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Hf of Ent x Jose at | 41... - . . | cig # JoS2 T, . | 88: 3936 |( Paras) | 3448 Gere Az*)_ 9 - . ol OfAC ) Spon, ry ap. im marta ) 4 rw eo rz ” Cone P . 3 sl : ad wetr, . ; o Meo aud 8 3 ULW. MICROBIAL GENETICS Le 19 1 2 3 4 5 Twn =O 5. - 6 is . 4 wu 4A sh 105 106 ug Nf 1B | Oe er: ME ZA 9, 88 3¢ S2 ; 28 3A 8) 6 4 38 | 4A ; US 3*O | wre | Nig ew) Wo rd ro re ee oe Gang, & Aol 358 []v ual N3C00! wgove| vip F) w te! ca FB, Mio lpr sowod Ngsro CAs) 658) 27 (Ft 16S | 798 |WIZ00 nG.t0 | eg 68 W399 Wi2o0. Na 8 , i 9 * A) deD fret oh gered preperteeer 0 + (A-lvo 3 Edn om ch pradaactnee ay fb _ wat LA Ve", | nm oF ny 2 3 4 5 6 7 : 8 t 3 ° ' | : 2 | : 3 : : a ! : S. 6 | 7 8 9 | ° | ee i 7 MICROBIAL GENETICS Lo _ 19 REF: _ ot /S-. 1 2 3 5 8 9 10 - fo ete : il 3s! | go | Emmtert © 2 3 : 4 ta s5 oe oN & 0,0 ng 6 fon ve cf 8: peeree bar (er) _ TA 9 da og - 8 |. a . Ab Lv6e0! “ooo! VEU | 7 9A 2) 79) 23 49 Q _. 3 - a JB, 4 - - ly 749 - 5 - & A 6 oo “— Oa oO _ . 7 . _ . ; — 4@,! VN wed | Yun | Nv wd — Tho 6 | ow | ww O° ! TB | wae | ise Vv Lore + 2 —_ 2 . . 4 t 6B sn wee Awe! pleve ~ 6 7 a _ Bu 3 Q ! 2 3 : 4 5 1 6 ; 7 8 | 9 0 i ' | 2 3 | s | : 7 | 8 | 9 | | 0 ' | 10 ST L ie f a f fe- zo %. 1 | bef Wet REF: {4ot/T of g 2 2 7q ‘ a { { 2 pe % xis _ ~ gt, s— a ee i oft: te —_ Ss ey 3 - — Yop os fe 3 a. ¢— © By { bx + 3s 4o ot Rper' ~n ‘e ety of B, 19 s L, ° ee oO t~ an . . " : +2 4 es : | se .. ° s Qi. “ . 3 je f 1 oF ala ~ . t te t & 2. — Ww % o 2 ~ o 2 ~ " 7 Ww g U.W. MICROBIAL GENETICS Tso se or ah A Hae seer eae -a mo trnNOoOnR ODO -v1 o7tn OR OOO -aA 7 THOR OA O POO DS peggy Pea LeT+ Bs loo {o Go So. 4o Zo {oO Saturday, June 28, 1958 ,01/6 TIMED MATING, Hfr, and Hfr 1 2 ORC Cultures of W 3936 (Hfr,), W 3948, (Hfr,) W 3052. Spun, resuspended in equal amount of Penassay, and mating mixtures prepared in flasks with 1,5 ml ¢ and 6 ml ? and 2 ml fresh broth, Samplings: 0.2 ml at every time, dilution in 9.9 ml chilled water, .0h spread on plated (A) and the same for dilution (B) of 1/10 for Hfr, and 1/50 for Hfr 9° Media: 1, D0 + Sm Bh MM LT Qe Sm By M T for Hfry 3. Sn BR M he Sm M 6. B Lac Sm . 5. D-0 + Sm By L Prol for Hfr, 6. + Sm BY Prol Te + Sm B _ DATE We (458 REF. 14 © ia — 1 2 3 A Ss 6 7 - 8 : 9 10 OO “| ee Time mary, We 7 fe po _ LOR Ww 3 ¢ a 3448 Ze ! RK Cares of 79 (“4 ) _w i, Ctr) We 52. [| a4 ‘ , . | am yres prepared oo fens | pot 6 ok t+ El Gg Hit fehl - | | , t0| broth fap On ““t ot wey mo AL Ln we BD ee CEL Lote, 04 Spread ow iis CA) oil lie fr Hf oot so te “t: : Mol oT Medcnr : . DO + Sum B, “frett Lenn Yerec col lo Py 2. Sm 8, Matt, Tore. for 20) 3. fe B, Mart inf Te 4 +. Sm Meth, Ko RV G8, oO. (3B) bee fan ! 5 Dot smb, dee pre fe Hg, 30) 7. + &, B, : 40 . | | sol po | ULW. MICROBIAL GENETICS ow ON DMN BwWN = July (Ar of . t 1 2 10 TFSI ME- age st Aira Zz 2 3 84 4 ° 67 6 7 SZ. 8 9 60 0 6G 1 2 96 3 4 | 40 5 : 5 5 8 ( Medina aA wm|b, MT Ye, 9 ° Ara TT Ae MBI M8. T = 1 RO | wz, 5,0) 42,5, o 4, 3, #°) as, 23,9) 47, ¢,0 4] 2 3, RA | #3,170)3 6, S51, SI, 8, || 18 42,0) 60, 0,0 60 4 : 5 At 17,4554 )6,0, 7% | 9% 4) 34,63,0, 12e,%,0 Joo 6 i 7 ob 142, 15, 0; 13,2, 92 104, 3, © | Hy 55, 0 107 0, © )o7 8 9] 2S |80, 15 5) 4, %, 0). 99, 4,0 SP 4a,0) jo0,0,0) 00 0 t ( Mediu. 3 sn BM Yr, 2 —_ \ 3 Ky | Mid By A MB\ 2. 4 5 o o,3, 9} ©,3,0 (3,0, &©/1,a,° 3, 0,0 3 6 {eo ©°,4,°0° Oo, 4, 914, 9,0,0, 7, O| j, 0, 9 I 7, 0,7, 01 6, 97,4) %2,0/3 6,6 9,00; Y 8 js o,74, 2 Oo, 7,0 4,0,P |e, 4, 0; 4, o,0 ! 9 1s OC, a O oe, 1,9 fy P,0 1 &, 7, © 7, @,e ! Qo ao °, & © e,%,° | 8 °, Oe, 8, 0, g,0,0 8 am P) 13,0; 913,09 | 4,2, PO) G83, © (3, ¢,0 13 AU 1, 42, 01 0,43,0 | 41,2, 0} 2,4; © | 43,6, 2 Ya ab 0,190, 9 | o sor, 0 | 100,0,0 (1,99, 2 | jee, e,0 100 af |, 94,0 6, %, 0 (%,0,0 |%,94°!) 9,00) 99 30 % 100, 0 | 7,190, 0 |1ov, 4,9 |, 600, 0 | for, % © lye 35 |¢, 7°, 6 | ©,70,0] F,0,6 |°,70,0) 79, % 0 10 MICROBIAL eoul WS L446 SeNeTICS Fach Column: to -, ae Qauly #2 9S _ REF: ee t 1 2 co NS 6 7 8 9 10 ' Medi wan a) mH M | | fie f a _ i 1 Aira Gal Lac T, fi z. M —@ey &= 2 | 3 20 2 e, ° ©, 2, e /, i, 0 o, 2, o 4, °, 0 4, o; o 4, 4, Cc ! c= 4 s| 20 Ajo,o 1%, 2,0 |2,0,5 192,00 |/,°, 7 A,o,0 r,o0 2 6 | 7, 3s $$559,9|0, 187 0)13,2,0 (FITC l|IFC co, Meo |%b 0, IS 8 ° SO} 23,2, 0) gs aso 9], seo 25,0, 0 |tieme® |beeSpo | aes AS 0,0 A2S0,0.17,F8,0 2 0 44S 47, 0, 0)0,47, 0 |42,6 7 |0, ¥7,9147,0,0 |4¥7,c,0 3BL/40, 47 1 i 2 5 0 joo, 0,0 0,/00,9 43, 6,/ 3, 97) OF 400,0, °o 100, oo 36, b/,3 1/00 3 ' al Go 109, 7,9 |0,,90,0 | 87, 12.1 | 1, Fi, O 120, ©, 0 |soe,0, 0 My Fb, SF | /00 5 ; : { ‘ ! 6 | 8 9 Ga\ fra Tr fiz LB, Fai Be £ PO 2 0 | | oO O,1,0 ©7,9/51,9,2}°,),01;014,°9 | Oy,0 > Lo,o, ' 1 | : : 2 + 2,5, 0 3,4,017%°, & |3,4,0 |2,55 0 3,4,0'7,% 6 , 7 3 al 0, /6,0|97, Zo lk, 6,0 |b6,70,0 412,09 49,0 4 0,0. Ib 5 i 6] 7 C,40,01a,€, 0 /e,c,0 |2,8,0 af, 0 12,€,0 fo,o,0, 16 7 8 &§ Q, &, ° /, Ss, ° 6, o,0 f a, o o, é, Oo 4, $, go é, 7, Y 6 3 0 7 O,3,0 3» o,°0 3, o, 0 | 30, o : 3, °o,o 3,909,064 3,0, © 3 od 6, €2,0)7, 73; © |8,0,0 | 3,75,9:.2,80,0 | 4,78,0 | %,0,0, BL 3 2 3 a °, 7,0 52,9 7,0, 0 4, 3, 9 O,7,09 5,2, 0 7,°, 0 q 4 i 0, 79,0 | j4, 84,1, 97,0,0 | 5 %4,0| 0,99,0 , 1442,0/1 99,0, 0 q9 6 : | 1 43 0, 17,0 | 26;72,0:48,0,0 | 15,739 % 98,0 | 35,63,0| Woe 49 8 9 it 0,207,090 i3, 7, Oo | 0, eo, °? 8, Ia, oO é, I4,0 “ly 7, 0 (ht G ZO Q oo OWN ON wm Aw = U.W. LOUNTS ee MICROBIAL, Count: Sef 6 GENETICS ne Aamo Bach Oo fun t, ~, + uly t2i9 SY REF: ¥ = 1 2 3 a 5 6 Db 7 8 9 10 CN : kD wm £, / th nee ee oe 1 Gal Lac ™ Ac i B, Bi RA 2. 2 3 oO o, 1,9 ©, 1,°) 4,0, 0 4,090,090 C,41,09 | 1,7, °e / i 4 t 5) 5 22, / | O91 | Z0,2 |4,/,° 43,0 , 5, 6,60) 3 6 7 6 o,/, 0 0,/,0|0,0,/ |4,e, 9 |1,0,0 4, 2,0 / 8 : 9| 7 0,40 | 3 50/3,2;3 (6,2;015 30 F000] 8 0 4 O,2,0}0,9,° |j,0, / 2,9,0° @,2,0 4, 0,0 2 1 2 7 ©, 70,0 7, 1,9 |9,2, 4) 1e,0¢,0 |9,4 09 | 10,002 /0 3 4 1/0 o,8, 0 O, 7) 7,4, 9 3, +O 4,7, 0 £, 0,0 g 5 6 ft 0,2,9° |a2,0,9 , ©,0,aAla, 0,0 | 24,0, | 2,0,/0 Zz 7 Bs of O,67,2) ;, 66,0 56, 6,4/37,27,1;2,65,0 | 670,0 67 9 oj] ory 0,30,9 |My 0 | 4b, F127, 3,0 | MIF © | 30,0, 0 30 Wl 7G 6,940 |1,9£,0 | 6F,al, 10/72, a7,0 | 6,93,0 |F% 0,0 99 2 3] 4% 0,6, © O,6j0 | 554,001,322, / |0,6,0 6,0, 0 b 4 | 5 B | 6 Phimmwanac, ~7) mm / hy 7 8 Ga \ bac TT, | Ae &@ 9 i 0 oO 4,9, @ 1,0, 0; f,9,0, ',29,0 ! ; i/0 4, °%,° 14,0,;0 0, 0,€, 4,0, 2 | | | 42 0,4,9 | 6,409 | 9.0,1 ) yo,2e@, | | 2 su o,5,0 S.9,0 Ss, oO: 570,07) Ss i al) 7 6 ©, 30,0, €&,&2a,0)18, 2,2¢) 30,0,0 30 4 5, 12 ©,2f,0) 72,0! 