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THE UNIVERSITY OF TORY L

 

 

DEPARTMENT OF BIOLOGY WR 2 7985 }
FACULTY OF SCIENCE &, 5
Hongo, Tokyo 113, Japan Fi a
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Laboratory of Genetics April 3, 1985 / deficient mutants etc., we may examine their reactions to acridines
Professor J. Lederberg and correspond them to the mutant sites.
Director
The Rockefeller University Another approach to corelate the difference of phase 1 and
1230 York Avenue * sae
New. York, N.Y. 10021 phase 2 with the reaction to acridines may be to look for the difference
U. S. A. of physico-chemical characters between two phases. By now, physico-

Dear Josh : chemical studies of Salmonella flagellins have been confined mostiy

on 1.2 or enx, and the data on the phase 1 counterpart is scanty for
Sorry the delay of my correspondence to your reference on phase 1 extracting the difference between phase 1 and phase 2.
vs. phase 2 reactions to acridines. As I told you when you visited us,
I have been abroad to attend the 13th Aharon Katzir-Katchalsky Conference Sincerely yours
held in Israel and to visit several institutions in Europe and returned
home last week. It was my first visit of Israel, and not only enjoyed ro>

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in fewd —_— ee

Tetsuo Iino

the conference but also greatly impressed on the activity of the country.

As far as I know, no relevant informations to explain the difference

of reactions to acridines between phase 1 and phase 2 have been reported

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since Aleck and your report in J. Bacteriol. (1955). ( f L

As you may know, McDonough (1965) reported amino acid composition
of a number of falgellins of different antigenicity. But as he discussed, - - . ty depute ?
‘the phase 2 flagellins (1.2 and enx) did not differ systematically 19) a howe artlons fo SEAT UNC
from the various phase 1 flegellin'. In order to consider the difference {
in the reaction to acridines, informations on the amino acid composition -
or preferably amino acid sequence of the specific region of flagellin
polypeptide might be significant rather than the over all amino acid
composition. However, such trial has been retarded because of the
insolubilization of the peptide digests of falgellin. Now we are in the
situation to overcome the problem by sequence analysis of the cloned DNA

of the flagellin genes. The base sequence analysis of Salmonella
flagellins so far carried out is limited to 1.2 by Simon's group and

 

partially i by our group. If you are interested in the line of studies

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I will be glad to discuss the possibility of collaboration. As we have © be Ae be
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various flagellin mutants, such as the small molecular mutants, excretion