ARE Ho AND PHAS:; CONTROLLER SEPARABLE BY RECOMBINATION ?

Aimonophasic culture of (i):e.n.x type obtained from 'Sal. abortus-equi (a):
€,9,x —-x Sal. typhimurium i:1,2' was transferred to anti-e,n,x serum NGA deep
tube. i:(e,n,x) type of monopliasic culture was selected from the deep tube.

In this monophasic type, Hp is expected to be stabilized in inactive state.
Consequently, the following transduction experiment will produce swarm only

when recombination occurred between Ho and phase controller.

 

Sal. typhimurium i:1,2 -—-x the i: (enx) type ---~3—-—~—sp (i):e.n,x or none ?
anti-i, &-1,2
NGA.

Experiment.

A lysate of Sal. typhimurium TM2 (titer 4.5 x 10?/m1) was mixed with equal
volume of penassay broth culture of the i: (enx) type and the mixture was droped
on anti-i,l,2 NGA deep tubes, 0.1 ml in each. So far, twenty five deep tube
cultures have been tested. None of them produced swarm. That is, no evidence

of recombination between Hp and phase controller has been obtained.