Massachusell, General Hespdlal

GENERAL HOSPITAL ZB. / MCLEAN HOSPITAL
BAKER MEMORIAL TA

W. FRANK
PHILLIPS HOUSE ancoe M.D.

IN BOSTON

 

 

BURNHAM MEMORIAL FOR CHILDREN
HALL- MERCER HOSPITAL
HUNTINGTON MEMORIAL HOSPITAL (convacescentas
VINCENT MEMORIAL HOSPITAL

IN LINCOLN

 

Dean A. CLaRK. M.D.
GENERAL DIRECTOR

January 6, 1953

Dr, Joshua Lederberg
University of Wisconsin
Madison, Wisconsin

Dear Dr, Lederberg:

The Sglmonella strain 'Skinne# which I am sending under
separate cover was isolated recently from an epidemic of food
poisoning. It was typed in the Salmonella center in New York
as 'Montevideo'., It produces L forms abundantly on soft
serum agar containing penicillin, but the L forgs grow quite
slowly. The two pieces of agar in the small tubes should be
taken apart and placed with the inner side dow on the mediun,
With the low power of the microscope the L colonies are visible
in some of the agar pieces. You should try the usual nutrient
agar to which should be added 20% broth, 15% inactivated horse
serum, 1% gelatine and a few hundre@é wits of penicillin per ml.
If the medium is too soft try preparing it with a little less
broth. Make the plat robic with B. prodigiosus. Growth
will probably devel opt eatee the agar blocks in 2-3 days. More
material can be obtained by smearing the blocks at this time
over the surface of the gm medium. If you should have difficulty
with the cultures, I will send another sample.

Thank you for the reprints. I know the complexity of
biological phenomena, It is overwhelming in the field whic}
you reviewed,

With kind regards,

Sincerely yours,

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