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UNIVERSITY RESEARCH COMMITTEE

REQUEST FOR RESEARCH SUPPORT FOR THE YEAR 1958-59 MUST BE IN THE

GRADUATE SCHOCL OFFICE NOT LATER THAN JANUARY 17, 1958

(Please submit forms in duplicate)

 

 

Name LEDERBERG ° Date
Last First Middle Initial
f Genetics
Department Madson?

 

Title of project _ G@netics off Bacteria

1. Freliminary evidence that the flagellar antigenic phase of
Results to date: geimonella can be altered by exposure of the cells to acriflavine,
As there is other evidence that the 'nhase' depends on the functional state of a
chromosomal factor, this is a rather exciting lead for developmental genetics,

2. No success to date in attempts to transfer renetic markers
to protoplast recipients by DNA; these experiments continuing. A mumber of wall-
defective mutants have been isolated. So far, these are all auxotrophic for fiaminoninelic
seid; others have not yet been characterized,

43, Preliminary evidence that crossing-over is reciprocal in
clones from single zygotes in E, coli. In contrast to other markers, the F agent
(fhhich determines sexue]l compatibility) 1s distributed to the entire exconjurant
cione in F x F" crosses, suggested that it is an extranuclear particle,

4, A variety of Hfr stocks have been isolated, and characterized
as having different patterns of genetic exchange.

5. In certain complex situations, serreget_ion of prophare in

Souplin with linked Gal markers has been found.
Procedure - indicate the essential working plans:

We propose tc continue along the same lines as used for the current program, (1) requires
proof thet the alteration is an induced change of phase, rather than selective survival
of cells already in one phase, (2) may need a new aprroach, rossibly the use of cells
damaged, but still viable, after ultra-sonication (for the occurrence of which Dr. Rotman
had some evidence). We will also try preparations made from transducing thage, as well

as from intact donor bacteria, New mutant blocks will also be sourht, particularly for
auxotrophs responding to nucleotide-erino acid cenjugates (in cooperation with Strominrer
at Washington University). (3) we intend to apply the interrupted mating techniaue
developed by Jacob (Paris) for the analysis of these Hfr'a, and to attempt to reconcile
certain differences in detailed interpretation, But as already stated, no radical
devartures are contemplated in advance.

The work is all intra~mral laboratory work, the essential facilities for which are

at hand, We are sovew-at hampered, at present, in chemical operations; for the coming
year the work will continue to he carried out in the Genetics Bublding, vith cooneration
where indicated, with other faculty,

Budget - (OVER)
Budget -

Staff | era ineunbente in the laberretory

Research Assistants (A $1920; Acad Yr $1600)

Ann ©, Cook |
Aler Dichter |
Yutinord "irote *

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Other Serriying Pabp. 1988

** crebebly L, Wnierie Janee ('.5,, Bactorfolory, ‘elbourne Univ, }
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*List names only if it is fairly definite the person will be
available; otherwis@ open positions should be indicated,
Hourly Help
g 1 oC
Supplies end Equipment
= *20
& -

TOTAL REQUEST