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TIMED MATING, Hfr, and Hfr
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DATE: 1 t/s8. Ge rer $403-T
1 3 | a | i “| 6 7 8 9 / 10,
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DATE: 7 REF: Igo 3/4.
STABILITY oF PAIAS TO - PLATING,
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GENETICS
26 Mas IO REF:
1 2 3 4 5 6 7 8 9 10
ited v= =f 740 = } *Y, to BoF4 vow 3S. LL hac *S oUlgr ta
At fe wae 4910'a0 felt oF” Phd pope pgenrtecs
Oo f& WON
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DATE: UY arch. 25, CALA
rerPRoT opt. TIMING
1 2 3 4 5 6 7 8 9 10
9.30 | #3060 w+ St bk 2 whl.
So | he ¢ Pw C, ned
‘re lo? A fpen. fn’ .
1G.te proG nr Tha hifrar uery, c7)oth.
10
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W Or ws tot.
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5064 tn Dy nrecliam
20 ee —FT
runt B / [we ie |
6-10 nb 4 tt Ye,
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|
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: 3% or 4s; ¢ ¥
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3064 on byw. 410° / nb
Pore PLAST
oer REF: (qos t-
mr Ainf B, ’ 5’) le' Ve’ 45's fe' | Me’ 0 | 0 |
oe P Gad
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Ko Cross 2 2 3 30 89 He oe
ae °o 1 oO i to tt 32
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Reconbinadly fie Zpebaplt oft 60's 2.3 tol /rmt ot 4 Flo = 310%
Not . | oft peaemeneky te OF cont |e inte
7 pooteptotty. |
At tie Ve! s) pm pepe Ye. 13% (Hr por | Ree reed"
Yo’ 10. YH rm
60! 1g. Fe
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7 Thy geo for tthe A Gal aby ooveg
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14.05-IL
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DATE: Me. 24 REF: PRoTert. 7
| 2 3 4 | 5 6 7 8 9 190
12. to 3060, Jobe wht, > | AIO wd | abt.
2 &y Net Sa toa as jer : a. f
13.20 luk + FY Lo 4 fo" ponce. “> pre 3060".
{[5-To Rewdi pose , pty rj{prarnd pools pate nr LU nude ein
3 Atje 14.50 Jobo / 864, 3 ~,
1 |
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JL DP OS aT nt frwske)yt —y Ofer [ua
Co’ de > Ay. “t / ve
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40) added —
Mi cro svope co Es
3060 420 x10 Jk
3064 |, Feo xlo!/ume
30Ge jnshe at 5 ad ele / wt ~ © reds.
3064 n 2.7 jo ork "4
8060 pw a: ayfk preted Anke f, ah
wal, piphey “p ond aloet y mynd ~
DATE: 3/2 4/5? _ REF: h4o well
TT
L 30” o’ ( |
Exp. A IS | 33 Sy ee | :
B ° o o | ° ! '
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t (3 o6G ) : 7 tt.
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30),
40
50:
3o6o :
JobY :
te hectepter Prepovetnn ¢ ‘
| 3 | 4 | 5 7 r 7 8 - “| 10
po - pesranntty oy 7 -
Exp (Mor rT & Yep ead ed, but aftr | burg E nel Len Dw,
| 9.057 am. / nt s060 oveeuighe otreh + Gud bre7t, -? Rot
| .
| 10.370 1 wt 3060 expen. + tat L,
far Coowse prnnlnr greet. AG RTA, 10 Pon Hpk
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uy
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>
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re gt pen te
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ave
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| : y |
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wo’ a ’ — + 10/50
jo 7 .
| ug! 4 %
Co’ 7 Vie DW —> -X- Co (irt- eoTT 0.07)
4 toe tri, —y ~ 69/0 (72. 0-997, 0.01)
1 2 [ 3 7 | “5 - 6 | 7 | 8 ‘9 - 10
Bo Coe of weds tlle ce poten mein
|
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10)
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L | _ |
7 teens we 6. a ~~ (dried up)
60! n > * FJ cok.
