PROPOSAL FORA

GENETICS RESEARCH CENTER

Submitted to the

National Institute of

General Medical Sciences

June 1, 1973

School of Medicine
Stanford University
Form Approved

 

SECTION | Budget Bureau No. 68-RO2¢69
DEPARTMENT OF LEAVE BLANK
HEALTH, EDUCATION, AND WELFARE TYPE NUMBER

PUBLIC HEALTH SERVICE

GRANT APPLICATION

 

  
  
      

REVIEW GROUP FORMEALY

 

 

COUNCIL (Month, Year) DATE RECEIVED

 

 

TO BE COMPLETED BY PRINCIPAL INVESTIGATOR (items 1 through 7 and 154)

1. TITLE OF PROPOSAL (Do not exceed 53 typewriter speces}
Genetics Research Center

 

2, PRINCIPAL INVESTIGATOR
2A, NAME (Last, First, ‘ritial)

Lederberg, Joshua

 

\F ROM

3. DATES OF ENTIRE PROPOSED PROJECT PERIOD (This application)
THROUGH

1/1/74 12/31/78

 

28. TITLE OF POSITION

Professor and Chairman
FCO MAILING ADDRESS (Street, City, State, Zip Code)

Department of Genetics
Stanford University School of Medicine
Stanford, California 94305

     

   

321-1200 Ext. 5801

3G. DEPARTMENT, SERVICE, LABORATORY OR EQUIVALENT
{See instructions)

Depts. of Genetics and Pediatrics
TH. MAJOR SUBDIVISION (See Instructions]

N/A

 

 

4. TOTAL DIRECT COSTS RE-
QUESTED FOR PERIOD IN
ITEM 3

$2,659,305

5. DIRECT COSTS REQUESTED
FOR FIRST 12-MONTH PERIOD

$537,800

6. 'T See Instructions)

Department of Genetics and
Department of Pediatrics

Stanford University School of Medicine
Stanford, California 94304

 

9- Research Involving Human Subjects (See Znstructons)

A.C) NO 8.7) YES Approved: _
C. Gd YES — Pending Review -29=73 Date

TO BE COMPLETED BY RESPONSIBLE ADMINISTRATIVE AUTHORIT

4. Inventions (Aenewa! Applicants Only - See Instructors)

A.CINO B.[_] YES — Not previously reported
C. (CIVES — Previously reported
Y {items 8 through 13 and 158)

 

a APPLICANT ORGANIZATION(S! (See instructions)

Stanford University

Stanford, California 94305
IRS No. 94-1156365
Congressional District No. 17

16, NAME, TITLE, AND TELEPHONE NUMBER OF OFFICIALS) |
SIGNING FOR APPLICANT ORGANIZATIONIS)

Kathleen C. Butler
Sponsored Projects Officer

41. TYPE O

 

 

F ORGANIZATION (Check applicable itern)

COFepeRAL C)stTate [LOCAL (3) OTHER (Specify!
Private, non-profir

NAME, TITLE, AODFESS, ANO TELEPHONE NUMEZA OF

OFFICIAL IN BUSINESS OFFICE WHO SHOULD ALSO BE
NOTIFIED IF AN AWARD IS MADE

K.D. Creighton
Deputy Vice President for Business & Finance
Stanford University
Stanford, California 94305
Telephone Number (415) 321-2300 _X2251

A222 /2=—
i A AL COMPONENT TO RECEIVE CREDIT
FOR INSTITUTIONAL GRANT PURPOSES (See instructions)

O01 School of Medicine

 

 

c/o Sponsored Projects Office

14, ENTITY NUNBER (Formerly PHS Account Number)

458210

 

Tetephone Number {s)__ (415) 321-2300__Ext. 2883

 

1& CERTIFICATION ANO ACCEPTANCE. We, the undersigned, certify
knowledgs and accept, a3 to any grant ewerded, the obligation
averd.

that the stetements herein are true and compiete to the best of our

to comply with Public Health Service terms and conditions in effect et the time of the

 

SIGNATURES A. SIGNATURE OF PERSON NAMED IN ITEM 2A
(Signetures required on oof ty Nn eTinee wh eg oy
origina! copy only. Te. SIGNATU

Use ink, “Per” signatures
not ecceptzbie)

 

'S} OF PERSON(S.
iden 7

 

ap ITEM 10
t -

(See also page i)

DATE

MAY 39 1973
GATE

5/30/73

 

 

 

WIN 398 (FORMERLY PHS 398
SECTION 3

DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE LEAVE BLANK
PUBLIC HEALTH SERVICE PROJECT NUMBER

RESEARCH OBJECTIVES

NAME ANO ADDRESS OF APPLICANT ORGANIZATION
Stanford University, Stanford, California 94305

 

 

 

 