51,22 aF,00, 8 6 7, 2o ©),7),0 |23;57,1 (3, 47,27| 81, 0, 0 Bf 8 g| Aa O,101, o |r, $9, 1 |Z SH, 4O| 121, 0,06 lol Q ; ad 0,N,0 |5,6,0 10,7; 4 112, 4,0 i : 1 2] 26 °,f02,0 |5743,0| 414,26 | 190, 0,0 loo | 3 | al| 30 0)F),0 | 4, 4,0 0,4, | &,0,0 8 | s | : | 7 8 9 0 1 (%o2 REF: OPT(M, Pucse | 10 IBIS |; Brot. etn, Joo, | Joly tw wat borg folareTid gente 2%, jnotileTid into wormrd Pere 50; | 4 1060 —» bead | ss NS fio \ he | ' So! be! -os B lag 33. of Bla. sing © o.} 0.02 OL + Games Ib 19 Mowd, 26,1957 _ ATE: Jun opty 402 - Pee Gokt/tet. 2 lace/jot + T fist, | 7 - 8B 2 9 10 Time Conan w) of 4/31) 12.9 24/31 21/31 68 ' (9/31) - 29.0 2") 6/57. 8.8 arfsz | 39/57 64.¢ C28/F7). . , 49,e 12/94 1.7 gev/s¢. 68/8t a C42; (3 | Sto 101 43/79, 16.4 33/74 , 61/74 77.2 10) (36/7 oo ty’ u/es §6.9 | S/eS 46/65 723 fa oyi +7. ; 60 5/50 lo,o | 45/Se ny 37/50 Feo (18 / Sa) 3C.0 o' is s/7o | 7.e | 18/69 48/70, bE tear £693 Uh? oY, 6/er LS iq /seu. 3é/sve £4, Ae / | ey lO" ise “| Condapions (S'~to Party. Ss an MOE rowtien C FO fe No. f reesei. : DA 4 fie at Leng) offi« prtge if pth 30) Baranye wf bead ben ep rarttfett poor dak & Cab tq cawneh oT wh A ak | Brite - O, | unt ey My andy Ld LF ry om jingle di Calrnn wer LK pe (Ya rity Ane fo por pleeg Ep pabT (IY 95, met percrrfal teem 40 tf mpd prs. 4 + fhe (~~ Ht , wrath rycaTeak DATE: March 24 eo REF: PULSE 1 2 3 | a | 5 > | 8 | 6 | go —_ woh . i a | Time 0” | Peat Counrls _ Aa on Y O° _ ae... 3 Amount | ptedted Time 0 2! s! to! ( Co! hoo Ko Is 7, ©. 11 zat 47 85 77 Weg 136 * upt 14 167 136% o ~ 2 27 io is ty as at gt 4 23) 3\% + $+ foe ales srepealid | | 2% (Pulse) °F 2% Mo, Rec. i. wa 0.) we % ro | 20 oor wi 4 y %, Rec, oO i 2 baced Co! on He nut, oe a 30 2eor 2.0 fF ~<_ . y £7 7 4 ° Dap Nore net * ~ in mA, iw f E y : t An o F => bad dal toot, [oo - #. toh | ? ve yo oO ~ ’ : ; 4 92.8 1 4 ' “oi Y “/ foo §=—l ho ’s nN a Ag ‘i £0 7 i oe | Micheosc. \CouwTS OF | PALE MrAL Gros i | | | i ¢ i. | E300 : 2B30xI0 [ek | ! | | 50. ¥ 2064 1Y x to hwt, | | | | | | —NIPUTS pe wet 3060 S10 ft Roe He JE- = 1 Go Zoey Joo. foe : | pate: 22/4/58 | _ a 8 9 7 10 a 1 2 3 ! 4 5 pe Tren. 3069, 3064) cuttin, we Iho a Wee eu Mut 06> iyetake , ComctwotreT eck. fox, T we wee bose | o 10 % Exp |: B.2wt +92 F-<= tr) 30) me : ttn rep. repectid 3 Exp lox 4 ao » u | o ot by ay 4 :33/; aay, aa A ye Exp. 3x & © x rep eedd | dy @ Lat’ € asec . Aen Isloaoo ALPws A: ben |. 02 wkd fo x% : Ne | wa “uJCG, a 3x : /o.3 om (ot al fasipeyiony Foie ejCn ayn d b it 0.057) Ahead a a (wi phelg used . PO tort |; mod, ob Come AX A umnrntighte 3x / lox » bo {0 REF: 19 U.w. MICROBIAL GENETICS ay ae Mot te be —- NM tT MH OR OM O “AM TM OR BHO -AN mM FTN WOR OBO to) -— NOTH ORF DO HW O -~-nxN Oo FHM OR OMA REF: 19 U.W. MICROBIAL GENETICS -A mr ¢t¢noRrono —-nm ¢noRoOnO -vV OetynwoR ODO -nntwnuonr ona -nxn OFM woh OHO U.W. MICROBIAL GENETICS 10 hey tL rican Pt wat Le pe. lA Me Ay REF: 19 Orn wor On O ~-~- NX mM TN DOR OBA SD -~ NOM FY YVR DHO ~-nXNmO rTM OR OHO - AMO FTW OR OHO U.W. MICROBIAL GENETICS | . REF: ‘do 2-& 1 6 7 8 9 | 10 We Lic ae . J 4 yee - t c - 4 oO / . a | Te 3960, wezolhl Cy az ye Any. ao 6H a Spe 2; oO. gud for Ke Lee ll td ot ee _ 2 . _ 3 a an 4 Med. my o SF AL tea oy Karr De ct S ; _ - asf. 4 a. tak ( ‘ A, Ctl nee trp fir, Vs-ove a} om | | a _ gd a} - . _ oO oe ri we E ro qve Ail | _ 1 all. . | ALL i _ 5 . | | tft . BY { . _ 9g Pa. A AC fiw ove 2. ah 0 r : + sek | i Bg Ak fisv vw © 2 Coke | ——S ojo 3 a DH ad five Soest Si 6 E ———F ~ os 7 8 r AéA and 9 _ ol. oO ON AM Pw DW & %)- UW. MICROBIAL | GENETICS Lo 19 REF: ( 45 a / . 1 2 3 4 5 6 7 8 | > 10 fe he [aye ve ©] eb AS Y bei lo €e0') i Oo jp At bo") heya LY Mets Pee bo! eo? . 2 BGA, | 7b | hfeo mB! | I foF (OO 77 bo! ow a A | & Cc D FE | co AI, . _ — pole sl aol | 4. O 2 4 2 Oo, Do’ Ao FH. ee on . ~ ‘. i ' 2K, rit. a: ae" 5 18 pert syyecal 1g GO | Per AeA 9 cn. . . of 2 ali | loX 2 1] Pemeteg | | log 2 2 3 F- S H Ll J all. a. . 5 Ww 8 g Gg 7 2. _ 6 . , a PK 1 ad | ss | gg sl 1 BY - : - 1 | 9 . a) | a call. | all. -f _ gag get . 4 . if of : ral . BOA 2 Voge move hae Lag cal, 4 | _ 74 mAArl£..f/O is. 4. Caged icy he . . ' Bi. . 7 a : : : 9 0 oo ON OM bB wD = ov @N OW Pw Dr nad tr if 1D tH aL le. Ler LC wood’ wa) eel 41 A. The. A hin 4 ese msenste T pater t S ULW. an MICROBIAL | GENETICS Go) 7 (Moy 26" IVS. ner, (QO 2U/S Ep fe a B + < : ~~ > ft i ! 2 op | Ere a a tery | 20") tannyhs dele, Hrd, plates (eer Nol A b pry po hw = D) Poa ~ ‘ x x ov _ : b > ? CO PND HW Rw De! : 2 254 g oo PUD MM a wp UW. MICROBIAL GENETICS Lo 4 re ; : is Mey ZY =, 1956 | REF /yo2J®& 1 2 3 4 5 | 6 7 8 9 10 1 | rons lon ple te) 6A ‘ OF 2 — 5 = 6 ) OK LON ete (data ho ieorfa eneh 1es@¢fs— wn fe 7 8 ptlow wp Ay fren cy 9 L. - 0 ,25| 3K upe foqo) t+), to go xe GR Belk (o.n/ 1 ra fern. - then aha te tats Puray . Uy, ot AeHQ 3 f (pF C tro ) | 3 JO -# le BGA (FB) : oO. t10) Peo (PP). : 6 | | 7 fam d of Lo’) ¢of C,OF oh wr fo 8 fe cf / gi Fo _ | F 0 >) . Cone | rey a AtYol lo 7 1p — 3 | 2IrrT HO ese = | 1 caeck wh wd un epord oat wy | SS a - 2 . rlasgl a aa | 3 fone oA le, 4 / oF mn bw fr & : Xo | 4a’ | 7 a PB 4 uy 2 9 0 PP 8 14 1 DP / l/ 2 3 D 3 © z3 4 5 6 ? 8 9 ° , 2 ! 3 | 4 6 y | 8 9 ! ° | : DATE: Mork ue __REF Efhq of mampul i ~~ | 1 ? 3 4 5 6 7 8 9 190 14, US] ( ph +lo we of bnih —» LoteaGr. of obo, 30by_, Cte drm fot fife wtry ile lols, (1.34 Time oO : OF wn Jove +/F me 2064, +5 md brit wy koTAT,, 1S 40 10! Re ee foo wit {wt pp —? Ane ito povreel S68, efor | 10 \ ae Koo —? ° WoltAatt pet Pe(6) \ " —» Se’ Retete > a CH) \ tt : fs im obi Ceol tt, > 50! Retatler a on @) \ “ 1 60) viaTis —> ” — C) \ Ps t n Folvi@RATOR 4 +p te CD 50 wilh Od wt ppM 92 50! Letoter —p CA) Akgo . fom j oA td q Se de) me fob Ant pera Dif+ &, : pry . we D (St b) | a VIG Sp 4 Pavey ase at Lo oO : At}. Ye £lDn. in Jornakiiy WK pr Arn orof oR Lo net | 6 3060 Sto xlo Ane | gocy 470 x10 6 Jk ") Regeth A, ‘° coli Com bipion B ) .o) | hau pelected pe d / oO ! 