Novamber 5, 1951

Dr. M. Doudoroff

Dept. Bactericlogy
University of California
Berkeley 4, California

Dear Mike:

Not long after ay last letter was poated, we received « note from
Ruger who had spcken to yuu over the phone. Along with Roger, Esther
and I were very much heartened at your response to your loss. We don't
have tc tell you that we've behind you.

I bhope the enelesed elreular is what you're looking for, Your strains
shoglda be ok; Kees shculd have them also.

For class purposes, the experiment could possibly te simplified further,

if each student io tc handle it himself. It is not essential to use

@ washed suspensions, although you got o somewhat hoovler background. The
parent cultures shovld oe grown separately; overnight in Pennassay broth.
The merely slate ebcut .l gh of onch parent, together, or separately for
controls, on minimal agar. Or, growth sersped from a streak of growth
on nutrient cgar wlll algo providcs = ressonably gocd inoculun. There is
nothing fastidicue about the conditions for recombinatdion,2t all, so,long
as theytultures haven't been sitting sround too Jong.

The Glu- certainly appears to be perplexing. I hore that there is a
detailed discussion of your experiments in the Penetabolisn paper; if not
I shall expect to get the fullest details from you when you visit, which
I am looking forward to. This 4s one case, anyhowy where you can't stand
on permeability, unless you want to extend that concept to dried celle! sHave
you explicitly tested the utilization of phosphcrylated glucose intermediates,
not step by step, but for overall glycolysis?

We do have W-108, W-327 etc., or thelr equivalents, and will be glad to
send anything out to you again that you might have any use for.

The lactose+ hexose- has pretty well flopped, Contrary to reactions on
EMB agar, the lactose-grown cells (in a crude experiment) fermented glucose,
lactose, galactose in that order. Probably it will boil down to (mal) adaptive
ppecificities, along the mix order of P. putrefedhans and maltose.

There's lots more to talk about, but will keep until we see you.
Sincerely,
© . girl, Emily Woodworth Bailgy, ever show abpuas a
“er student (undergrad) from UW, or to look for a
~ used to assist in my research, and was a Joshua Lederberg

JL
ee