OAK RIDGE NATIONAL LABORATORY

OPERATED BY

UNION CARBIDE NUCLEAR COMPANY
DIVISION OF UNION CARBIDE CORPORATION

3

POST OFFICE BOX Y
OAK RIDGE, TENNESSEE

February 19, 1960

via air mail

Dr. Joshua Lederberg aS |
Stanford University Medical School 3

Palo Alto, California a)
Fp tle

Dear Dr. Lederberg:

I am trying to track down non-lysogenic HFr and F- cultures for
some studies that we have initiated here at Oak Ridge. Dr. Luria
suggested that you might be able to help me out.

The project involves X-irradiation of one of the parents used for
a cross and an attempt at genetic analysis of the progeny. So far our
data suggest that X-irradiation of the F- increases the frequency of
recombinants obtained but this data is very much confused by the fact
that all of our cultures are lysogenic and are induced by the doses ;
employed. I feel that the system may be an interesting one to work 6
out, and I hope that you can provide me with some cultures and infor- i/
mation on them. I would prefer to use HFr's for which mapping is
pretty well worked out since one of our projects involves radiating
the HFr and scoring for cell viability and breakage of linkages between
genes as assayed by mating with unirradiated F-.

 

Thank you for your attention.
Sincerely yours,
Howard I. Adler
Biology Division

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