20 r |
a tint Mo, of cath, which were proGyptastid 4 nn lene wie.
bur 9 g te okt 2-5 to Amd. Hernce f-Zn. fore %
of te ordie 107%, ! | : |
a) leo te 3D 4 >> Ge Lat ites
|
30) X- 0 K-19 XX" XO KH & Feo
0.08 0 191 oo eo os | 0
sof 0 41 362, Goo Mf90 = 760
| 0[ be 10] 92 10 | Uo veise WF bose
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SUMMARY
A Ls) c D
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phage Lin Lon via As
h.
phage pas ie ile may result; in a bransdud sional eyant., |
igteregenobtic clonas when donor and recipient boa ve adcuun
tive recons, The hsterogenotes were uns table,
the two input and two crossover classes (dozse :
and Ledarbsyg 1956). Two cistrons (cis-teans oad iedon offen’
groups) had been demonstrated: trans heterogenotes wate name
vers of the save clatron are phenotypically iatant. Caleeto.
Hinase was migsing in mutants of ons cistron (Gal 2,8) while
UDP transfexass aati. ty wag absent in the other (Gal L; 4s
5, 7),.8 dsfect corresponiing to haman congenital. galac ton
semia, (Kalekar, Kuvahasht 1957), Gal ard Gablg™ azo :
gonetlically clatronic with both of the” fo: eget Ng groupe |
sy have not yet been successfully a madysed for the:
aatie defect. aAgeng two undred naw mutants, no ree
ident ical. witha the first tan have resurred. Most he.
aasignsd to aither of the firsts two elstecnic groups,
Li numbar which would rep resent a fourth ganctic
@ given only normal galectasea=positive ks wee
tested, A fer mitunts ware nob
ey other podit fers.
avery standard Gal
formable by f but ware found to co.
vlnding that of hexoses ms tabolian which cbscure their
lonship te ths Gal” groupee-=/in athempt vas made t
3 markers tn Linoar sequence by their rola thong nip
Locus, Jatarorosaes of Hfr HW” Gal.” x F° Gal.” wore
a
fy *
dthe
‘a
oh
ono medium which selected efficiently f
combinante, and th:sa were then scored
oP an JB marker. H weve the are
LOth Internat | Consreas of Genetica, Montreal, Loss.
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MICROBIAL
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MICROBIAL
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MICROBIAL
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1 2 /3 4 5 6 7 8 93 10
. Kinetic ¢ ting have -unsatisfactory. until now
AL. recrui and entry - In Previous
2| contre , varied as approximately 1/2 power
3 of cal e), so t sub t recruitment
4\| aeeour is quite unsatisfactory.
a)
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7 further entry.
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0 | minutes iat 37°, Dilute 1:10 into cated splutions (made up
d incubate to 60', Dilute 1:100 for! plating 1.05 ml on
118 should gave a baseline count of SRP ludéng some 10%
2ik
3 ox ded ty will be indicated |by a loss of SRP
4iinhibition of entzy will bea indicated by failure of Gal+ among SRP
5 No inhibition will be indicated by lmarked rise in SRP
g[Inhibition of mating # inh, of entry will be lindicated| by stabilization of SRP with
7 938 anfeent of Gal+,
8 .
g|| Controls needed: | Plate recombinants (plate ture after dilution) )
0 Barly interruption: plate mating at/5'; )
121000 dilution at 5'; plate at 5' 15' and 60' ) Dilute inD(>).. _..
' 1:10 dilution: plate at 5'/15' and 60! )
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The Moris wer alee ted up wut
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MICROBIAL Piha ag f ial page l
GENETICS
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(>, 3)
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Phare fou pusut dylrbe
7 /] F Beyamarn Ltld, sgl Bhaord F3i/.
| fea eis FS.F)2 $10, 3,13 £128
benny A fre 24; CR, FAL, FRA F23 27, W Bo, . 21, 24
be fr daeloage F37, 38. Lauf30
0 Letan dn bee FR alert © ese e secede .
he ptt baa fe Ne
be Aegon fh tick ei
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GENETICS
ama od anda nil \" 00 ol AVM T AMamMmonmadama Amid
| > jo: ae pk Em
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wb? wyoe wobr
Hy, Aer Any Bay
JULY 20, 1958. . terminal notes for resumption of work in the fal]
7 -
It is now clear that Hfro crosses tend to give both Lac and Lac hemizygotes.
