DIVISION OF SCIENCE SERVICE BOTANY AND PLANT PATHOLOGY DEPARTMENT OF AGRICULTURE Drawer 940, Fredericton,N.B., August 6,1953. Dr. J. Ledeberg, Department of Genetics, University of Wisconsin, Madison, Wisconsin. Dear Dr. Ledeberg: I have been searching over the Continent for a culture of E. coli, which has a requirement for proline. Have you and could you favour us with a sub culture of the organism,or could you direct us to some source having this material? Yours very truly, 1% , @ Poy IR J. L. Howatt, Plant Pathologist. J LH/VL