September 26, 1953 ( Dr. J. L. Howatt 1 | Drawer 940 i Fredericton, N.B. S| Om Dear Dr. Howatt: 1343 9 (thr netics,32:514, table 5]) sbated |to require eonine + methionine, received from B.D. Davis. /) — “a We1361 "xX prolingless . m§.4n 58-161 [Proc. Nat. Acad. Sci.US 31:217)) proling + methionine. also from Davis. 34s and vi 61 are both ultimately derived from E, ecli strain K-12, a - lineless from FE. coli strain B] w-1669—~"leucineless " " n " nj from A. Nowdek These strains hhve not recently been checked for purity and authenti-~ city. Please call ay attention to any anomalous behavior. I am also sending some reprints covering techniques for the isolation of auxotrophic mitants. Yours sincerely, Joshua Lederberg