july 11,1958

Professor J. Lederberg

Department of Genetics
University of Wisconsin
Madison 6, Wisconsin

Dear Professor Lederberg,

Thank you both for your very kind reply of may 5
and for the reprint from "Genetics" I received
yesterday.

I want to stress to you that the point on which
I insisted in in my expa@iment was that physiologi-
cal adaptation is impossible in resting clones.
My cellophane colonies were not moved across the
antibiotic -gradient plate until it was considered
that the colony has attained its full development,
this is to say that no further increase in its viable
count was liable to occur. WATANABE et al. /J.Bact.x
1957,74,770/ bave obtained essentially similar res-
ults using resting bacteria in buffered solutions.

Your paper on sib-selection is highly interest-
ing and I think it could be very useful for me in re=~ DH
combination expriments. c

I would be very grateful to you if you could send 4

me whatever reprints you may have available as recent iy
microgenetical litterature is rather scarce and "Ge-
netics" does not come at all. C

Yours truly,

Eugene Isaac Altescu

Laboratory Division

Dobrita Sanitarium
fg Jiu

(
Vie
MAD. ¥

Romania

EA/nor