Department of Micrabiolagy Medal Center
Morcartowr, West Virginta
26306

January 28, 1985

West Virginia
University
Dr. Josh Lederberg

1230 York Avenue
New York, NY 10021-6399

Dear Dr. Lederberg,

Thank you for your note and your interest in our article on E. coli
hemolysins.

I must apologize for not being aware of your observations on the
hemolytic effect of E. coli lysates and the work by Schiff and Bornstein
on typhoid cultures.

Lysis by osmotic shock or sonication does release an intracellular
form of hemolysin and a form bound to cell membranes (B-eor cell bound
hemolysin). These hemolysins appear to be precursors of the » or extra-
cellular hemolysin. We have a monoclonal antibody to the hemolysin
which could identify the mechanism of lysis.

Respectfully,

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Irvin 5. Snyder, Ph.p,
Professor & Chairman

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