I2

YALE UNIVERSITY
OSBORN BOTANICAL LABORATORY
NEW HAVEN, CONNECTICUT
:
- — i
Setober le, 1946.
Ure
Leot
Univ
Toma:
Lear

has been kind enough to aliow me to see a Cony

 

of your manuscript: "fhe Statistical Theory of Sacterial sutations.t

This peper wes especially interesting because Dr. 7,J.Ryan {oz

Colushia University) ani I have been stud; ying the reverse-mutations

of biocneralesl mutants < of bacteria, «nd have ha

to estimate rates of mutation from the frequency of mutants oy the

cusio nm to attenpt

f
QO
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general procedures described by Delprick.
in the course of this work a satnegathcal protlem nas arisen

concerniny ~nieh we should lixe to elicit your opinions or sussestions

 

on ee aay we + wat o . x t a has-e - ag
The experimants of Lurie and Celbritex revecle ad large difverances in

Oo

™ culture to culture, the lerge value of
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r
te variance being ascriced entirely te the a-noraal distridution

awe ft oe 7 nee camry ot a wom ve de tee ps ~ tys ayy L

conditioas. It was moped to vest this nypothesis oy using double autan:
: Ne ey Foes An a 3 3 2 a " e east 7 4

of becteria, und ceternining, in different sSenples oO: a siven culture

tig tre uency of reversions at eech locus. initicily, aAcstances or co-

<q .. os
reversion rates of cae 107°.) Then data were accuaulated froa whien we
noped to be able to draw inferences concerning tue cerrelition of muta-

tions et each Iscus to the otner. If there were uncontrolled difrerence
 

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Hespectfully yours

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