UNITED STATES DEPARTMENT OF AGRICULTURE

AGRICULTURAL RESEARCH ADMINISTRATION

BUREAU OF AGRICULTURAL AND INDUSTRIAL CHEMISTRY
PEORIA 5, ILLINOIS

February 5, 1948

Dr. Joshua Lederberg
Department of Genetics

The University of Wisconsin
Madison 6, Wisconsin

Dear Joshua:

I eam glad to be of some assistance to you in the interesting work
you describe in your letter of January 27. Under separate cover
I am sending you samples of calcium lactobionate, calcium malto-
bionate, calcivm 2-keto-d-gluconate and calcium-d-xylonate. If
the amounts seem rather smell, it is because analytically pure
samples are rather hard to come by with this type of compound
although we can easily prepare the crude material (ca 90 percent
purity) in kilogram lets.

The analytical data is as follows:

Calcium lactobionate 2512-29-A (3.0 grams)

 

This sample has 8.68 percent moisture. On the dried semple the
values were:

c oa
Calc. 38.19 5.€1 5.31
Found 38.0 5.95 5.22

Hydrolysis data on the dried sample showed 96 percent purity.
The lactose on the undried sample was 0.25 percent.

Calcium lactobionate 2378-7-A (200 mg.)

This sample has 5.58 percent moisture. On the dried sample the
values were:

& # ce
Calc. » 38.19 5.61 5.31
Found 38.0 5.58 5.38

The rotation on the dried semple was +25.1°. The compound has no
detectable lactose.
2-Dr. Joshua Lederberg-2-5-48

Calcium maltobionate 2378-8-A (1.00 g.)

 

This sample has 5.22 percent moisture. The values on the dried
sample were:

¢ ae
Calc. 38.19 5.61 5.31
Found 38.1 §.61 5.58

The rotation on the dried sample was +103°.

Calciun 2-keto-d-gluconate trihydrate 2101-1-4 (4.0 g.)

 

c # Co Hg0

Calc. for
trihydrate 30.0 5.04 8.34 11.2
found 30.1 5.08 8.31 11.5

Calcium d=xylonate 2292-19-A (400 mg.)

 

This sample has 6.70 percent moisture. The values on the dried
sample were:

c Ho Ga
Calc. 32.43 5225 10.82
Found 3207 5.25 11.2

Calcium d-xylonate 2292-19-B (2.0 g.)

 

This is the second crop from the filtrate from 2292~19-A. We have

no analyticel data on this sample, but we have reason to believe it
would be good enough for preliminary experiments. We would appreciate
it if you would send it over to your Chemistry Department for carben,
hydrogen, and calcium analyses and then forward the results to us so
that when we get future requests we can indicate the quality of the
product. ,

I hope these samples will be of assistance to you, and I wuld
appreciate it if you would keep me informed of the progress of
your work. It interests me very much.

Best regards.
Sincerely yours,

red te Sted,

Frank H. Stodola, In Charge
Chemistry Section
Fermentation Division

Enclosure Northern Regional Research Laboratory