14.05-IL / DATE: Me. 24 REF: PRoTert. 7 | 2 3 4 | 5 6 7 8 9 190 12. to 3060, Jobe wht, > | AIO wd | abt. 2 &y Net Sa toa as jer : a. f 13.20 luk + FY Lo 4 fo" ponce. “> pre 3060". {[5-To Rewdi pose , pty rj{prarnd pools pate nr LU nude ein 3 Atje 14.50 Jobo / 864, 3 ~, 1 | is.s9 (sXus/,, reYuguand wy oe Zoho Sedo Joe. So6 ~~, te Ke re — us taf 2 sal ae NE 4 anny ¥ , “ree as Poem. 1% ) oo _3F e a 7 CLF :| OS + G5 wk wale, j {ml 2 poured. comiahes ie kad 16.4% A, 8, DE Zo! witatr — OS 4 UT wt wake —7 nein St B, JL DP OS aT nt frwske)yt —y Ofer [ua Co’ de > Ay. “t / ve OT we. hy dan aI pop 2“1}6 0.05 uf foam (0% 40) added — Mi cro svope co Es 3060 420 x10 Jk 3064 |, Feo xlo!/ume 30Ge jnshe at 5 ad ele / wt ~ © reds. 3064 n 2.7 jo ork "4 8060 pw a: ayfk preted Anke f, ah wal, piphey “p ond aloet y mynd ~ DATE: 3/2 4/5? _ REF: h4o well TT L 30” o’ ( | Exp. A IS | 33 Sy ee | : B ° o o | ° ! ' ‘o D o o | fo | E | o ' / , Powrela Ofom potty fom c ¢ 30 fo) 278. t (3 o6G ) : 7 tt. 20) Cpuelugry : Joo Lu, etn te Ft, Te fare fringe ots (fete ba rey ofa Og yp. (403 Heng 30), 40 50: 3o6o : JobY : te hectepter Prepovetnn ¢ ‘ | 3 | 4 | 5 7 r 7 8 - “| 10 po - pesranntty oy 7 - Exp (Mor rT & Yep ead ed, but aftr | burg E nel Len Dw, | 9.057 am. / nt s060 oveeuighe otreh + Gud bre7t, -? Rot | . | 10.370 1 wt 3060 expen. + tat L, far Coowse prnnlnr greet. AG RTA, 10 Pon Hpk Ak fo : {¥, to 306% tol + tow Grote uy ! i ee ae woke Gott, 3060 @ fox ——rd.1 nh + 09 an On om 8 Lac, ~~ if te ‘ nate) 3064 jox ——Ta5 Nae ane owed > —_ 2 | | 3060 6 soy resus prmadssle im ew L,.: s tox | re gt pen te 3% (Op l. f Joo e) nideTed un Wwalie for ave 49" than: libuted 20% witt Ll. wradian wrewmed o& Oo’; tuot + Frye Dw —y 1t.0 0.06 avs 0,0 om ein lt By | : y | [ | elo wl bute, wollte both (+ 0/68) — ptite fo. 05 wo’ a ’ — + 10/50 jo 7 . | ug! 4 % Co’ 7 Vie DW —> -X- Co (irt- eoTT 0.07) 4 toe tri, —y ~ 69/0 (72. 0-997, 0.01) 1 2 [ 3 7 | “5 - 6 | 7 | 8 ‘9 - 10 Bo Coe of weds tlle ce poten mein | | )O! +.9.9DW, —yO0.) Ems bme plot. 2bb6 cok. 10) ( Farw. Jems oy Ah Gor & Le, ~y °.! E Mb lee 4 ewok, | L | _ | 7 teens we 6. a ~~ (dried up) 60! n > * FJ cok. 20 r | a tint Mo, of cath, which were proGyptastid 4 nn lene wie. bur 9 g te okt 2-5 to Amd. Hernce f-Zn. fore % of te ordie 107%, ! | : | a) leo te 3D 4 >> Ge Lat ites | 30) X- 0 K-19 XX" XO KH & Feo 0.08 0 191 oo eo os | 0 sof 0 41 362, Goo Mf90 = 760 | 0[ be 10] 92 10 | Uo veise WF bose 8.051 44 178 De ) wo | co ; o at yy 4 iz ¥ \Jo0 = 609 wha oF} ay NJoo | ¢ Hote te KL | | the Eve Knew oe Aaa zo/4e 30/30 ysis 60) o/so e/ So [Fo i/s0 | fs §/se 60/0 [2/50 oo! K-10 X- Vo X¥+30 | aC x-bo. gts — 0/40 o/s of 50 ute o/ka but | o/9o /so Use | q{se (0/50 qT 4/40 27/5 14/$0 24/50 19 /5Q 30) 2 4 Poe 7 ft trom pratetens | 19.30 am, 4 wat S80 3964 ~ fo tel, La, i 4a. Fe pes poorly few, ohne | sob¥ nell prdwe ; Z- Seo +10 C/o. Pare to* Wah added, 12.35 nbn of pote, polt rietad C4 Gack bolt {S00 5060, 3o¢s alr, creed : gefo, 2064 protien s Cavte: ey mL 3064 | 3064 2 to wt J2607 Sut "1823S ee 7 . : (Jobe Se. | $e: A C D | E (30f0 | 0.5 ose ~ 2o¢0 4 aa - Of OS | Jott g ~ OF os 30) o¢yY inl oT — OS | ~ wel ° Ate i | | ! | we 0.05 Ble Vie beet ¢C: 3060 o Ol mw + 0.9 wk e804. Beg Vest | Ee 3°S4 6 " a ‘ 16.0. foe virakey Aw A, &, yn, €: _ Vie pw nae OR + OF wt wee TOS mts, '¢ A bre ow or jrorv/e : A, B, E | 0.085 fem bet Eb gr Ufiw ati te 2 @.05- Vive aew ly »D : o, os form wT ate, | | | ‘. 30°: 40 50 yarn 3% 9 foe an ° rd bP ay 7 ee 6 3 SK’ \% -t- 9 ° et a t Oo | care: 4/14/58 ee NYO Y : Surrurery of fprpahiry $ sm & Sere _h, | F "Gels —— Git) Tr “gqet+) twee TS —~ - A. oso g/so | vy/50 U)S0, V2/ fo" 1 - 8) 8 Soe 10/50 | 27/5 abe - . eg 2 DO ; - v4 /o? rt/rd oo - EE 2/\oe [3/loe S8foo 0 /S% | 4/50 16/50 ! ~ Absa : a od nei ied Frm D : Streekg m EME lac. ‘ 2» bali, : 3060 ghorrad srtee; Hy pr fo, rhs . | pectin J 50 | | | 1 | PRoTOFLAST CRoffEl . | Ryton of iWos-4, 3060 30k4 BS og ial Upeuet 1409- ¢, tur aot Se wit Z,. J06O Jody rods c., 2x Crmeertahid fran ‘0 0 veri hy potaTad eekly, | 3 | Platt om ie Suc G6, St B, &St M s S’ A For 0,02 4 O.er 4 008. 7, 09k N 6 emt Avo 0.02 | Joo 207 Hon 2% i. oor oO Howe |S Yo Pes ! Y 00s Yo Yves so ?P New. Nenu. Ye 0.02 ! — Hoo 002 % 0.02 Moxey, ro fg ST op ln ty vad Onno Git [2 20, Afr 60" auorbtr a wate te Ar, (HK i) ond pot a CUM Yh om tor | Ae, oh pfo: Fo wt 40 06g (fh fry qT rr, { oy weds y i | Jesy gi. 2060 a Vere ww toaepat ion ] _REF: [¢o v- - DATE: _ . | 1 2 3 | 4 5 6 . 7 8 9 Se Seeise B. sue Ole win G, wie Se 8, a m» 0.02 s/h tern | oot: wv £09 O.0o. many 9.0%: weeny |, ae au hence >| AJ2 | ona 158 wt. + Amn peather 4 to eaters. a g wn (@) 30/2 374 25 31% Now 0 (0) lof ? {O JIG 9° fv Soe | Now Note (P) wv Jo0l0 nN Jouo & tooo, Covbty: 30002 — rant yma Gar ! lv 2eee), | 306 2 y~* wit. ye gk se Ope a eteely Picks | ic Ramey ; we . | | | — = . 3 [ 4 i 5 - 6 vo 7 - ‘a 7 o a ; oe fi + A _. a of Pp VOR-3 fe det Lae heme can til ed en iment ph ing on Lo-< ot jo’ ( See GesGiry wevte lan preuiouy yee ). 1. joge é, rer enmdid in q [0 xX cane, / form wih Po fo ovy tft retet ca. To £ eben I~ egal uw, | Ase, vied: OG tet of Jobo o D Yo Poe~z m EME Lae, (465 wonies> counted), | Fo 39602 x Jol , 10° phe Pham A440. 