928

19, Loss

r ad al
Peapics tat
Bune tes L355

v
MATRIX TO TEST TRANSDUCTION MAP SEQUENCES G2)

Secqmence Codes for multiple exchange types
Goer VOLE (Operators on donor gono type )

2upoint test

123 b-
132 c
213 a
hepoint test
123h b e ac be bd
123 b d ad bd be
132) c b ab be cd
13h2 c d ad ed be
1423 a bo ab bd cd
The complete table can be
1432 d e ac ed bd generated as the permitae
tions of (ath, cd’) where —
SL3h | c be ac ad atb=bb, be, od, and bb=b,
Zu) a a be ad ac .
231 c a ab ac ed
233 d a ab ad ed
312h a b be ab ad
3201h b a ac ab ba

Tnstzuctions:

%. Write dew the donor genotype (differentiel markers only) in any arbitrary sequence,
Sales Wee at Y+ Ze .

2, Group the experimental results into the rare and frequent classeg.

3, Cede these classes as transformations of the donor genotype. The code "a" moons
"reverse the sien of the first locus written", "bo" the same for the seccnd, etc.
Thus, (ad) (WeX+¥4+Z~) would be W+X+¥+Z+.

h, The table gives the codes for the multiple exchange classes (mec) corresponding to

each sequence. Those models are excluded where frequently fourd types are included
in the mec cedes, and vice versa.

5, Tha sequence codas can be translated into maps by writing the donor genotype 4s
WX YZ and transposing accordingly, Thus, 231) would be the map XYWZ.

L23k
Yor the reciprocal transduction, superimpose the operation abcd, so that, Ccefe, ac

becomes bds c becomes abd in the mec codes.

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fable 5
The frequency of transductiong unstable
for galactose fermentation

fella dal (+) Gaye Glen Gals
Gal,- Lp* Yg/22(41) = 0/11(0) 0/29{0)

Lp* (1) 23/24(96) = 23/24(96) o/27(oy

Lp*(2) 17/24(71) - 24/ 24(100) -
Galo- Lp® 28/48(58) 63/72(88) - 64/72(89)

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Lp* (2) 16/24(67) 21/24(88) = - 22/24(92)
Gal)- Lp? 13/24(54)  0/-72(0) 21/24(88) -

Lp* 20/24(83)  0f96(0) 19/24(79) -

Lp™ 29/4%48( 60) - 18/24(67) -

 

The figures shown are the fraction of cultures unstable for
galactose fermentation. Percentages are shown in parenthesis. —

Lie.

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