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JULY 20, 1958. . terminal notes for resumption of work in the fal]

7 -
It is now clear that Hfro crosses tend to give both Lac and Lac hemizygotes.
It is probably not profitable to attempt to categorize more of the Lac” from the
1410 series; it may be worthwhile doing a time sequence experiment for the pro-,
duction of diploids, though it is reasonably clear that the occurrence of Lact
is related to the production of Lac’, while the Lac” are hemizygous, in supp ort
of the progressive entry hypothesis. But the nono¢currence of GalY (compared to
Gal® is enigmatic, and probably some more GalVY or should be looked for

However, in orddr to distinguish Gal™ hemizgous from homozygous, it will be
necessayy to use complementary Gal mutants, allowing either Gal' or Gal© to be
analysed by reversion. This will also afford the opportunity of using Gal/Gal

selection for Gal heterozygosity.

Therefore the main programs are:.

1. Time sequence on heterozygote isolations, to complete that picture. Include segre-
gation of Az, V1 and V6 as now available.

2. Gal x Gal selections of diploids a) are there any Galv; b)Ara2,4 pos.eff.

3. Complementary to 2: crosses of diploid x haploid.

4, Nay be worthwhile to look for automictic derivatives of LaeV diploids as a
Lac™ homozygote# would probably be a preferable Q-

5. Time exogenote entry in crosses of Hfr heterogenotes.

Probably first item will be the review the accumulated Gal™ stocks for identity and
complementarity to Gal,.

dn popes poe Mpladbaab Te: hep ?
Hfr

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