3°60 % Fr Polen fi on dD 9° (To be repeoted 50 E 9% r of “/ man St 6B, m (2!) aan Fol: urwn DATE: 1 t/s8. Ge rer $403-T 1 3 | a | i “| 6 7 8 9 / 10, Shacy 30/0 406s - Jap resetectic he he. Now | rept ow afer ‘shoke. Bvt, trated OVernns gt 2 4 me * 9 wt ad rotted. ti have, os net Jofo, t+ owe 2064 + Swe waewed brurd 7 roti | ok : +3066 : Ye, 2 5 10, | ose ($e he ° pel. of 0° fine. : ! (30¢¥ z Ire —., os | Aft. 10! retEen: | | | A) KaoXé wit {me pipclle : [ore to’ pune : 0.1, 0.02 @ a m™~ beth waren —9 $2" rotaber thn play aebon @ ©) fee wile Ont nite py OD ed) fam os A sing chilled brat for 40 E em, thas rahZer on _ Cue) _D) Has de th {mt pier, beemcing ~ rhe 50!» play E) Via ace wth 1ue pir, Mm Wormed, both + 19 rlmt 11h poraiged 7 veteitar Fe! —_> platy, | Pests ~ ns Sib, Coun Lt pats tmeys Hee wee, Ai | An B, ! Be Cy CL | D E o , DD otk. | | | ! | | ol ple: (2237 ZB w800 197, Ake 238 436 | 2 at 8 @ inschodey "Wieaes 30° 92° Soa, sect 3/e 22/40 j3/38 12/26 \g 3/29 76a 2/4 16 [tt 73%, | S58, x 4/50 26/50" fo 7 ~ 3g! Gols/n er 5/40 0/ 85 It/80 18/24 z/iry o/s Me 2.4 f 257 o% 137 | Fe) OT 7 [n+ 1o/ht 26/41 34/86 so ne (2/2¥ Pyne 16/4 55/1 5° 249 ORAL BAT 7B. | (68.28 | CFI ogy | oe ed jyekhe 8 tak bel {cy at both. Bea be 7 Steyr pat i | &e fg’ Co! he! Go’ Flor to Pisa by | pe DATE: PLATIW 6 4 [-Lo00tvG_ 20: 30 40) 50) /4.38 3 4 5 REF: Ito 3-3 7 8 9 wl fo wwlh {Ss Pe Quy \ brott 13 ORD i. —_> ALL Mm [ow 4 { —@ ro he — os. time Y t Abe 0 wo tame aio oS Con. / jn ft flretek pole #. se6o ee pometne bir immed, ve pohs bats. po Sy | Pte | A OF. fo 9 Fae + tor raqiCe ALD : | | te LS ik foo by ) ait a VY flere peat } foorens gt mh 0.2 bt ptt Hes JO6¢. ee ood f mn. vat w49 Gabe mit 10 AL cu ue Lwin AK, bache S-to deed teh ver, [¥O3-3 © pens 3 4 5 6 7 8 9 pgs Se - , PD -—_, ? , ’ S fg |Iiwt Ho OF Kye Conk fee 8 on (ter eens 5 299 4 oO 8s: “7e oe ie) 397 (52. 0° N 400 (82, 600 42 coo noo 162 Rid Crd) cgend pak Prat wen picked. Jummory ef S rykons Spread need : 0f27 3/50 0/27 |. 14/5 9/27 | -27/s° o/so 3/s9 (3/50 14/50 36/50 31/50 4]so 10 ?/ $0 1¢/s< 2¢/ So 26/ Se 3 $/Se | | | | ! DATE: 7 REF: Igo 3/4. STABILITY oF PAIAS TO - PLATING, To Heck “f (2323 fey 274 an | ne fete He, Soro | xe 2054 to iin 5 patug, SterTed gnc. of 2343, U7BS 3060 F064 | Unned cy et Abt 20% Poked LTA Y op and (0.05 plated om warn fe A 3066 x Foley Cols : ; eee B 306 X 1730 0 OD C 2323 XK 3064 oO Q D 2343 x )3S 6 e Net Picha fr lac [yrgrOrs| - At vere bot Wy pve T | U.W. oo ON A MH Pw DN = meron Lv | oo D Moxy 19.50 rer:

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(oy h wb -(YY « W308) Ar sft Heb” MEY fg (2 of Here el 5 S Bs Ayy979 % 308% al fy joyr/s1 A - VY, » / FF / D KS, I 3 YtAT 8. hf pet NRe Fgtetur G UTI@ prazeny | uw Pro yy PO oo venocta p ER 148 4 ou 19 sf . REF: oO MN DO HO B® Win =| 4 Mada. 10° °P 44. oM ON MDW B® WD BwWN = oo o~ Be W1974, WIPIS K W3081 are bese pr tine mr Bae (nocmn!) MAdeving ? iv t | pa val ire | hug yon AR O prot mart ? alt — at OCAD> : dae ovqgoraun on oO hw WN Oo W8 ON OWN BD = 3 64 T/ow 3 F SEG lt, MM | { S¢) ati t w — | . i : pB) | ft Oo een. wee ee eee en tt ~ a + . Sey ~ ft fe +4> @&6 (. > ; Yn t + +f .. : » { ® Hoty . {s $+ 4° ! s . ¥ va ® }s ++ e } +* He ++H + ++ 4 = i Re ic> <- NIN elem -— > Gyn ke AY AWN Oey 4 fa wy wn . ° ee e ! ¢ Deen pe a: te Oh ee ney ! . | t 3 Oe TYLA eR ND Oo eh . } ; o . LG} eon ¢) TYR wa te AD PL LAN ot Ae PI Pry uyiny + eA te OD 4 | ae oP ee eh ey et et Et do is 3a5 | : : eM . x z3 $s Fa | . se oye | a {|s : i : : ALY me SU +44 4 | | | S He tditemy iy sé : oie. sj saa kay .o y Her > t i“ trtes (Se s [444 +eF 4 4 tl u Finan an ahs Wr on oe MA ce ~ LWau . . Lovo neg” ~ 3 3 AM. wa OY TAR A OF MELA LA en Von myn Via OF Rann PA Ay a nly o 3 + 4 wt Yue trey pp ee tte gs oh tree Ct lthtipg tree yey te terecry ee pgtt yt oo “ 7 : ess _ ot 2 ccc + Wie ttt ole Hed tO 8 FL 4h MF tt ests ofr tees [FH tty. PEt eH fo ae Ye OE HTN LE | va , SWAIN Ne Hen [D> eu 399 \ _— . - “» ean ay vy fun ca 5 HO fl Se Nady ye OP” pst A oe Pet +o terre yp Ate atest a it ota eS = = SL) ~~ m i i a * - SW : : we wi ¥ ’ re e) +) i : kg Oo! : Oo oF 7 5 7, - oM: ”. t 6 10 wu Le Ss) og KAN. SRES _ > N ys | Si nN Ss Ay Nopeg ore \ Oe » oD ) IX Lo — Ss. ion ~S a , A ~R be, p = ~ 2 A a oO ~~ ~ ~ “3 th Nek OF we ro) Wes ST ey : SO Be fon 4 ©) Bos OG epg ge _ cee ee - i dy HK ow wary — a Ss os Fa . 2 . ee een weer et bese Tp ean leag set tonede Fett tev eee eats wt a KE Wows =9”R “ea je ynnut(Y vnraetu xy) Bae OR | CK nev) 2 YByxnYraann yyw Kr2nh Senenmnnny we Pa ia =<] c 26g 64 & Prete ghey ped gp tpattit¢t s speva ted srs ot LH + Lt Orr ees ee: ~ 3) H, (88 2 235— 3. 26/ lfc): Rerceudete te Thececng Conf all gli 3” 73 bali inl, or 3b md Ao Acts talornzs 7 O, Meta fron ff 0 a bY 4 37 39} ws 4 Pegredbice : este om EYES Lae. Jeet Le’ X . Yo avd Vg ard Mal. Ul 2 meat shod bee Mpb~ S*- Toth r bey cures bvebr§ fear Aewell bls agarcet W) 3) 32 (He c ipa) orf Ml midurer - Who a Heb ~ (Cath? ) G9 ThowTt T conrkiaf eels? \ , wk . /4p Uf. frke a So rues A, C, rb) # reehren K Fhe | REF: 10 ‘ ‘ po 19 UW. MICROBIAL GENETICS o eh ee o wt wo w wt Co) ar. In ~s -S _. _ 4. . Se RA LUI ne {a neg 7) Pile BU & Dy) -;» a NY-s nen a ny) AN NY } Nw : ee : ot & ce? OF 3-7 Bie —c ~ a 3 "= way Pe = S ~9 wow 8 “Norn on aao “NO TH wD ao as etaagey : | ULW. MICROBIAL GENETICS IPP Po QA Ey cHoORVKA HEWN DM CK GVH HW RUN = oCMVDNHH ea wWDH CoW AN DU ob ‘~3 Noes . t : , ’ v . \ . *H0o4 ZC. Teed ¢, DATE: srafse. REF: 10 o : ° | ! | i |b - ~ i 7 wy - ty her * “Acar? . : Sie MAD ELPAMNMNYA Gn ar 999 ox vy Ory os nflrn min Dna An ay hy A Din AAAI » _ 3 ate j Semen oe ae wane eae 6 aan op sae ans anager g amare mans earnees samaegann a - tere wn a ee ee ee ee - - ee we tafe ee ee wi C7 ata gyn annul neve rw Uy Manan yy nuctadtus MRM A DAM Ap) 9 \) © { nt 4 / 8 ae : : oe “ “vat = ES ii] | ‘0 QD) fh) — - oN ty wrt + yfraat HIS - ++ Gteeaegtt ty + ttt ttt tat tis og 0 oy 3 —t- LO —— . ~ WEL AN prrsunen a) 7 ; Wy 4 Dn Ly “Den AE OI AA NER CAN yy WAND RAND A IEL A rn 7A IAW IY ” : : Km a vy, Wd. ; 3 wy “Y elat ANZ nd Atl aeun Qe Nhe YOM yalaned ant 3 < Hon wifey) 3 § LL 32 x | nu ! a ! S++ tle thr e+¢ 3 a+ +> aly +d ' 4 FRAY tg 20 30 40 50 a etme. fe (Fr be, GENETICS 26 Mas IO REF: 1 2 3 4 5 6 7 8 9 10 ited v= =f 740 = } *Y, to BoF4 vow 3S. LL hac *S oUlgr ta At fe wae 4910'a0 felt oF” Phd pope pgenrtecs Oo f& WON _ 5 : “Hy 6/2. son 0 2027, MIT = th J, , Whar > Pay coon and / 8 Cortana F: ' : ~ - oplo ikaw fooler dike Phat Arid wiht aie an 0 OMe alc. Mae Kuceeo F PU rere tele : : 5 6 7 8 9 0 : 3 i 4 | s | 7 . 