It is probably not profitable to attempt to categorize more of the Lac” from the
1410 series; it may be worthwhile doing a time sequence experiment for the pro-,
duction of diploids, though it is reasonably clear that the occurrence of Lact
is related to the production of Lac’, while the Lac” are hemizygous, in supp ort
of the progressive entry hypothesis. But the nono¢currence of GalY (compared to
Gal® is enigmatic, and probably some more GalVY or should be looked for
However, in orddr to distinguish Gal™ hemizgous from homozygous, it will be
necessayy to use complementary Gal mutants, allowing either Gal' or Gal© to be
analysed by reversion. This will also afford the opportunity of using Gal/Gal
selection for Gal heterozygosity.
Therefore the main programs are:.
1. Time sequence on heterozygote isolations, to complete that picture. Include segre-
gation of Az, V1 and V6 as now available.
2. Gal x Gal selections of diploids a) are there any Galv; b)Ara2,4 pos.eff.
3. Complementary to 2: crosses of diploid x haploid.
4, Nay be worthwhile to look for automictic derivatives of LaeV diploids as a
Lac™ homozygote# would probably be a preferable Q-
5. Time exogenote entry in crosses of Hfr heterogenotes.
Probably first item will be the review the accumulated Gal™ stocks for identity and
complementarity to Gal,.
dn popes poe Mpladbaab Te: hep ?
Hfr
MICROBhae———_ £° _____ .
AOETIES,
sfelst
ce GH NE V Coagee setts V ond ~ ( west ) Ae yey Jom — _ “tT
a ven dp a Peery ) al _ wee ~ arto
an ay 42 uy Lm Cand weak oe a raen| -~ oUt - +
of TLV - - ; - + - —
: - ~ 4 7 eal ta \ : possibly “pev + ae _ —
spate UL VOM beh ee Eee
pel PR Es
ao A ce eatin . . - . —
5 - i tp _ \ ale ssilely po : _ —
~— ° | a4 ll (> a. highly ppt iy from map + re
7 3P |] V — (ard V7) ft — -~ >
8 pe —
a 9G 7 ST a = ysl (er Vv? — — ~ +
erie | |= osteig lee ¥2)
J / O | = sv weak _ — - .
u Verde hom me] —Caezti wi] -
an ‘ f " . we Per ( alse ta tin: tel)
ate _ (nage ol ph AY]
Vand) og Kem pop + + = ~
— | Vad ~ | pos hl ov Sen J ~ celen, duce, | —
and. | poss hy vam | Besley types
U.W. ——
MICROBIAL Eso
GENETICS o,
Dee. 1 199 Sf REF:
1 2 3 4 5 6 7 8 9 10
a eel q any «Lae lhe
Are Lae Gah } Ay! 7
- - Bil Vv Lv od Sl (weak) orev | oo ~— AKs t's from
ec a ge TT nn pacer ETAT SSE eae - “ee ey . _ 4+ S possibly not
—Cehov LM enh PS pele a te t- 4 — but simply
OTT eee oH poten ; Ln iktel -> festreadted -
. “3 \ee la . ~ _
__ gah te Y. fm) highly Pe " impae + | proved full +t.
a 13H ve - - — -~—
v -
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all yr 3sll VGRJ — ~ b —
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bay Se 3 ands v?( CP Eee ep
Ves, ? x | _
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cn Na
ol - s “wv i|-/ 5\+ pao: ater Udey 5 tT. t+ ” _
wl ev [ess ppt | ot fe fe
__. APPOAL 4A
V —_— — _— — —
“mtse dl 4
a “pe | . - sl pap Bi treed = - - ~
oe ey : cate .
Vs LV LHD sughe ate yds t+ - -
V
V
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MICROBIAL
GENETICS
REF:
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MICROBIAL E So
GENETICS
= Farents on Sugars
U.W.
MICROBIAL Peo y
GENETICS o-)
, 3 4 s 6 7 8 9 10
" Fagillated areas From lac- colonies 3 day s vld were strar ked on
2
3] B® hak +e lool fer V vis a straight tr.