NAME, SOCIAL SECURITY NUMBER, OFFICIAL TITLE, AND DEPARTMENT OF ALL PROFESSIONAL PERSONNEL ENGAGED ON
PROJECT, BEGINNING WITH PRINCIPAL INVESTIGATOR
Joshua Lederberg, Professor and Chairman, Department of Genetics
Howard Cann, Associate Professor of Pediatrics, Dept. of Pediatrics
Norman Kretchmer, Professor of Pediatrics, Department of Pediatri
Leonard Herzenberg, Professor of Genetics, Department of Genetics
Luca Cavalli-Sforza, Professor of Genetics, Department of Genetics

Luigi Luzzatti, Professor of {atrics and Community and Preventive Medicine,
Department of Pediatrics

Clifford R. Barnett, Associate Professor of Pediatrics, Professor of Anthropology,
Department of Pediatrics (CONTINUED BELOW)

TITLE OF PROJECT

 
 
 
 
  

 

GENETICS RESEARCH CENTER
USE THIS SPACE TO ABSTRACT YOUR PROPOSED RESEARCH, OUTLINE OBJECTIVES AND METHODS. UNOERSCORE THE KEY WORDS
(NOT TO EXCEED 10) IN YOUR ABSTRACT. .

A comprehensive program of basic and clinical research, uniting the efforts of
the Department of Genetics attd Peadjatrics, will apply advances in analytical
instrumentation to problems of genetic polymorphism and disease in man.

 

Program areas include:

Genetic errors of metabolism identified by computer-managed gas chromatography
and mass spectrometry, applied to urine, blood and amniotic fluid;

Detection of fetal cells in maternal circulation;

Expression of genetic markers in fetal cells, linkage studies; antenatal diagnosis;
Polymorphisms involving specific binding to plasma proteins;

Psychological impact of genetic disease and counseling practices.

 

 
 
 
 

* Kenneth Tsuboi, Sr. Scientist, Department of Pediatrics
Alan Duffield, Research Associate, Department of Genetics
Wilfried Pereira, Jr., Research Associate, Department of Genetics

 

LEAVE BLANK

 

PHS-398 PAGE 2
Rev. 3-70
The anijersigned agrees to accept responsibility for the
scientific aud technical conduct of this project and for
provision of required progress reports if a grant is awarded as
the result of this application.

 

Le
MAY 30 973 Need ave To el
fc \
Date Y Joshua Lederberg, ~~

Principal Investigator
TABLE OF CONTENTS

I. Introduction and Program Summary ....cceeecee

eee es ene

Comments on Organizatlon .iccccccvecvvecesssvsvce

The Stanford Environment ...ccsoes

Clinical Factilittes ceocccc ccc ccc cnc ccscccccccves

Research Program Outline wccvnccccncvsscescccsces

t1. Screening and Charactertzation of Inborn Errors of
Metabolism by Gas Chromatography/Mass Spectrometry

Analysts of Body Fluids

(Drs. Lederberg, Kretchmer, Cann, and Nuffield) .....

276.2 Budget and Explanation e@eeovovveeveseeeeaeeeeneseeeseeee ee eee

tlt. Maternal Blood Stream - Arother Source of Fetal
Tissue for Pre-Natal Diagnosis of Genetic
498 Disorders (Drs. Herzenberg and Cann) ouosesevreveceesere

Budget and Explanation eoeveveoeveveevnenee oes eeseew eevee eoeeae ee @

{1V. Polymorphic Genetic Markers in Amniotic Fluid
(Ors. Cann and Tsubol ) eseeoveveeevpeeevne eevee scoeoevneevnevnane eee ees

Budget and Explanation eeseeeoeneeeeneeneneaeeaewaoaeaeee ee ee

Vv. A Search for Genetic Polymorphtsms and Variances

Of Specific Binding Proteins

in Blood

(Dr. Cavalli-Sforza) eeseveaevee even eevese ene seen oeveeee ene ee eee

Budget and Explanation eeoeseveeoeveeoeoaneneveneanenesnaeansvseos eee ee

Vi. The Impact of Genetic Counseling Practices on

Family Decisions and Behavior
(Ors. Barnett, Cann, and Luzzatti)

eoeoeaeaevoee eee eevne ee ee

Budget and Explanation oeeoeaesveosvseoeoseooe eevee e eee ane ese eee

Vil. Overall Budgets esenvnaenvnesneveveeer eevee eeeevreeeaeevneweevee eve

Program Director's Office Budget ...ccccececcsecs

Aggregate Genetics Research Center Budget .......

VIET. Concluding Remarks (Dr.

Lederberg)

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P-17
P-78

P-85

p-97

P-190

P-119

P-122
P-139

P-133
P-145

| P-lh8

p-149
P-152

P-157
-3-

TABLE OF CONTENTS (cont.)

X. Professional Personne] eeeseee#ez#see#eteee»eeeeseeeveenreeeeeeeevs#ete¢e P-15690
Key Personnel and Backgrounds ...cccscccccccecseses Pr1El

Relevant Research Support Summary for
Departments of Genetics and Pediatrics .....cseeee. P-165

Individual Biographies eoeoeoeereovnesesneeereeeeeeseererve P-169