8 | 0 , sige b dbp Matha ib | LP Amarin ped . . a ; aA , ; ony fe dk Cates tanibe th ot , oven pated. | ; : | 7 a 9 Q _ 1 2 3 4 5 6 7 a (4oS- DATE: UY arch. 25, CALA rerPRoT opt. TIMING 1 2 3 4 5 6 7 8 9 10 9.30 | #3060 w+ St bk 2 whl. So | he ¢ Pw C, ned ‘re lo? A fpen. fn’ . 1G.te proG nr Tha hifrar uery, c7)oth. 10 A306 nH > potter for YS! | | City pm pd Witt & me, W Or ws tot. (Was 5064 tn Dy nrecliam 20 ee —FT runt B / [we ie | 6-10 nb 4 tt Ye, fed acon + Unt 4x0 Jee thane tefl | x ~~ RoOTATOR Or . T k . ,oF | PO, L ,oS px. eye 5 m™, a 5 Pe re . L he 4 oF pl Co So : Sey ae 4 fom, ° to wf Jomure Some 4o—, 6° —, bre." Pe Ate parm, of D(4,) ond. Blac) | sO Micresco counts : apfo Cpa: 6+ 108 ee. bur mrad : 3% or 4s; ¢ ¥ /. 3064 on byw. 410° / nb Pore PLAST oer REF: (qos t- mr Ainf B, ’ 5’) le' Ve’ 45's fe' | Me’ 0 | 0 | oe P Gad “04 “te : *° ee Ko Cross 2 2 3 30 89 He oe ae °o 1 oO i to tt 32 | | tol Blac Ag brdp 2 Zw =~ 233 eo nm ° ‘6 Yio Over / 4 0 3) 1¢ | Yieo ° © oO ° * o iB he Dramrp.. 9 4r | recent . 37 Lo . ? 7 ' at ? wto* | Coe : Oo . . (sewed 0 10 Ye. 40 60 ite ito Viable. prt afer ghechey : « Foofl gutyf 61o* . wie™ Reconbinadly fie Zpebaplt oft 60's 2.3 tol /rmt ot 4 Flo = 310% Not . | oft peaemeneky te OF cont |e inte 7 pooteptotty. | At tie Ve! s) pm pepe Ye. 13% (Hr por | Ree reed" Yo’ 10. YH rm 60! 1g. Fe [r0! CF “Io 7 Thy geo for tthe A Gal aby ooveg 30 Pk + é 4 40 50° : Ha | = ar °o uw 2) than, ove Si tos wow be prportm, ete: dh con @Ccau | ie , Ch prt alt, | Tres 14.05-IL / DATE: Me. 24 REF: PRoTert. 7 | 2 3 4 | 5 6 7 8 9 190 12. to 3060, Jobe wht, > | AIO wd | abt. 2 &y Net Sa toa as jer : a. f 13.20 luk + FY Lo 4 fo" ponce. “> pre 3060". {[5-To Rewdi pose , pty rj{prarnd pools pate nr LU nude ein 3 Atje 14.50 Jobo / 864, 3 ~, 1 | is.s9 (sXus/,, reYuguand wy oe Zoho Sedo Joe. So6 ~~, te Ke re — us taf 2 sal ae NE 4 anny ¥ , “ree as Poem. 1% ) oo _3F e a 7 CLF :| OS + G5 wk wale, j {ml 2 poured. comiahes ie kad 16.4% A, 8, DE Zo! witatr — OS 4 UT wt wake —7 nein St B, JL DP OS aT nt frwske)yt —y Ofer [ua Co’ de > Ay. “t / ve OT we. hy dan aI pop 2“1}6 0.05 uf foam (0% 40) added — Mi cro svope co Es 3060 420 x10 Jk 3064 |, Feo xlo!/ume 30Ge jnshe at 5 ad ele / wt ~ © reds. 3064 n 2.7 jo ork "4 8060 pw a: ayfk preted Anke f, ah wal, piphey “p ond aloet y mynd ~ DATE: 3/2 4/5? _ REF: h4o well TT L 30” o’ ( | Exp. A IS | 33 Sy ee | : B ° o o | ° ! ' ‘o D o o | fo | E | o ' / , Powrela Ofom potty fom c ¢ 30 fo) 278. t (3 o6G ) : 7 tt. 20) Cpuelugry : Joo Lu, etn te Ft, Te fare fringe ots (fete ba rey ofa Og yp. (403 Heng 30), 40 50: 3o6o : JobY : te hectepter Prepovetnn ¢ ‘ | 3 | 4 | 5 7 r 7 8 - “| 10 po - pesranntty oy 7 - Exp (Mor rT & Yep ead ed, but aftr | burg E nel Len Dw, | 9.057 am. / nt s060 oveeuighe otreh + Gud bre7t, -? Rot | . | 10.370 1 wt 3060 expen. + tat L, far Coowse prnnlnr greet. AG RTA, 10 Pon Hpk Ak fo : {¥, to 306% tol + tow Grote uy ! i ee ae woke Gott, 3060 @ fox ——rd.1 nh + 09 an On om 8 Lac, ~~ if te ‘ nate) 3064 jox ——Ta5 Nae ane owed > —_ 2 | | 3060 6 soy resus prmadssle im ew L,.: s tox | re gt pen te 3% (Op l. f Joo e) nideTed un Wwalie for ave 49" than: libuted 20% witt Ll. wradian wrewmed o& Oo’; tuot + Frye Dw —y 1t.0 0.06 avs 0,0 om ein lt By | : y | [ | elo wl bute, wollte both (+ 0/68) — ptite fo. 05 wo’ a ’ — + 10/50 jo 7 . | ug! 4 % Co’ 7 Vie DW —> -X- Co (irt- eoTT 0.07) 4 toe tri, —y ~ 69/0 (72. 0-997, 0.01) 1 2 [ 3 7 | “5 - 6 | 7 | 8 ‘9 - 10 Bo Coe of weds tlle ce poten mein | | )O! +.9.9DW, —yO0.) Ems bme plot. 2bb6 cok. 10) ( Farw. Jems oy Ah Gor & Le, ~y °.! E Mb lee 4 ewok, | L | _ | 7 teens we 6. a ~~ (dried up) 60! n > * FJ cok. 20 r | a tint Mo, of cath, which were proGyptastid 4 nn lene wie. bur 9 g te okt 2-5 to Amd. Hernce f-Zn. fore % of te ordie 107%, ! | : | a) leo te 3D 4 >> Ge Lat ites | 30) X- 0 K-19 XX" XO KH & Feo 0.08 0 191 oo eo os | 0 sof 0 41 362, Goo Mf90 = 760 | 0[ be 10] 92 10 | Uo veise WF bose 8.051 44 178 De ) wo | co ; o at yy 4 iz ¥ \Jo0 = 609 wha oF} ay NJoo | ¢ Hote te KL | | the Eve Knew oe Aaa zo/4e 30/30 ysis 60) o/so e/ So [Fo i/s0 | fs §/se 60/0 [2/50 oo! K-10 X- Vo X¥+30 | aC x-bo. gts — 0/40 o/s of 50 ute o/ka but | o/9o /so Use | q{se (0/50 qT 4/40 27/5 14/$0 24/50 19 /5Q 30) 2 4 Poe 7 ft trom pratetens | 19.30 am, 4 wat S80 3964 ~ fo tel, La, i 4a. Fe pes poorly few, ohne | sob¥ nell prdwe ; Z- Seo +10 C/o. Pare to* Wah added, 12.35 nbn of pote, polt rietad C4 Gack bolt {S00 5060, 3o¢s alr, creed : gefo, 2064 protien s Cavte: ey mL 3064 | 3064 2 to wt J2607 Sut "1823S ee 7 . : (Jobe Se. | $e: A C D | E (30f0 | 0.5 ose ~ 2o¢0 4 aa - Of OS | Jott g ~ OF os 30) o¢yY inl oT — OS | ~ wel ° Ate i | | ! | we 0.05 Ble Vie beet ¢C: 3060 o Ol mw + 0.9 wk e804. Beg Vest | Ee 3°S4 6 " a ‘ 16.0. foe virakey Aw A, &, yn, €: _ Vie pw nae OR + OF wt wee TOS mts, '¢ A bre ow or jrorv/e : A, B, E | 0.085 fem bet Eb gr Ufiw ati te 2 @.05- Vive aew ly »D : o, os form wT ate, | | | ‘. 30°: 40 50 yarn 3% 9 foe an ° rd bP ay 7 ee 6 3 SK’ \% -t- 9 ° et a t Oo | care: 4/14/58 ee NYO Y : Surrurery of fprpahiry $ sm & Sere _h, | F "Gels —— Git) Tr “gqet+) twee TS —~ - A. oso g/so | vy/50 U)S0, V2/ fo" 1 - 8) 8 Soe 10/50 | 27/5 abe - . eg 2 DO ; - v4 /o? rt/rd oo - EE 2/\oe [3/loe S8foo 0 /S% | 4/50 16/50 ! ~ Absa : a od nei ied Frm D : Streekg m EME lac. ‘ 2» bali, : 3060 ghorrad srtee; Hy pr fo, rhs . | pectin J 50 | | | 1 | PRoTOFLAST CRoffEl . | Ryton of iWos-4, 3060 30k4 BS og ial Upeuet 1409- ¢, tur aot Se wit Z,. J06O Jody rods c., 2x Crmeertahid fran ‘0 0 veri hy potaTad eekly, | 3 | Platt om ie Suc G6, St B, &St M s S’ A For 0,02 4 O.er 4 008. 