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TIMED MATING, Hfr, and Hfr

1 2

ORC Cultures of W 3936 (Hfr,), W 3948, (Hfr,) W 3052.

Spun, resuspended in equal amount of Penassay, and mating mixtures prepared
in flasks with 1,5 ml ¢ and 6 ml ? and 2 ml fresh broth, Samplings: 0.2 ml at
every time, dilution in 9.9 ml chilled water, .0h spread on plated (A) and the
same for dilution (B) of 1/10 for Hfr, and 1/50 for Hfr

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