Yukio Bito
Dept. of Animal Microbiology,
University of Osaka Prefecture,
Sakai, Osaka, Japan.
1973. Jan. 8.
Dr. Joshua Lederberg
Genetics Dept., Stanford Univ.
Sch. of Med. Sch.,
Stanford, Calif, 93405.
Dear Dr. Lederberg.,
Recently, the study on the relationship of the chemical structure of bacterial lipopolysaccharide to its biological functions has been initiated in our laboratory using a mild strain of E. Coli. However, the mutants lacking various constituents in the LPS structure are necessary for the fully successful investigation.
Would you send me galactose negative mutants of E. coli, and mutants defective in heptose in addition to hexose, if available?
Thank you in advance,
Yours sincerely,
Yukio Bito
[written in Lederberg's handwriting, W2375 gal4 lp plus
W2374 gal2-lp plus
W3298 (from Jacob)number P699 gal-Sr galb
W3925 gal- (t) ust Hfr
W2236 gal3-lac-lp25
W2358 lambda5 gal1minus]