7, 09k N 6 emt Avo 0.02 | Joo 207 Hon 2% i. oor oO Howe |S Yo Pes ! Y 00s Yo Yves so ?P New. Nenu. Ye 0.02 ! — Hoo 002 % 0.02 Moxey, ro fg ST op ln ty vad Onno Git [2 20, Afr 60" auorbtr a wate te Ar, (HK i) ond pot a CUM Yh om tor | Ae, oh pfo: Fo wt 40 06g (fh fry qT rr, { oy weds y i | Jesy gi. 2060 a Vere ww toaepat ion ] _REF: [¢o v- - DATE: _ . | 1 2 3 | 4 5 6 . 7 8 9 Se Seeise B. sue Ole win G, wie Se 8, a m» 0.02 s/h tern | oot: wv £09 O.0o. many 9.0%: weeny |, ae au hence >| AJ2 | ona 158 wt. + Amn peather 4 to eaters. a g wn (@) 30/2 374 25 31% Now 0 (0) lof ? {O JIG 9° fv Soe | Now Note (P) wv Jo0l0 nN Jouo & tooo, Covbty: 30002 — rant yma Gar ! lv 2eee), | 306 2 y~* wit. ye gk se Ope a eteely Picks | ic Ramey ; we . | | | — = . 3 [ 4 i 5 - 6 vo 7 - ‘a 7 o a ; oe fi + A _. a of Pp VOR-3 fe det Lae heme can til ed en iment ph ing on Lo-< ot jo’ ( See GesGiry wevte lan preuiouy yee ). 1. joge é, rer enmdid in q [0 xX cane, / form wih Po fo ovy tft retet ca. To £ eben I~ egal uw, | Ase, vied: OG tet of Jobo o D Yo Poe~z m EME Lae, (465 wonies> counted), | Fo 39602 x Jol , 10° phe Pham A440. 1100 om cl Pitter Lo kt Pott tein in 6. for UE TOI! Ale fod Tomer ALD), im Dw Te/o 7 (en eben fv lo, toa ahd: Cyr. q /e@ nwZ Promotion 2% ans : | A) immediate plating | | B) Pact talerded rn bm kl yh, Y 69" fen a0 end of prtte, , tem perry, ! Plax 2's : Ol, & Q0e m wu ft @, : By vairheme ) grey bes Lew. At at rooms Weare role re. | | 2g" $0" 3s) B A | a A B a0 NoW iW We, | Mew He) NOL | Mew we, ime, Out ry 88 I9¢ 146) 26 zz 0.02 23 10 27 | +6 35) | DATE: 23/¢/S8 . REF: [405-6 - 1 Yo 3 4 _ 5 6 - 8 9 10 SEGREGATION, . . / 2 / Mow ‘NC, Won | NC MON (Ve | geht cfioo Ofice o/ioo ofios o/19 0 ofoc 10) Lac 2/ic0 Soo Sivoo A100 7/120 8/944 Ti 25fioo 38/94 33, 37/1089 3g foo 54/09 _ | | 30 40): 50 — = i REF: - - 1 3 4 5 6 7 8 9 10 CROSSES 38 xk 3. | é yo. 2323 ,o6¥ pos Geto re “30 vy col, (tome + G mt from Oo. 2c.) Ragged an b. pediuwm at ! do X done, ° eX Ma ed equal aon andl, Phen HOTA Ao off. 10" pelpe te Ly 1 ° wnedicn em bwecrteted 50", thee Spread. vy main j 4 Sag refle Gb : : and VYig- gure 0.05 fer unt @. a he, Korb ) Awd Aj ilenrp ae fC. Hee din my | Pro portal SSP VS I —? ho Ayt. weter 7.05 ‘8 fre | a i Sy L, 9.08 on fee 8, | Lt 20 | hoo Ly | Y Uv, _ OG fuica/e Fecal facg | Yo by a Jarre. end witpo/repice | counk . . . 42 x 1oI f Micvefop, comdp :| 2323 6: | je ZSosoH @& . F2x 10 fue al | | [ oF nae ' : 3964 \o7 | ra 2-10 [root viodds U3 320. lo = 6-10" /at wot Vie Ce hey : So6y v lo. 000 40 wy oJ 3600 | Cros ny Coke LIB Deane 3064 pardon pal Cdgcy . sal Crosses: af eiwt 3obu, ! (go fra. whe w . | fo r “ { ° to D ihe ' oATe: ASIS ———-curtere | PHASE, oer: FOS | 1 2 3 4 5 on 6 7 | 8 I 10 a ae ee ee te oo fe __- Peat mate tse mh D eum 3060, 4 wt £1G unk bake (0.29 | 70 CY. (art | 0.27— | / o S: % 0, 3 ’ | Ou P. uw, | lo aw. neh te | poe nadie ~ nm. 10 Ayo 2 Slee Ted over asested ) broth tr. ge6e Sar OSt : | 306% Siz, 0.80. redneedy ! Ath bt Tome (to dainty sot be teat 2060 capri sol hey Abt wh Onfh. (2984 POT tos wo, LOB nk + tmeg 3060 Sor FS mts Gwe tre , 36Y Sot Pat nis Km)_ NM:y Rave mw tynnk anno wwG ) ee +. Ln fo WT aba7T : 3066 ser 3060 Exp, 3064 for 0b Exp. : A x | x | | B x x | | co * x | 30 D x ! Ly 5 WY a tue | pervaed Sth, to 09.05 wu JG, reeks. v —pa.0r i” “DYN 5 og, 01 dye for A, care. 4/17/58. | per 6 rr re 2 3 | oa 8 6 8 9 Ate do’ | A B d d- 0.05 482 243 492) 320. J * “f Ys te. lay deve (py weo | WV 1ev0 V0 00 ! After Go! pr ‘s Ct AEs . | ojos weeny frmcranly Jwrtarg) wat] 20 Wo 0-05 498) 3e0 | vSoe Te | 0.07 | wet dn >> 59 Gi a) yo 50 ore wLTURE Phaje UIFSE nee, (IFOT SUMMARY A Ls) c D x j J exp Ap eg 9 ORD tp [4 Oo | Mot o/5o ffs | %%> | °/Se Gol $ 0/50 0/50 o/s° Offre sarc 4 '/So So Ys5e| o/Se 20); [2/s0 gjso 10/se Hg / 60’ Mak tt o/To : ols a/seo | o/se Sake 6/s° 5750 2)§ 9 6/50 Love vofro 16/50 12)s0 &Uu/s2 30! tT 30/s0 3e /s0 42/50 2G /$ 0 50 10 20 30), ao ah A € * Fj ". st phage Lin Lon via As h. phage pas ie ile may result; in a bransdud sional eyant., | igteregenobtic clonas when donor and recipient boa ve adcuun tive recons, The hsterogenotes were uns table, the two input and two crossover classes (dozse : and Ledarbsyg 1956). Two cistrons (cis-teans oad iedon offen’ groups) had been demonstrated: trans heterogenotes wate name vers of the save clatron are phenotypically iatant. Caleeto. Hinase was migsing in mutants of ons cistron (Gal 2,8) while UDP transfexass aati. ty wag absent in the other (Gal L; 4s 5, 7),.8 dsfect corresponiing to haman congenital. galac ton semia, (Kalekar, Kuvahasht 1957), Gal ard Gablg™ azo : gonetlically clatronic with both of the” fo: eget Ng groupe | sy have not yet been successfully a madysed for the: aatie defect. aAgeng two undred naw mutants, no ree ident ical. witha the first tan have resurred. Most he. aasignsd to aither of the firsts two elstecnic groups, Li numbar which would rep resent a fourth ganctic @ given only normal galectasea=positive ks wee tested, A fer mitunts ware nob ey other podit fers. avery standard Gal formable by f but ware found to co. vlnding that of hexoses ms tabolian which cbscure their lonship te ths Gal” groupee-=/in athempt vas made t 3 markers tn Linoar sequence by their rola thong nip Locus, Jatarorosaes of Hfr HW” Gal.” x F° Gal.” wore a fy * dthe ‘a oh ono medium which selected efficiently f combinante, and th:sa were then scored oP an JB marker. H weve the are LOth Internat | Consreas of Genetica, Montreal, Loss. U.W. MICROBIAL GENETICS oU WON DH SF wD = + oO ON OW PWD & ow ON DM bw HD oo ODN AWM PWD oOo WMW ON MDM BP wD = W 390% W 3870 Gy UF t. imtiog Se 1.0 f _ brrerbnbinde pr or PLEA om Phat for G, fy FOS os Yow Carter : oo of md fid enrllorey, wot | wdc l 7 lope Gol + & Gol - Mio $6 Gol+ | qal- Hie0 4 got + 3 gol - Mowe o Co-wtwet W3Io08 3 eek. W 3870 © uk. 4 é fe, How » OVO U.W. obo ON OO hw HD = wma — o May 19 1958 a 1408/3 — a hy Linkage ‘(| @ | 3908S mete lp”), | Ga: 1 Ww 3367 yk mR Mer‘: 5 2A. W306o + . th? | 28, W3t7o Ak - 1h? (A Gat tap 6 im : 3. W3848 si Paty S G4 Jr $.. ‘ 4: W37s2 s | Croy | ; 13): W3782 s M- | oc & +99, 1 39 3 ZI: w 38q8 +; C- 4G A), of of fo” 10, bo? pda ioe to role ne ty é | ' ia? ° | Gehl Ip /gat! — O6'/Guk 2] 4. oO |D ®@ _\|~ | | ‘| 2A oo 5% 70 32% | 26 | a 257 a% 1%. s| 3 ly oy 1B 7, | 3 \. 