U.W.
MICROBIAL
GENETICS

 

 

 

 
 

ULW.
MICROBIAL
GENETICS

 

 

 

 

 

F Cake Gat

 

Cees

 

 

 

 

~
Vo tro
E> o~
o 10

 

 

 

how bO
Cae cemaal™
Cee

 

 

OM DN DMD MB Ww IN =
J

 

 

 

 

 

& ;

T_ 3220

o alg ~~

T
_— ? sist _ cuee
Po i Jo afse | pape | 8S las -
fof 339 ao TO. wfGA | 3G Ce _ -
. 4

 

 

 

~~
bee
1 1
erg |

bY f1o% P}

\* “ ne
i
|
|
|
|
be
he.
E
ies
St
. .
t
a
oO
oC
t

 

i
i
I
i
i
i
i

yO

he
Mo
‘
h.
&
K

 

?
ea

 

 

 

 

i wo BGS waren

 

DP ON Pw BP wh b
L.

f £4
18 370 _. Le... 23/ an 3S {Ss

 

 

 

 

iy goo | ajss [raise | age |
if iA $ _. —_ ais 44 a L {Xv a ee. tery phedl
iy qe L 6 SD S/48 | 3ap8e ]

 

 

 

 

 

 

 

0D DN pp WwW PP wh

 

 

 

 

 

ae

2

~ a SH
wee ALL
oe ~ SH.

6H

TH

ee Bib
_ StL.

9

 

amet |.

 

 

 

 

 

 

 

1 -
_ 2th — ae a
3 nee a
aos 4 _ -

 

 

 

 

2
i
|

bon o
T
|
|
|

 

 

 

 

 

 

 

 

 

 

 

Ryo es

fbx

po &

 

-efgo | i838

L :
ea
 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

   
   
 
 
    

 

 

 

 

 

 

 

 

 

 

 

 

 

U.w. a
5 PULSATION 1420, |
MICROBIAL
dune 11958 REF:
1 2 /3 4 5 6 7 8 93 10
. Kinetic ¢ ting have -unsatisfactory. until now
AL. recrui and entry - In Previous
2| contre , varied as approximately 1/2 power
3 of cal e), so t sub t recruitment
4\| aeeour is quite unsatisfactory.
a)
6ll In th lich may impede mating wi % preventing
7 further entry.
a ae
9]. Folla
Of
dl. Le pH mM o5 units from 4.5 to 9 Adjust D(Q) by HCl or NaOH
Ad 2. tr
2 3. versene
a 4. oglycollate
4. Se. 2
s|| 6.dead
_6l 7. Lluorouracil deoxyriboside + | not available today
2 8. 9: 58) Fl M/100 = M/1000 M/10.000
8 9. D Z .
9 ORC W-3060 and W-3064 (washed and resuspended] in cold penassay)
0 | minutes iat 37°, Dilute 1:10 into cated splutions (made up
d incubate to 60', Dilute 1:100 for! plating 1.05 ml on
118 should gave a baseline count of SRP ludéng some 10%
2ik
3 ox ded ty will be indicated |by a loss of SRP
4iinhibition of entzy will bea indicated by failure of Gal+ among SRP
5 No inhibition will be indicated by lmarked rise in SRP
g[Inhibition of mating # inh, of entry will be lindicated| by stabilization of SRP with
7 938 anfeent of Gal+,
8 .
g|| Controls needed: | Plate recombinants (plate ture after dilution) )
0 Barly interruption: plate mating at/5'; )
121000 dilution at 5'; plate at 5' 15' and 60' ) Dilute inD(>).. _..
' 1:10 dilution: plate at 5'/15' and 60! )
2
3
4
5
6
|
8 - al —
a Pullin og ‘f pansy
Ol pg, Lo ‘ i -
1 - |. oo he . - .
apt! S89 na ft, 15 spuatl wdaten pM / ) Bucttuy!
4 tate Miypalint (patel prbiighics avdinell etelind)
s é
5 ut GA.
7
8
2.
0

 
U.W.
MICROBIAL

GENETICS

 

 
 

MICROBIAL Peck poles (p08

GENETICS

a

 

 

 

 
ier aD cathe AL AN ane eda A ee Vo

 

U.w.
MICROBIAL
GENETICS

 

 

 

 

 

: | a
JOO OMN DO We wn =
———t +

 

 

 

po |
OM DN mw ke wh ee!
t —

 

 

CMUDN OMe wD |!

 

oe OY own awhp =

bo PN om ew d=

 

 

 

so

So

Co.

 

 

 

 

 

 

 

12
8.1¥

6,9
vd, rY

 

 

Aw

stroked a

 

n 6 ke

 

 

 

é ful

 
/49O C

fF 20
£ 30
P uo
se
f go

Cc 20
Cc 30e
C 4o
Cc 60

BR 320
B So
B bo

- Zac
4t t- g—
i+ i 4
it ) 7~
34 7 7-~,2W
T+ » £-
é¢, T-
“a-—
7 # , io—- (some w?)
6G # : (4-5 fw
Zt iy 9-
zi +, 34-
 

U.W.

