March 20, 1950

ee

Miss Phyllis Fried
96 Avenue C
New York 9, New York

Dear Miss Fried:

I have your letter of the 16th and am glad to welcome
you into cur laboratory group. — .

Your stipend, which should be $950. for the academic
year will be paid in ten equal mnthly instalments beginning
early in October. Students here usually find it advisable
to have between two and three hundred dollars on hand in
order to meet tuition and living expenses before the first
payment. In cases of need however, it is usually possible
to arrange short loan loans on short notice. An

It will be by no means necessary for you to antieipate
your graduate work at this time and I would recommend rather
that you concentrate on the advanced course work in bactsrial
physiology, that I believe you mentioned earlier. However,
4f you find yourself with an unusual amount of free time available
you might enjoy conducting some crossing experiments with
bacteria. I am sending you, under separate cover, two strains
which will be suitable for this purpose. 58-161 is a mutant
of E.°Coli which requires’ the growth factors blotin and acthionine.
W-1177 4s another miit8H$, requiring threonine, leucine, and
thiamin. It also carries a number of other useful genetic markers
including: inability to ferment lactose, maltose, galactose,
xylose, mannitol; resistance to streptomycin and to the baoterto-
phase T=}: I am also sending you a rather complete set of
publications dealing with the background of our current work
in bacterlal genetics. I recommend that you undertake to begin
to read the literature in this field as soon as possible and
for this purpose the review articles may be helpful. The other
papers contain, I believe, an adequate description of the
techniques that you will be likely to use in your crossing ex-
poriments as well as in your later work here.
The cultures that you will receive can be mde freely
available to anyone that can make intelligent use of then,
but I would appreciate your mentioning any further distributéon
of then.

Please do not hesitate to continue to correspond with me
on any matter on which I can be of any assistance.

Very sincerely,

* few.

JoshuhaLederberg
Assistant Prof. of Genetics

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