29 2 16°, | | 2 3 26 es oo yr | Pe 7 ot yam 6 | care. SIS SJ oo te oe <7 ¢ ce t g | NS h { h eg end, ! peel me S Gk fn 8, D( dn GJ Mm Gat prs & Tryp a od be fore et blew peey (& Se $ & e, eka peach em ft mee vs S AS ep 40 Px f x . & WN * oS 20 i8 ~ Tt ah bo | So 3i a1 (TY 149 5 f 40 | Ivo 1 48 Sy gL GR 50 Ga eq fo (ism) ~ . 4 4 pon Bote f Tr LAD “4 7 ot ‘ V yale . s Ly taf * aa pudt. te tend fe Bo fu ee 4of.y po ud UW. MICROBIAL. GENETICS ee 19 REF ito Whee 3 4 Ss 6 | 7 8 | 3 10 2 ¢ GLkf-B, | Corrie] _ 3 aL. a f . . 4 me . ; 5 wt a A . & ; 4 7 qabtltet] Tat qnkey, |oatefeer tot erg By ws & ~~ yo - i 0 to! iffe, 4 240 1h aM Nag mall I, . - fo G4 jr. pasa ain 17/94 WE |) 2h, ee Sey 2 - , ; : ; ; / "EN gy : Jo) fo far | 31 Of, | S/H 149 | 3.3% Bo NO a pF ‘ * ee .t a 7 5 40 8/94 | ur 16%, lid/400 5% |ae% qo 4 7 8 . Vigna 7 so" Y7/27) 423 hry weaany a . 2 i cee ee . i a bo! . tos. tebe : vt . | eo . Tih a 2 ai 4a _S 6 Zit 8 3 0 1 _ 2i 3 4 5 6 7 8 9 fowl ; ! 2 3 : : 4 : s ! : | | | 8 | 9 | | 0 | t U.W. MICROBIAL | GENETICS ° bed tt .. -f Pop on fone . 19 May (6 i! REF [<2 LA a = : 7 ee 1 2 3 4 5 6 7 8 9 | 10 { , a VY eee . web . : . . bo i all WW 18 pe] me, aba [DP ew Att) = I St oe a - be - All We 34.48 “f A} wet 4 | 7 —— 2 wet: 6 — _ a dpe, reguespe dak | Wwrredl MaIn4 na x Cte 9 . . | . . wa . : 0 2ei Pi + tone ¢ t+ Code Ll “4 4to4 te. i t - A | Co uh SB dekle ce ee Ah 5 thee es al, Co us . © | mG eo ee ~ tpt 8 OS nat bse > al To ‘ - 5 2.08 4 64 phePed - 7 Vee ; v nd +, Sy ned Lr /t ~—F t- f y wv oe “> cs ft - BI + a 9 pee gene whe ge . _ _ ail . on hy YELS ats _$ . Os] gh Cuted = 4 - C J eA Caped ze t Og oy lave . Per Go 4 . . a a 4 al feb | firn prety ae ep dy mteas of Yrorwted ge a Y _ 6 dink. pipe & LS pnt ca Chad eee tm, toh LA af amd | ays} frit. Pb ib Oo)” ptoTed. oh 9 | 0 LD fe 3B, Meth) ; (Gk fx A, Auep 2 . 3 . * a - 7 r f on * ° f of ” aN . 4 Tined fe, Qs ve lar! 24% 26° Ld) fo ze $5 'Jal Gfiwe ¢ — 7 / oo ° f j ft , 7 . . v SI 6 yo fo 7 ‘ a ne . 1, yt / a Vole (BU ached, ST 70" Qo TT - a £ : . # 4 3 7 ZS o vy Je ) - 1 . - 4 { ae be | | - 3 ~~ vf. , 4 So 0’ 35" 5 7 a ae 7 —_ . +4, at | ; | oy y 3 tow 4 woh Lins Pode ( een Hy oath if ") , Com tacqeane oan pron 9 det HM tf + - or ; 0 Wea tino : “vy Fleas Ake - Ex p , dis¢ pled, . ULW. MICROBIAL GENETICS i____ _4 19 iC 193 f REF l4Lo /s. _ { / 1 2 3 4 5 6 7 38 9 10 GAL PREUM p (AMIN G Pia Te. wee a > to ote anh ny 4 fe ervd SIS y SGoh _ : 3; fjorr Yom “p> 1VO8)s - Cuthrs AorAa nwA nl A. x ‘le 5 7 : i, Lr 2» Pre We ; . Cot fegoal , Have 4 de. ke wd ( ) kh Ka 9 f~4 . ¢ nL a5. -t f une 2K, ! ° fees ot to! at yok, Ba thed thndsd, akties (of if 1; A ! , MA hot, z) Goat gfr 4 D> 4 5 Comps _ of 4 Sg OF of ? 6 5 8 9 0 1 2 3 4 Ss 6 7 8 9 0 1 2 3 4 5 6 7 8 9 o oo ON Oo MO Pw Dn = U.W. MICROBIAL GENETICS REF: ! Ls —— ag So | OM DN Dh e® wn One wD |! D0 & N SLND HM AWD PbO PN mo pw pu oO MN MH BwWD = [yor wlLico “oS. wi 3S U.W. oe copy tl a . ’ Mey 14 1459 ne [LO8/F 1 2 3 4 | 5 6 7 8 J | 10 =F eee oT ' 3 i a afters . a - . 4 # his, W 3F off . a { wt t UF ut Pow { roel » | 2 “Twel Tipe ive ffih A gx. yon ped, i“ Paar _ Bu a4. woe. oe oe a. - . . 9 BB, Puyd ty Preopy ACT L BGA, ol co Ce wo. | AG . dog . al B.. Rink G ere det tod e ert. Qe, nm foote 3. : 7 4 | wedtetoth - 4 2k | ty tebe ot £3, bw, tr dy Sy oF 5 + , ‘ . . . ; : sf Ch Chad Headed , Pere OF oy S'Get Bit (fort nip Mey -8 i | | . oft . . . _ : i all A. & & 3 ome. Jo + & che Chad Penecnoy [4.3 ae Hone Po 8 bath ped peti FER ATE Bee tea Ypo ee te we) : _ Seam Fate) ot om IT) del | oerl gol ar! | ye! 3 sre wt L z u ( ( ( ; (hn Oak Abe ~ ~ / poe 1 2 Rhesad prota oF on. fe meh way on 4 | | ' Peat reget wa. & - BS el of Jo ~~ e WUSY- + ; wedi y 4 Let, pret OL |e OU Gud (0S tof 9 ay | ° POR coma ~ oO is wo LS Ja IT &o | 2 (A) i see (74 386, 440 684) 3 2 83 (t& 176 Le. J Aq Sei 4 3 163 1&3 241 spe, Yd. 5 ri 4 ZiT vue, S7t. ° 6q.5 13€ ee Jor SU | £8 : ; Kl Bae bac. 136 274 foo 1563 | 2382. 0 | ULW. MICROBIAL GENETICS 48/2 REF: {0 19 | > i" oO a dpe ep eet ce ge ee we ence O- _ ° o . x { ene een ee en 4 ~ ce ens cnt np ee + ; “ey * ~y nt . ia g 7 Py © \ \ 9 . bn \ oa - a wo \ \ : gt Soe a 2 oe re tt Joe ee . 2 | + ; \ | | | cn a . Y, ™” ‘ a { : i 41 \ 4 444» + & L 4 } : \ 4 \ her) oO . 8 q & g 8 o ln A rn : bey - oO ~ 2 la ” re) cy ~Y — -aA 4a +n QGra909 06 - A mW tH OR Oo HO U.W. MICROBIAL GENETICS LL 19 — May L( IOS ¢ REF: ( 4-0 8/8 _ 1 2 3 4 5 6 7 | 8 40 . aa Lif ot . g* Ge 2) W438 70 from frp. a — all WW dood fraw~ pour Ady fretedk oty SSL tee) Pen) eh 1 perdi Zo _ 2 - ok 1 ed AK | y road ¢ Ve pn, regnJ ate col Mokug wextr | Soe Ft iS utp at 777 flare ai bong, |f wal’ wy Fae bbe Wf (SF wd cL hd all "4 of : H Ast . A ‘ Gand oke “ froctte. LET (8) . : ae imesh ocak ce rg ae oe a be all. L a 2 ~~ all. Lebin R | fd ¥U «tad 4G [ley fae o7 K€ nG (Alege ol. a. wo. | th Pe | or om mit £8, J | ; 5 ET reegthinaltyh — Jyprnre oh OAC t+ 46 wet si. : = — 7 OL tf coe fede oo peel L ° . ce ee a _ 0 1 2 3H 4 5 6 7 8 $ 1 oll. 2 | 3 | 4 | s | 6 7 ! | 8 | | 9 0 | UW. pee ce MICROBIAL GENETICS ( va oat | mays 19 4, REF: l[¢o b/s T 1 2 3 4 S | 6 7 8 9 T In lee OO MUO Haw 20 IB,trpte 9, 10 ° z o tbe oO | 3h ° o $2 | © Llo c.g ° 4 9 tke oY S~ Pe bu ~Q VEG harig IAN “ ~ +t Hl ~38 | S470) 4 BR | 2, | Cob ON PH > wh = vy ~ ! fw = ek 4.1 24 a of 4 Pr 1 ~~ {oo te ao sts So DO ON DH ewWrd =, COMNH HPWH He - ~ 3 we + OO ON OW Pwr = 2D U.W. MICROBIAL | GENETICS a J _ 19 Mex LY 1G5h REF | to P/G 1 2 4 5 6 7 8 | 3 19 — cp Gel Arty mie wa oy coe tl _ “ 2, (dae ek (408 /d aeehyt |thaf bey & Ga, 3 _ 4b. wa . fol pots 6 ; _ 7 fre 8 44° | 47% | 66 _ 8 - all. (46 442 | (43 (A) + Yoo 7 of I / . ; L yy 4 % A Cer 2 - f 3) ek Ainnty 10, 498° G7 Se! Lane Fer ahd ._ & ' | S | 6 7 _ Bi _ 3. oe al 2) _ 3 ail. 5 6] a 7th. _ 8 9g 0 1 coe al 4 S | 6 7 Bi 93 = 9g ,; | 3 3 i 4 | : t : 7 | a | 9 - | 0 | U.W. MICROBIAL GENETICS §_ (ud 4 ner LY OCG J oe _2 3 oe a1 0 s| {0 8 ID of veo yl 2a al otk. “i 2 3 77 _ 2 3L Bi. _ . ~ ol as rag 1 42,13 | 64 B 2 | 47 B al SOB s 3 . ° . CWO ND Wawro OP Ou nH pw bv = GEA 47 A 8 9 10 a Ss ! | ° 226,27 141 19, 33, 2 ty ue 4 3] a -h SB 43 40 33 As sf 6o 661 47 337 U.W. ow MON HU AWD oO 08 DH DM Pw DR = MICROBIAL i GENETICS Lee al 19 _ REF: _ 1 2 3 4 5 : 7 i) 10 oo Le : : al D Cop 4 nn, Sn os Time | Sy ~ ab, xxdd Sein Ae | eK A! 6| oo al 10. 