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Nuclear Staining Technic

Moetericls:

Buffer KHoPO, «¥/15, 01078 gm./100 ml. use 4 ml. in about 200 1.
NeaoHPoh 4/15, 0.947 pm./100 ml. use 6rl. distilled water

Abovon mounting meteriel: Glyco Products Co., a weter-soluble resin.

Setursted aquecus mercuric chloride (sublimete): use 2 rerte in 1 rert abselute
ethyl alechol.

Lml. conc. Sl plus 9 ml. dietilled water makes ancrox. N/l.
Method:

l. “ix bacteria (in honging drop or on sector of egsr) in 2% osmium tetrnoxide
for 21/2 to 3 minutes. De not dry cell’ until after fixation.

2. read on coverslips or meke imrression smear. Ory 10 seconds.

3. Rebinew uses atiitionsal fivetion fer 1 1/2 mim. in Hgllo , followed by
washing in severel changes cf 95° cleohol and water.

A. Tleee slide in cold “ANN for 69 aceonda.
5. Yot Wl (about 60°0.) for 10 mimtes. g2cret

6 old Hl for about 20 seronde.

7. “Vash several times in buffer, ri 7. sdiog2 + 2 dager Ete fyy or
8. Stein for 20 minutes in emer, Uloted 1-190 or 1220 wit! buffer. Pappa -

(Ordinary commercial Giemsa usanlly remiires stronger seln. )
9. ash briefly in buffer.

10. “fount in water or Aboron (diluted 2? rarts in 1] rart water).
 

 
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