1100 om cl Pitter Lo kt Pott tein in 6. for UE TOI! Ale fod Tomer ALD), im Dw Te/o 7 (en eben fv lo, toa ahd: Cyr. q /e@ nwZ Promotion 2% ans : | A) immediate plating | | B) Pact talerded rn bm kl yh, Y 69" fen a0 end of prtte, , tem perry, ! Plax 2's : Ol, & Q0e m wu ft @, : By vairheme ) grey bes Lew. At at rooms Weare role re. | | 2g" $0" 3s) B A | a A B a0 NoW iW We, | Mew He) NOL | Mew we, ime, Out ry 88 I9¢ 146) 26 zz 0.02 23 10 27 | +6 35) | DATE: 23/¢/S8 . REF: [405-6 - 1 Yo 3 4 _ 5 6 - 8 9 10 SEGREGATION, . . / 2 / Mow ‘NC, Won | NC MON (Ve | geht cfioo Ofice o/ioo ofios o/19 0 ofoc 10) Lac 2/ic0 Soo Sivoo A100 7/120 8/944 Ti 25fioo 38/94 33, 37/1089 3g foo 54/09 _ | | 30 40): 50 — = i REF: - - 1 3 4 5 6 7 8 9 10 CROSSES 38 xk 3. | é yo. 2323 ,o6¥ pos Geto re “30 vy col, (tome + G mt from Oo. 2c.) Ragged an b. pediuwm at ! do X done, ° eX Ma ed equal aon andl, Phen HOTA Ao off. 10" pelpe te Ly 1 ° wnedicn em bwecrteted 50", thee Spread. vy main j 4 Sag refle Gb : : and VYig- gure 0.05 fer unt @. a he, Korb ) Awd Aj ilenrp ae fC. Hee din my | Pro portal SSP VS I —? ho Ayt. weter 7.05 ‘8 fre | a i Sy L, 9.08 on fee 8, | Lt 20 | hoo Ly | Y Uv, _ OG fuica/e Fecal facg | Yo by a Jarre. end witpo/repice | counk . . . 42 x 1oI f Micvefop, comdp :| 2323 6: | je ZSosoH @& . F2x 10 fue al | | [ oF nae ' : 3964 \o7 | ra 2-10 [root viodds U3 320. lo = 6-10" /at wot Vie Ce hey : So6y v lo. 000 40 wy oJ 3600 | Cros ny Coke LIB Deane 3064 pardon pal Cdgcy . sal Crosses: af eiwt 3obu, ! (go fra. whe w . | fo r “ { ° to D ihe ' oATe: ASIS ———-curtere | PHASE, oer: FOS | 1 2 3 4 5 on 6 7 | 8 I 10 a ae ee ee te oo fe __- Peat mate tse mh D eum 3060, 4 wt £1G unk bake (0.29 | 70 CY. (art | 0.27— | / o S: % 0, 3 ’ | Ou P. uw, | lo aw. neh te | poe nadie ~ nm. 10 Ayo 2 Slee Ted over asested ) broth tr. ge6e Sar OSt : | 306% Siz, 0.80. redneedy ! Ath bt Tome (to dainty sot be teat 2060 capri sol hey Abt wh Onfh. (2984 POT tos wo, LOB nk + tmeg 3060 Sor FS mts Gwe tre , 36Y Sot Pat nis Km)_ NM:y Rave mw tynnk anno wwG ) ee +. Ln fo WT aba7T : 3066 ser 3060 Exp, 3064 for 0b Exp. : A x | x | | B x x | | co * x | 30 D x ! Ly 5 WY a tue | pervaed Sth, to 09.05 wu JG, reeks. v —pa.0r i” “DYN 5 og, 01 dye for A, care. 4/17/58. | per 6 rr re 2 3 | oa 8 6 8 9 Ate do’ | A B d d- 0.05 482 243 492) 320. J * “f Ys te. lay deve (py weo | WV 1ev0 V0 00 ! After Go! pr ‘s Ct AEs . | ojos weeny frmcranly Jwrtarg) wat] 20 Wo 0-05 498) 3e0 | vSoe Te | 0.07 | wet dn >> 59 Gi a) yo 50 ore wLTURE Phaje UIFSE nee, (IFOT SUMMARY A Ls) c D x j J exp Ap eg 9 ORD tp [4 Oo | Mot o/5o ffs | %%> | °/Se Gol $ 0/50 0/50 o/s° Offre sarc 4 '/So So Ys5e| o/Se 20); [2/s0 gjso 10/se Hg / 60’ Mak tt