GENERAL HOSPITAL ZB \ Dh MCLEAN HOSPITAL
BAKER MEMORIAL y W. FRANKLIN Woop, M.D,

PHILLIPS HOUSE DIRECTOR

 

 

BURNHAM MEMORIAL FOR CHILDREN
HALL-MERCER HOSPITAL
HUNTINGTON MEMORIAL HOSPITAL
VINCENT MEMORIAL HOSPITAL

IN LINCOLN

STORROW HOUSE
(CONVALESCENTS)

 

DEAN A. CLARK. M.D.
GENERAL DIRECTOR

December 11, 1952

Dr, Joshua Lederberg
University of Wisconsin
Madison, Wisconsin

Dear Dr. Lederberg:

I am forwarding the Salmonella L strain which is now growing
fairly well on 10% horse serum agar. We are adding 1% gelatin to
the media but I do not know whether it really helps. The cultures
are on one side of the agar squares and you should streak them on
fresh plates and leave the squares on the plates, Growth often starts
only under the agar squares. This is a 3A culture which does not revert
to the bacillary form, You can get 3B L forms without difficulty
from the Salmonella if you inoculate nutrient agar,containing penicillin,
heavily and make pour plates. These cultures can be -propogated only in
pour plates.

The culture which you obtained from strain 52 is also of the 3B
type. If you transfer it on nutrient agar with horse serum and
penicillin you can get the 3A,

I will make some experiments with your W-1637 when it arrives.
It is probably in the Christmans mail,

I am very much interested to know whether you have any success
with. the Salmonella L. I hope you will keep me informed.

With kind regards,

w/ Sincerely yours,

ogres iets