4
5
6 a
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MICROBIAL “4
GENETICS
Dec. 319 FF REF
1 2 3 4 5 6 7 8 93 10
1 Lrterest centers in Galt Lak- pag lating: 7» SO of fle questionable
2
3 vis | from ses EO A ; Severn) Suspect coldnces were restrenkedl |
4
5 ow Wac.
6 bac
71
8 [- 47 |
9 rr wv {
0 Nenegnaen gi egilinae a emeennanpeneser deacons — 1 PE probably PtP
t
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2 - |
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7 Gel (5-27? Vv
8
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2 ye —
3
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6
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BLP
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1
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3
al 4 - + 4
5 , i i x
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7 \ :
8
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1
2 S- t} Vv = ry
a \
4
5
6
7
8
9
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ULW.
MICROBIAL , FE so >
GENETICS
Lac Reactrons of
Origine Eso’s.
Dec. & wp 5S REF: ee
1 2 3 4 5 6 7 3 3 10
o Streaks on 8 Lac From DM -Gre- B, liquid of origine|
2
31 € Soj's ¢ only fhose circled in | red by J 24 i
4
1 DAY (ane except : \ a DAYS
7 op or PP?
8 /- (4 very few dark > Va V jp orlpinet darks
9 now flopk falf F
9 :
a- (34 | al — | bluish e/pinkish |— ‘5 Langer
1 | col gt p> late . Few Fevg
2 im ~brush = area. VES
3 B- Vs , very few kK Baayen bil pop
4 ov iain darks abe look fall ty
5 Lia of west paiilatd Et.
6
? be § very Sew dack > 4a rer a a (pert bly v7?)
8 ory: darth seem Fault
9
0 s- 5 _— —
1
2 s- op — _
3
4 i
5
6
7
a} F sO Flom pit & of above, pic\ add streak om Lat and Gra:
9
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7 "4 or f* . i '
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2 .
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4) Ty pi ing Hr bacl's ot AC * etsy ould tr
5
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; CF Yir
9 le, Z ’ , ~
Q 2 a ] —— at whe & cee Goa yt Cina: or + + pn / ~ Le et we.
Pi t
1 oy
Meee 54 FO TL Ih P| Lees Xyla Caka Houaa Yr Ve S
3 tu . a" f - oy He
all wo be Hir yg Cray | Lage Th, | 4.24
s #
6 rp a aan t —] “ —
Hee pe hag 1, Jrfe ~ 32¢ HO fe Sa ee
8 te,
3
0
U.W.
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OM ON OW FF WH =
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MICROBIAL E SOL
GENETICS rn
19 REF:
1 2 7 8 9 10
. Free Origine t 4 2™ day Vax.
poeiished )
8 Are dlAcacs
1-4 | early + + + — _
“ Vv nw t, ry V + ) TT) V V, — V , _
— —_ — ie ‘ +t “yen ~_ —_
2-34 blue v tor + allo remetre — alis vevy Spell
; (- pepe Petey ec C1 Rew y 7
9
enk Vv +, 7) ,¥ ; t clef Wea ty —
= blue] - end feu) t ?!! —} © 4 Nev
sew tra + ) _ pp" iat
3- 16 Carly Tr tr + _ ~
“ye t, 7, Vv +, Te Vv vo
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Wm _ + (alRev). | smal ai Cas aterm) and
= Aso |V 7 + yseu. [rev
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Vv" im this Tan is Ae fi any tel, U J prot psp! 'tatect
ow On MO awd —
ow ON MMW BW D =
U.W. a
MICROBIAL 5S of
GENETICS Sor |
19 REF: ee
1 2 3 4 5 6 7 8 9 10
woth a doors 4A 4a - a “4 |
ME S- S DW Clana UCL pion le Leb flee
af mL wy een oy b el pees :
3 frothone. Ow Fad +t Cha ) a, 6 e (fob | oS
4 é ie
5 “ Wrst tek ~
6
8 U 2 bea . (@) a) +, |V Adak ¢ Fat roe
9 d ax ee
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1 gre bute € N
2 + rn
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3 q. + J —) Vv bee | if ath Waters ly ) 1 + .