6) hot ise fol GY 1) 622150 gi of 7 | IS? Y (| B92, 7tY Y fF 1 2b Sa pte fF tS | yy] S62) BB BF | 24,3 | 2 . Lee + al «6230—«d| «236 Yo o1) S900! 170 -¥ 2 iy ¥2,%0 : _ 4 | sl %6 | 345 ¢ 1) 86.25 zrari¢g Fv 5T oe 6 . . - rh 24, 278 | 3.4) G2ez7l 137 3 1.8 GSE 8 p : oe of 32 | 348 Zo EBB, 2U4E 2 1 2209 QO. . . . 35 | Sl2 | & 5 12B.ee) 347 (3 1 ty S.b7 1 i : al 3€@ | SEO 1 3b 8ker, YD 2B ISS C7 ae | 337 0 4 1 38G25' 130 St 272 69 5 : : si ¥ ¥ S31 202, 5340°, 797, 2 1.3985 7 . - | . : al ¥7 | P61 2 Seg) 549 we 3 h23.6 3 0 So {iyo 2. % aGeo oo Ot 2. ¥K2OdY¥ U.W. MICROBIAL GENETICS 19 REF: F ‘ + f » é hee — ——_— Q 9 af _ So ce ee . weet ee ae ro ‘ ‘ or, ~J n » i : ‘ 2. , 4 e a * . i GO — hee oct + -O - ee oa ee ee a fs > . _ _ 4 , = + Sy © ao ee ‘ 1 eo +. + mo ~ AG ~ ao \ Mm \ tS ' aL 6 « on °. “sy eo. a ie , 4 : wo 05 ~ ‘6 Ne . le Be ~ wn ® . a 3 Pe ¢ * « ease ~ +- an ' tr : ” fi Oo | ~ e cannes { \ ti | j {. Oo Oo ey > om 3 O 9 N _—< oe =f. & . +4 J. otf | : . : ; : “— 0m Hw ON. - ” anagagaei-A a7 tnoOnagaoao r“NwMAtTH OR DHO “AO FT HOF OG O , Dp > | o | ® mo, “ : 2 2 § | ~ 3 + | [oP _ i : i i i“ ! | ! | | | ' U.W. MICROBIAL GENETICS | 4. x <2 * ™ | | , oe = - eee fe ge | Ss | a s ~ a | m 4 ne wee [ o> 7 “es c . - ween ay oe ” ~_ © > mm 5 Oa ms _. “ wit ~ es - a - A 6 — . os Ye =~) ~ oa c wo my Pp NL ( 9 Thee te Sy ly wo a a nm: Ne, . ™ ww “ry ¢ ef j 2 4 eu 4 Oo ” wo j = a sv nv | > a a a ~ - as $24 + , a lil e © -~ NOM TMH UH OHO -~“-A OtwMNunhOagaAaGA -~ NOt our OHS -A OW tH OR OH O UW. MICROBIAL GENETICS ad 198 8 May 307" REF re Plo. 1 2 3 4 5 6 7 8 | 3 10 _ ap Oak Rag - ff a a _ 4 oo . _S W 3870 ki Ww 3459. (GAL Try ) - 6 - co ca coe a] Gane og [l40d/e| aa) 1608/9 - 3 GRITS tt C1 de 7S J 2 8878 frre hk bf > fry WEG pean Fota hed | ort Ye) _ | 3} ¢ | | : “i Spe repre hd he pOwnre (wey, . hee etd J bk it (Fad 9, _ 6 a fenr.~ 4a Ot they 2B 2 8. SG +S a eB OT ELT at ol Fro 4 dmerat | ote Leg PAGAAA ( G f~) , ; . ay 4 q “7 Fag} SI Io ag - _ alent A ‘ty Yr Loc Lor Lee Vos ‘he Lor bbnd ¢ hid > 4 ae 2. 3 . £ : I WA OR si Fe Lo so ov ACTA ~ | Nemec te 7 By 3 0 ou py Dm eB wh ow OWN OD HM PP Yh oF” f i re LA~ a & U.W. —_— MICROBIAL ' oO DN DM PWD = oO ON DM FP wh = ow moun wm awn = GENETICS 9 F759 Gel, /r 4 * a & 39% ar kt bd, o8/ Bees 19 5% REF: | t (0 _ a 2 3 4 5 6 7 8 | 9 10 4] Di 4 - "gio (yn & ; | : ; fen) Arrest guts ib. Suen Ave. | Saf of 3 a 134) ral | 3 | 4 | i | i si 23. | 6h 4 46 i523 5 3 Be Sh RID 6 || { 7, U6 C2, 98,74, (122° 3,9,0 83,4057 139 707. Gal 8 | _ oo 9) U4 97,63, 30° (6 We Gb 4 6. oO | . 5 . (oh 196 (27 #3 55,93 | iad 7 | 27 aS ja sus [30 4,7 (F499 | ‘Ft 9O¢ 6e/ 38 |p30, 28 GS 20F,9 24 Yo SO. pe. 298 NG | 7 SB) Bt | RR TTB 2719 3 [3G fK osu | gaz 2Ws YP 273,%I7, <3 735 SP WR We ate gee 77 8 USS ~C 1530 Baas 200, 119 ee) Ws7 | 43g So 6) 251 me tD) 30% (BEGG Lh MP 209 SY aT _ £ x | D3 B, 333) 317 XW! Sea EWP UP WS, HHL) Ue r9 53,8 1& | S7os 52. f by ‘ — 4 90) S79 rE RB ey 24 % TO) To gyo 47, 7 72/39 | 59 B 136, ~ too 13 Gov 72,7 143 ive % o om \f | ( J ? cotr~I od a! gve ovo Own OO MW AWD | Weenies ons bi nawly Vy —_» time |408/9 ecan Gal, [408/lo_ 280 $2, Expts : fo 6 og . — - rend uwselh of Gals bee) 0 ] wu 4 2 a 1 - vo? ° b 3 _¢ ge PM on tte ' ; & ULW. | MICROBIAL GENETICS 4 19 _. 1 2 3 4 5 6 -- - Aan r OET / r ~} fo pom , 1 MA Spe Le es Sf oe eee ey Bf & : é L - | ‘ EC. 4 a5 COLES Ang f. nga ow tat zd Ad } ATS, s¥ ] deo 4s ’ oe & nha oe i wt c w& } Ct w Aialie 4 Soo “ Qo, The Galt (odd) | C hte pan B poo Lag . _. onl, : wiyy rly { | ow ONO MN SF WN — oO woW ON DWH PF wD = 2% YQ 122 xGs J 24 yor / Ay 87 ey 3 pa fa? 34 3,° ryt ity oT = en aD. an 344 ap . 9 Jas 3s - 1F to 1 ! . a VG wy ai 418 oh ay. a fo. al) 44 © Re ,o 5 6 47 ane SOK 7 . . al JO» ® 128 9 . of Y3 3 k Pe as Whose . oi Ur / 2 fe ay ays wt, co [!03, fo 4 be ee ED / ‘ . _ 5 © is, . 1 6 no a + yt og ay l a ut A : i 7 EE rae ST as Ge Gat 8 Ale £ i, pe TO 9 de t o fa | . O “pape etre & “y 1 oe , a 2 . Lgetad eet 3 L ene a wat c * ia? is is 's 4 5 vA Sen G2¢¥ 743 G4 : : wr | 6 32 - iQ3- Bet 6 G5 PE - — 4 i 7m yk ~ Ge 5 7h 8 <4 - ~ a3 oS tf 9 t 0 i i ULW. MICROBIAL GENETICS 19 REF 1 2 3 4 5 6 7 _ aoe ( ite arr \ Ane ji we peter 1 ! 2i Zot f ‘ r —_— 3 , Comey. ‘ We ‘9 t | ee - wl ved, ah 4 : 1 i 5 | | | Ore pe TR gett byt A} 6 v tke ~ j ? . 1 i 8 7 ON foe - i 9 7 . i : 9 ay PFS ~ | Ver _ ‘ : 1 : / oO i © + 2 i | , ~ ' 7 az! / ry a { i é 4 _- ae ; 5 of / ous 3 q Z 7 Ly “> 71 ° aN 23 Lo ahe B : - - yy yt + . “ 9 f Ld 2 ts Jy » 2 06 o a a vy [434 2to we hb 258 1 ’ ~, 7 me 2 “7 YR RAY Qo war bo: 3 - , os ; al fo | aroed | it te | vue Lage 5 - 6 aos F770" roye x & 317 7 —_ a m4 326i ge 4, _t Site 9 7 ” 6 i i 5 s agr ; tec oped FR | : i . . Voges i a * 3? . 4 1 ac ‘p (ented cabtend get pared : ( 4 t } XN 1 Ly % WAN 4 Gand “i jad. y 2 Z ~ ‘ 3 4 5 4 7 8 3 9 | i ‘ | 2 2 | 7 ( 5 7 | 8 | 9 ° I U.W. MICROBIAL GENETICS . 