
*BU

é

B, B-M Vg lac Vy, Td,

I realize that this is a rather small Sample, but it is already
extensive enough to be highly gratifying. em
Yo

 

=. “OShus Lederberg .