o/To : ols a/seo | o/se Sake 6/s° 5750 2)§ 9 6/50 Love vofro 16/50 12)s0 &Uu/s2 30! tT 30/s0 3e /s0 42/50 2G /$ 0 50 10 20 30), ao ah A € * Fj ". st phage Lin Lon via As h. phage pas ie ile may result; in a bransdud sional eyant., | igteregenobtic clonas when donor and recipient boa ve adcuun tive recons, The hsterogenotes were uns table, the two input and two crossover classes (dozse : and Ledarbsyg 1956). Two cistrons (cis-teans oad iedon offen’ groups) had been demonstrated: trans heterogenotes wate name vers of the save clatron are phenotypically iatant. Caleeto. Hinase was migsing in mutants of ons cistron (Gal 2,8) while UDP transfexass aati. ty wag absent in the other (Gal L; 4s 5, 7),.8 dsfect corresponiing to haman congenital. galac ton semia, (Kalekar, Kuvahasht 1957), Gal ard Gablg™ azo : gonetlically clatronic with both of the” fo: eget Ng groupe | sy have not yet been successfully a madysed for the: aatie defect. aAgeng two undred naw mutants, no ree ident ical. witha the first tan have resurred. Most he. aasignsd to aither of the firsts two elstecnic groups, Li numbar which would rep resent a fourth ganctic @ given only normal galectasea=positive ks wee tested, A fer mitunts ware nob ey other podit fers. avery standard Gal formable by f but ware found to co. vlnding that of hexoses ms tabolian which cbscure their lonship te ths Gal” groupee-=/in athempt vas made t 3 markers tn Linoar sequence by their rola thong nip Locus, Jatarorosaes of Hfr HW” Gal.” x F° Gal.” wore a fy * dthe ‘a oh ono medium which selected efficiently f combinante, and th:sa were then scored oP an JB marker. H weve the are LOth Internat | Consreas of Genetica, Montreal, Loss. U.W. MICROBIAL GENETICS oU WON DH SF wD = + oO ON OW PWD & ow ON DM bw HD oo ODN AWM PWD oOo WMW ON MDM BP wD = W 390% W 3870 Gy UF t. imtiog Se 1.0 f _ brrerbnbinde pr or PLEA om Phat for G, fy FOS os Yow Carter : oo of md fid enrllorey, wot | wdc l 7 lope Gol + & Gol - Mio $6 Gol+ | qal- Hie0 4 got + 3 gol - Mowe o Co-wtwet W3Io08 3 eek. W 3870 © uk. 4 é fe, How » OVO U.W. obo ON OO hw HD = wma — o May 19 1958 a 1408/3 — a hy Linkage ‘(| @ | 3908S mete lp”), | Ga: 1 Ww 3367 yk mR Mer‘: 5 2A. W306o + . th? | 28, W3t7o Ak - 1h? (A Gat tap 6 im : 3. W3848 si Paty S G4 Jr $.. ‘ 4: W37s2 s | Croy | ; 13): W3782 s M- | oc & +99, 1 39 3 ZI: w 38q8 +; C- 4G A), of of fo” 10, bo? pda ioe to role ne ty é | ' ia? ° | Gehl Ip /gat! — O6'/Guk 2] 4. oO |D ®@ _\|~ | | ‘| 2A oo 5% 70 32% | 26 | a 257 a% 1%. s| 3 ly oy 1B 7, | 3 \. 