BROADWAY 2-1506

BURTON A. WAISBREN, M. D.

SUITE 33f BANKERS BUILDING
208 EAST WISCONSIN AVENUE
MILWAUKEE 2, WISCONSIN

*RACTICE LIMITED TO
-NTERNAL MEDICINE

March 29, 1952

JOshua Lederberg

Associate Professor of Genetics
University of Wisconsin

College of Agriculture

madison, Wisconsin

Dear Dr. Lederperg:

I want to thank you for your kindness on
warch L5th in showing me your equipment and exchang-
ing ideas.

I am enclosing tue sensitivity to 8 antibiotics
of the three strains you gave me. The fact that the
W1177 is streptomycin resistant is of real importance
ana iwight indicate a mode of action of streptomycin
in preventing the absorption of wnat ever amino acid
W1177 is deficient in. If you would be willing to
send we your wnole series of amino acid deficient
strains with the information as to what amino acids
they are unable to synthesize I feel it would be
worthwhile to ao the sensitivity tests on them.

We will keep you informed in regard to our
reconstitution experiments and hope in the future to
have the privilege of seeing you often. Thank Miss
Lively again for her patient help. You cooled me a
bit on my single cell enthusiasm but I still hope to
get the technique down well enough to start each
experiment with single cells.

Sincerely yours,

OUR wh rrr

burton A. Waisbren, M.D.

Baw: jem
Enclosure (1)
INFECTIOUS DISHASE CONTROL UNIT

MILWAUKEE COUNTY GENERAL HOSPITAL

 

 

 

NAME K eS HOSPITAL # __ WARD
The strain of isolated from the
(species) (source)
of the above patient on was inhibited by the concentra-
(date)

tions of antibiotics indicated below as determined by the tube dilution method.

—* T res rll Tr

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

38 of /
Inhib. |__-75 oy 5 Wa
cone.
AAg/ml. Oo :
3 CX |X x
6
12 O
25 if XK
50
100
“ 100 O
A ec | fa S P N Q 3B P
U HE |s T E E - A 0
R L | R R YN 0 1 ¢ L
E o | R E I M 9 I Y
0 R | A P C Y T M
M o 1M T I C R Y
Y M | Y 0 I I A x
C y jo mM | oo YN 6 I
I clr Y I I V
N zg iw c N N -
T I B
I N
N
I
Remarks: