Sept, 7. 1955 Dr. Joshua Lederberg. Dept. of Genetics, Univ. of Wisconsin. Madison Wisconsin. Dear Dr. Lederberg: I would greatly appreciate it, if you could send cultures of E. coli K-12 and a "lactose-negative" mutant of K12. We are hoping to use these organisms to study the relationship of DNA to enzyme formation in cell free systems. Thanking you for your kindness. Very sincerely yours, Kaney Ebisuzaki.