4
5
6
7
8
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9 “
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Dart, pen: lett. 7 4,7,“ ¢ “thee
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2 -
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4 f Ad '
cal
7
8
9
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1
2
3
4
5
6
7
8
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ULW.
MICROBIAL To “|
GENETICS
Dex * 19 SS REF:
1 2 3 4 5 6 7 8 9 10
go 81
Wl Freem | so -~r Fier. t Cla | »
2 r 1 3
3 A . Ar ‘ fone tow : (ge. . “+ ) bu Hee -F Vy
4 -~ \ = 4
5 B # gage Une " : ™ + ) J
6 f
7 a a 3
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° 6 |
f a ‘ * i co :
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4 ' 2 4 ye |
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6
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MICROBIAL
GENETICS
Dec. 7 19 se REF:
- 1 2 3 4 5 6 7 8 9 10
_ 4
241.
3
= ; | Y Dey - 4 Days ‘ Day } a Deys
8 SE Ava B bac
2.8 (Lag fpotate — Ginker ) = t weak Ke weak
3 9 Sr Vv , vG44,-, vo] v VIG, dack + lt any )
a ) . -'s s Int ire 2 WO pnet 4
g | S-S7R fron ~¢ egy yt) | -| °
a 1 Ota flee t Tron ) + - -
3 2 Vv 7 Vv v (#| -and Nghrthish v)
3 ne pot +
Cc fal Se S74 Fn ad = - - (segregating 2)
7s Lac poe - - t +r |
3 6 Vv v v vV(z:ve-h
_ Zz - co rd put ?
Db ‘ah S-S7e fron - ~ - _
2 a mk plate v V (donk ¢ af v right CV (“pal U5 me poet:
3 OT} Vv Vv (vo e +) iY } Vv ae es "
ez | S87 dake capital - - 7 =
2 2 _Leeom Ore. aad + +
33 “and ligt Srna! V V Y Che prre. t+, —‘s
_ 4 a (ve light SCE rts atlog
F og £-5 1 dark pagilleted) - - Td - - oo )
? sll Sera bee. V v Vv V (we gure -' 2\
3 7 4 Vv Vv V V yio er< te
8 v v (7's seqven qiting V wots
. 9 .
© 1 ofS STdmkh yf Smad - - - _
2 Crt pits |v V (Lett dak iv | v2 | segregating @ + types ?
3 _ 7 —ad® jight Y) - VO- = igt v) t
w2 Vv. V Cig & dar v) v No~ 54) tar 2
. a
H._) alge 57 dake _ —_ (rererte | — +
25 Ser | Lac a v V co ‘ v
2.6 ” + ouely vastt lad readi—y ) 4 - Seqveqat xy
& 7 V Vo, poe t va ty es v>
8 .
9
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. ————] _ ‘ we teal .
So Dal / (cla vdet pit cert)
a _ 2H. KH 4 Dota eck “p ay t dart Seutes Lok) streant ed bac t Ara
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6
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GENETICS Fo.
Dec. 1219 5° REF:
ys 1 2 3 4 5 6 7 8 9 10
woke
A ao GH amd | Bae / 4 oe | B- Che e4tedld Ae pretlne
5 Zam ad AS. Aig hte, (wheker ) er eg 5 ohh, aot af tf. y
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MICROBIAL s“o Tr
GENETICS |
Dec. i219 £8 REF: _
1 2 3 4 5 6 7 8 9 10
al Ara bn hae). typing of siugle colnies ftom sb ED. fll prekef
_. A voce fe
. 3 Fram |. Ora .
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MICROBIAL 5° K | |
GENETICS nes
19 REF:
1 2 8 9 10
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3 en. % hac
4
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MICROBIAL Po Z
GENETICS — 7
; Dec. 12 19 58 _ REF: _
{ 2 3 4 5 6 7 8 9 10
_ 1] Testing. ef bee test! system.
__ 42 .
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6 M bac. Meth - | M Lae (Bi
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MICROBIAL ESO M
GENETICS Lo
. £2ff2_19 FP a
1 2 3 4 6 7 9 10
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