FEDERAL SECURITY AGENCY
PUBLIC HEALTH SERVICE
YOOSIOCROIK RE XBK

 

{IN REPLYING, ADDRESS THE
“UBLIC HEALTH SERVICE

REFER TO:

Tuberculosis Research Laboratory,
411 Yast 69th St., New York 21, N. Yo

March 10, 1952.

Dr. Joshua Lederberg,
Department of Genetics,

The University of Wisconsin,
College of Agriculture,
Madison 6, Wisconsin.

Dear Joshua:

Under separate cover we are sending you
cultures of our wild type W strain ,amd-one diauxotroph
and two triauxotrophs. The mutants have absolute
requirements except for the PAB requirement which is
slightly relative. The supplents we used in our tests
for their growth requirement were in p/m

L-tryptophan 20
L~histidine 40
Dl-methionine 20

L-proline 40
Di-leucine 4,0
PABA Ool

I'll be interested to hear how they behave in
your tests for recombination.

With best regards,

Sincerely yours,

Weems

Werner K. Maas

WKM/h1