UNIVERSITY OF CALIFORNIA

LOS ANGELES 24, CALIFORNIA November 28, 1951.

Dr. Joshua Lederberg,
Department of Genetics,
College of Agriculture,
University of Wisconsin,
Madison 6, Wisconsin,

Dear Dr. Lederberg:

Thank you kindly for your very
interesting letter of November lst which I failed
to acknowledge promptly.

Under separate cover I have sent you a trans-~
plant of the Smith strain of Staphylococcus aureus
which was used in our transformation experiments.

The medium we used was not by Chapman and
Stone but by Chapman and Stiles. It is a phenol
@ red agar base which was sterilized in an autoclave.
Then concentrated solutions of the various carbo-
hydrates were filtered through Seitz filters and
added aseptically to the melted agar base. The
reference is:

Chapman, G.H., and M. Stiles: Carbohydrate
fermentation reactions of staphylococci,
Am. J. Clin, Path., 10:380, 1940,

I hope you will be able to check our results.
I plan to continue the problem when I can interest
a suitable graduate student,

With kindest personal regards,

Very sincerely yours,

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@ Co AL OL*BaTL Chairman
ajs.s Department of Bacteriology