MICROBIAL
GENETICS

Jue 1, 1459,

 

 

 

 

 

 

3

° |:

 

 

 

 

 

 

 

 

rr foot i
it~ en po |
O © DN om jw ie jw | i

+ +

 

all BL

 

 

 

COON DAD Wey nu

cop ph mua wpe!

 

 

fo bos :
OO DN mow w bp

 

peer Tw eawp =

 

 

/ is! hth

Jo. |e

ANB

 

 

 

 

 

ov V

 

Y

ray retofrer fo.
vING ceeds :

ct

Yl Ao cnrewh —» 0, |

 

 

Oer

7

nr, /¢20 D
8 9

10

 

as we

Sou

(0.0% Rie FP hte
Df)

8¢

 

fF bt OS

cp. P zu)

Wh Way

+ 1.4)

bie ont

W320 64

+ 0.9

 

 

 
UW.
MICROBIAL
GENETICS

 
U.wW.

MICROBIAL
GENETICS

 

 

 

 
ULW.

MICROBIAL
GENETICS

 

 

 

 

 

 

 
ULW.
MICROBIAL
GENETICS

 

 

 

/Y2A

 

 

 
U.W,

Periodate

MICROBIAL
GENETICS

#4,

we espe cette et EEE.

mets EE I ee

 

 

 
 

 

 
U.W.
MICROBIAL
GENETICS

Pericdate

 

 

1421-B

 

 

 
 

lw ParabeXe,

 

W424e
MICROBIAL r

 

 
 

U.W.
MICROBIAL
GENETICS

 

 

 

 

 

 

 
ra lee ay a ME ote EE ae ee

 

U.w.
MICROBIAL
GENETICS

—

J une

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

. REF:
1 2 3 4 5 6 7 9 10

; _ a _
2 Co unt Ss ccna ch Lo
‘ fer ethyl Chewrea
5 if 3 on
6 _
7 |
8 /a M9 aU
9
° .

13 ay ey
j
2 eee +
3 S| wa/ ay
4

cee _ Ss cee ;

6 | hey i
7 /
8 _ __ _ a . : an to
9 1é ! - , 8 > Mis
0

 

 

 

 

 

 

 

 

 

 

 

 

_ AL _
2 oe

jO ko DN Im in ip bw lp lo

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

5 Bo
6 _ -
IL 4 -
8 _ — _ _ _
9 _ hoe oe .
Q nao
- if - a _ _
1 4 - |
_ 2 —
3 4 pe . _
- — Al
5 _
a 6.
ft af. __. -
ee BP _
a. Ott _ _ -
——— OW

 

 

7 Mw

 

 
 

U.W.
MICROBIAL
GENETICS

 

 

 

 
 

U.W.
MICROBIAL
GENETICS

 

_ ~ pte /4r1D
beypunrte, o useage res

 

 
U.W.
MICROBIAL
GENETICS

13

 

 

 

10

 

 

 

 

 

\FFEREWTIAL

 

 

 

 

 

 

 

i
Oo} MN im kn la lw ip j=

 

 

 

 

 

 

 

 

Oh} DN mh Pe wD be

 

 

 

 

 

 

 

OC WRN Om hb ww |

 

 

 

 

Peo my oO ln ie we De
~~ | bog po,

 

 

pb DN on ew b=
n°
on

 

 

 

 

 

 

 VARtouS) ACES
fo Agi o

 

WwW toty

 

 

 

 

Le Ab Lead te"

Dh

 

ea

 

 

 

ts

 
ee ne en Cg de eB Kalb eM ee eh EE a Ds Ce eg ede Be —_
: Mins ia 1 ll, ae 3h te. .

 

 

U.w.
MICROBIAL
GENETICS

 

 

 

 

 

 

 

 

 

 

a mw IN i

|

 

 

 

 

 

 

 

|
oO WON

 

 

 

 

 

 

 

 

|
|
: | i :
obKD DPN mM mM fF wHN
t +

 

 

 

 

__ ‘A

2
all. -

a . Al

5

6

Ae

BI

_ aft
0. 5

 

 

po mn Ow la whe

 

 

oO ON Oh ew De

 

 

 

 

 

on

 

 

 

302
3 ¥9

387

 

fom By)

wh od

ROE

 

Le f .
bay

iz

is?

ve

ye 4

 

 

Heh fo

wh bol.

- DCI4 &,)

STA
v4

Yo 3 -0or D,

“4

Sas
A, 58

 

Sun 8,

 
U.W.
MICROBIAL
GENETICS

REF:

1423 A

 

 

 

 

 

 

 

9 10

 

 

 

 

 

 

i |

| i
Oo DN Db we w iN im
T T rt

 

 

 

eRe
al AL?
ait.
_ 3] _
_ __ 4 6. woe
—.. § .
_ 6 ; .
_ al dw
_ ne ABH on
ae DT
oll D.
2 we
By a
4. .
5 a .
AUP
2. .
oo BY
9 £.
0. .

 

!
OW DO me wp o !

 

COON Mn py Dp

 

 

 

 

 

 

 

 

 

 

 

preted *

Cornet) .

‘Ave C90 .

woke « C2-&s

 

 
 

U.W.
MICROBIAL
GENETICS