29 2 16°, | | 2 3 26 es oo yr | Pe 7 ot yam 6 | care. SIS SJ oo te oe <7 ¢ ce t g | NS h { h eg end, ! peel me S Gk fn 8, D( dn GJ Mm Gat prs & Tryp a od be fore et blew peey (& Se $ & e, eka peach em ft mee vs S AS ep 40 Px f x . & WN * oS 20 i8 ~ Tt ah bo | So 3i a1 (TY 149 5 f 40 | Ivo 1 48 Sy gL GR 50 Ga eq fo (ism) ~ . 4 4 pon Bote f Tr LAD “4 7 ot ‘ V yale . s Ly taf * aa pudt. te tend fe Bo fu ee 4of.y po ud UW. MICROBIAL. GENETICS ee 19 REF ito Whee 3 4 Ss 6 | 7 8 | 3 10 2 ¢ GLkf-B, | Corrie] _ 3 aL. a f . . 4 me . ; 5 wt a A . & ; 4 7 qabtltet] Tat qnkey, |oatefeer tot erg By ws & ~~ yo - i 0 to! iffe, 4 240 1h aM Nag mall I, . - fo G4 jr. pasa ain 17/94 WE |) 2h, ee Sey 2 - , ; : ; ; / "EN gy : Jo) fo far | 31 Of, | S/H 149 | 3.3% Bo NO a pF ‘ * ee .t a 7 5 40 8/94 | ur 16%, lid/400 5% |ae% qo 4 7 8 . Vigna 7 so" Y7/27) 423 hry weaany a . 2 i cee ee . i a bo! . tos. tebe : vt . | eo . Tih a 2 ai 4a _S 6 Zit 8 3 0 1 _ 2i 3 4 5 6 7 8 9 fowl ; ! 2 3 : : 4 : s ! : | | | 8 | 9 | | 0 | t U.W. MICROBIAL | GENETICS ° bed tt .. -f Pop on fone . 19 May (6 i! REF [<2 LA a = : 7 ee 1 2 3 4 5 6 7 8 9 | 10 { , a VY eee . web . : . . bo i all WW 18 pe] me, aba [DP ew Att) = I St oe a - be - All We 34.48 “f A} wet 4 | 7 —— 2 wet: 6 — _ a dpe, reguespe dak | Wwrredl MaIn4 na x Cte 9 . . | . . wa . : 0 2ei Pi + tone ¢ t+ Code Ll “4 4to4 te. i t - A | Co uh SB dekle ce ee Ah 5 thee es al, Co us . © | mG eo ee ~ tpt 8 OS nat bse > al To ‘ - 5 2.08 4 64 phePed - 7 Vee ; v nd +, Sy ned Lr /t ~—F t- f y wv oe “> cs ft - BI + a 9 pee gene whe ge . _ _ ail . on hy YELS ats _$ . Os] gh Cuted = 4 - C J eA Caped ze t Og oy lave . Per Go 4 . . a a 4 al feb | firn prety ae ep dy mteas of Yrorwted ge a Y _ 6 dink. pipe & LS pnt ca Chad eee tm, toh LA af amd | ays} frit. Pb ib Oo)” ptoTed. oh 9 | 0 LD fe 3B, Meth) ; (Gk fx A, Auep 2 . 3 . * a - 7 r f on * ° f of ” aN . 4 Tined fe, Qs ve lar! 24% 26° Ld) fo ze $5 'Jal Gfiwe ¢ — 7 / oo ° f j ft , 7 . . v SI 6 yo fo 7 ‘ a ne . 1, yt / a Vole (BU ached, ST 70" Qo TT - a £ : . # 4 3 7 ZS o vy Je ) - 1 . - 4 { ae be | | - 3 ~~ vf. , 4 So 0’ 35" 5 7 a ae 7 —_ . +4, at | ; | oy y 3 tow 4 woh Lins Pode ( een Hy oath if ") , Com tacqeane oan pron 9 det HM tf + - or ; 0 Wea tino : “vy Fleas Ake - Ex p , dis¢ pled, . ULW. MICROBIAL GENETICS i____ _4 19 iC 193 f REF l4Lo /s. _ { / 1 2 3 4 5 6 7 38 9 10 GAL PREUM p (AMIN G Pia Te. wee a > to ote anh ny 4 fe ervd SIS y SGoh _ : 3; fjorr Yom “p> 1VO8)s - Cuthrs AorAa nwA nl A. x ‘le 5 7 : i, Lr 2» Pre We ; . Cot fegoal , Have 4 de. ke wd ( ) kh Ka 9 f~4 . ¢ nL a5. -t f une 2K, ! ° fees ot to! at yok, Ba thed thndsd, akties (of if 1; A ! , MA hot, z) Goat gfr 4 D> 4 5 Comps _ of 4 Sg OF of ? 6 5 8 9 0 1 2 3 4 Ss 6 7 8 9 0 1 2 3 4 5 6 7 8 9 o oo ON Oo MO Pw Dn =