Maroh 13, 1953

Or. D, 8. Bruner

Dept. Vaterinary Sclonce
Cornell Univeraity
Ithaca, ".Y¥.

Dear Dr. Broner:

I wight mention firet that I recently spent a couple of weeks
at CShanblee with Dr. Edwarde, where T had a chance tc bolster my
very woak background dn Salmonella serology, A macher of probleme
have come im cut of that werk, and ainee, for which Shil suggested
you tight have some meteriaele thet would complement hades own. In ad-
dition, I have tried to summarize a few other items which may possibly
cons te your attention, and for whieh I would be most grateful if you
were able te crovids ther.

1. Se naret4. Savavds! sinele etock oulture is mouitable. Tt
was thought that it might be a good idea to try to sei up « monophasic
la,,1-~ by transduction. Qo you havo any others (up to half a dozen or
a 4 zen eoull rrofftahly be tested).

2, fe abortus-ecul. The stoat, #26, is giving some interesting
resuite I would like to try to generalize with two or three others.

3, --81,2 chasea in grow B for De Tt have alroady Ky. stock FS?
and twe or three rere recent from 244i (ineluding #191: *thefll"), whiek
era cll hebeving quite differently, and ecerfugingiv. Have you seen any
otnore?

4, say stable nom-metiie variants (excent, of courses vyllomargaliinaru).
We are working ur methede for the transduction of moti i:y, so as to unmaaic
the flagellar antigens, and eran already do thie retuer generally in BD
groups. For exampla, an outbreak of en untypeabie grow D in oalwee and pige
in Guatemala, in 1951, proved to be all S. dublin. J weuid like very much
to be able to extend thin type ef etudy, and would be equsily interested in
wiscollanecus eaetraine, and in any collection from s eingle o:threak. We hope
eventually to be able to handle the other @ groune, es wel! as Band D, so
thet any material at all would be welcome in ao far aa it dose not duplicate
what Phil has already placed at my dilapogal.

5. I have been reading your reaent notes on phase changes with creat
4nterapt, May I gount on a periodic exchange of relevant papers?

I fear thie letter ie all ask, and no offer. If? I can reciprocate in any

way, Please let me ‘nov, Youre sincerely.
J 3

Joshua Lederberg
Assootate Professor of Genetics