19 Peg ~ rer: ft) fi { 2 3 4 5 6 ip 4 pf “ho te ys 7 + F +t ads y La Tr . ae thes Pp fet , t Speak #7 hyp me on: , o ‘ * 1 i +) xx Here 4 dese . reed ‘get ee orn 2\t : 2 : ofa ; : E xpeinede : ti 2k: \ PEACH: om Xe, ak (40 bf z loo ‘wo ON OND GS4NIae "OO N39Z1gI0 SN3IONG ° SNOISIAIC Of X SHTDAD E'SINHLIGVOOT IF YadWd HdVHD NEOZLAIG OIET-OvE ‘O} ULW. MICROBIAL GENETICS poi ot +r + 4 + + U.w. MICROBIAL GENETICS wa cae a © app ng etait ree nnn PS tn aN ed hig ana beg asd BS FB A icici ct ULW. SeNerics || GENETICS 0993 wCS MONI G3LNIAS "GO N39ZL310 SN3ONa SNOISIAIG O1 X SH IDAD €-DINHLIMVOOTIF HadVd HdVHOD NSOZLAIG OIE T-OPE *O} ‘ . U.W. MICROBIAL GENETICS ULW. 14 of / MICROBIAL 14 06/16 U.W. MICROBIAL GENETICS U.W. MICROBIAL GENETICS U.W. MICROBIAL GENETICS ULW. | MICROBIAL GENETICS weerter pie prendre fe pet bebe bees steered twoB ON ON: GaINeaa SNOISIAID O§1 X SHIDAD €- DINHiIeVOOTI "O90 NaSZ131G AN3ONS YadWd HdVHD NAOZLSIG OET-OVE ‘O: pate: 4: [¢- Sy, REF: [Itog—-I 1 2 3 a 5 6 7 8 9 10 ENZYMES) ON). PReTo PLLA [T/_ 1 awk ~wt of Rofo +170 ~e Cy + (0 US Menethin O.F quoat ( at q Jooy ot a ‘0 Commdi pad, Ven tangeanchee ch a hat Pha) aanne AT 7 2). Mixed cn | tonal whammy wiht: A N-afe 2 wp [We ma fy B c oT patin, te C tig % 20 D Du- ase ° Nt] contet 4 Onde, F Viwgeme YY [0.tr at mde roa tret bh + areal © SG sey serygany O.ur me + Vege Oar + |o O.cr, Afi, 15-36": Pabplag um n Peey Rafer, (niQny At) 3° C20 sinnkt pqewes Spans, wal iy x) 10%), W- A B cd {DD & G 63 Jo 60 5¢ Sf fz SF, Aha 2 Rooters : . «4h a7 at | a, 42 63 | etn. ey yl Cord Sev . Orly RWA-p | fee | Co dypeT and or © af reo tif) tee preg tL Thus oo tee q Lees of plight | by, Adef ran vy node. jf Ao! then OT wr, adalk,/ % RM ay u ON-ey Cow rb ' OG, padanw Va cen ‘ ‘c - CT an Hn Dlot wt o win ft B, Catton pont oy Heaps ted foe tap. 1406-6 a Ln Tyr. fouls | us « A to ” ¢C¢ | DBD L 0.1 |. tor rary , | = oo 001, (/3) 43) 62) 74) 923 Godt | 0ff8| 2/43 off our} 1/50 lnct | 7/48 5/43) Slee 1/¥2| 6/3 o =| 1qfaf 18/43) 31/44 16/92, 23/50 [40 DATE: 4/18/58 — REF: - [410 INTERAUPTION AND! pielorns. 29U$, UTR | Came overnite pote Cte, en wee - taal Arno wally pre q 3" thor RETA Yo iw Wwrorus batt. pr tn enn be-Brrn ~ eh, t y bolls wet Ah Af foc tomy : 7 haphats . 0.7 > Pen &, i! Slee we-? ho Pl > 1 7: btne, She, Pr? he Pp. 97 ~» “6 mT | YB le. V.2. Mowdres puranacnagls ne preter wt (evra and L seared oy bec, ‘SAC yo' (Bl Go'fe (B,) wey 7° ans ) #4 286 | dr | 381 (i tne) Sha, = | 929 | 2-7 | 6g ha bach Clb, |3de92| = Bere Agbag- feels Abas + sae i re a(t) pking i bog phen DATE: . 10 tof —— 30f SF ee - : de cee eee fee ee - | le bey 20 30 40 50 pate, 22/4 vcr. [40/2 1 2 3 5 8 9 10 Exe apts tod . iYle/, | repeated. PleK ng g 4 dul ik Dw A Oot oh win &, ©.t of SiacB, KGa 8, wwe) 2323) ? /o Lacy U.W. MICROBIAL GENETICS DATE: 4/21 [58 REF: LU 20 1 2 3 4 5 6 7 = (—CouKHICIME- | Efget om anak: wg Ftd a im ad orkdhcam 1% Mm AyCbhg wrle, | mann steele, | sted ef) -20° io| % 30K 3Jo6h overnight T robe [alt ; OL wt te 0.8 we Pemroto, + 0.) LO. Cortreey te a + | Cobelli! wel rn 1%, 4 rote K gw . dp i et Keel canal annou~ty vf wtehkund broths + OR eetehcce font I and Kak. yee enn wd ¢ (Covet) brett, 1 O.t wal wont’ [a ann Yeh teal ermewK_ fC ) Getty Onl wet wet ey 7 Ao'| imncotee wm wel beth, then sak hen wm Wet ol thon K, Dw — 0.05 | minst B, Lo No Dw +5 Sawt | Wh Dw |? 0.05 Blas | fo | asstes | |g ume “ao fio hice deact | bac = Iso 6 o (7 rr , 6 IY —¢ (46° il 6 | 15 __ 50 Condery ns | phe | pels apr a fool decree | Ne. probhas addatap lhe TH pel 4 wi x toy (& wet loub oOOM adler |e MH ts IN od Sobre Fethece ) v\e Ye 5 U.W, MICROBIAL GENETICS 14j2 Bobo x Bob XY LE ane wr, [t(2 9 10 20} 30 40 50|| | . O.tep. % SG Le i for~ tyr tebe . be (0,1 bake + 4.1 is Soe fente Cie xe) peo) > O44 __ 30] @ _ tee De Co ter - : _ a frit ee _ _£ 3/F - Ebel lait 72 . Se Et eee Z __ 40 _ Po, ob _ ~_50\) __ : ee - _ mop _ L fo _ | Ob 4 od REF: (412. 10 — _ oe _ 42 OF a MO Ov! °.05 ced. oe : A4-A | 3 | © - - AsaAl co | ol a _ - Ag B 4) 3 | | oe _ 7 begecact ote hb _— zp | AaB 8 too 7 — a Ay | 42 I> [oo fo _ _ _ L Ay Cc eC © _ a Conch: Ons _ ee) eee eee ; - 40 I Po 7 - ; a = 50 | L | - fp ULW. MICROBIAL GENETICS ULW. MICROBIAL GENETICS + t > Pett yp beh pefial U.W. cenerics, «= J) E BE fie U.W. MICROBIAL GENETICS EF. (GI, U.W. MICROBIAL JUjQ GENETICS U.W. MICROBIAL GENETICS JY KLE-GBLE- GLE -BLENDING - pyc -(NG- DATE: G/2 C/ re. rer: [

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Ward: Uw. | ld MICROBIAL we No SS 1 ‘ GENETICS HY) itn, The Dee ~ T pacers puss) vs. Prapeneee tony) | bell for-_etep~erey hae. | corked soak é v ah, | eee Gi fone ! a’ Sone. w3obeles p w3e8 ¥/0%) Bh AM ybtade Imink Ose BL! mnt war beet ® —Valp in beat ees Rrra Gx, A AAd Pt 10°38 | Btdd 7 chill bull C Add i, elubbd Dla ll 3 elles. | | 20% tacos HA:B DibLE( Yim) + pte BA. Dep. CA, Dep. fo: Aas nonin tp. eat han, CR At hers ec Pad rectate © hone dilulé, . pies toma ne CO 2 ON OM PY Ne, BGP Ye Ns, on! SD. Aud. DAP fo 2, Op net Hoel. | DA. taht 70 muriatio , hp (Hund?) DO Prelate raids, dre 2 a , jeer. te, bth = alts tpt 6.2 " B ‘ 6.30 - Lea uv! CODN MDH RWDN es | h wot top cnc ft OW ON DO fw DH = 2.8 PaO ALP Ror U.W. MICROBIAL GENETICS 0 May Pas ae I con eg AS Bg no ee 44 | | Protect dkending 3060, 306k, fone. 2% and Fo x Sp. : A wt tal, A tet phe ot 37° add lo nt BEA wa wad, de bacon fete (se mS BGA, cent 12 mei males. A. whet Ol +44 ile, bend 4, ole o.05 om usin ft &), : B. : " | n BGA won, mente fo’ bend a plod , © add Dwe (dimtrophemcd ) fo. tscndeta 5’ Chud ; 5 | Cy: remove forugks f Ot, WEL Hema d peat. 2 ! Cas tebe 50! Ab tlk ype 5 | | Cy rn cota 30! A bX, enen tale 0" Beird. curd hy 2 Ds ada DWE Yoo, imentole 30! abate ty epient Were 664 7 De: witeleai jo pe 4 pak. b Mr mate tr. _ Finn AL. wee Tut W,. | | A — «6B Cc, Ca Cc, D, Dz ' : 4 4o 8 O04 00 0,0 1,0 4,0 eee : Cte her Welt tly ans + ao 7 F - AICO AL Pepe Man 4 Wc. GENETICS —Hitey. £ Meg. wot re a ner IB I2 « pele abso w3e70 Saninuli /t my). Diheele #1 joo, Pasabate ni 4 ‘| shin coy! wd slot 32° bt Ion Pusll fc) U 300 nh, A A Bl sare snd pt (gle = 1:10 dale, pi bale wats red eek 8. Mrieadhete andl file ol 6-60. — C Fbatohed | 1306 . . He. Te W067 df. end Wir Male At > =60 1306 = BPI UY, Serie, Mag 37- 4 A nd wl Pate | | : # aes “nmol OF » s tL b&= 24 : | | al t =b0 | | 1G a at Bho, entinnia soul, weno) oY fs ewe Ns. Ae enleg snub] by FP ee? ? 14/48 it ute he ibe Lh dig pte Soby+ W367 (ont 4 mamiak ( ht=4 Aalats 00 i | (4 els dunt. ( ). : . “O04 bte/2m. 3 oh. load, sanuple-ael les tym nigh uct wbpuel 7 at t= 2/2. te Gs le ME in trnabs ot Tons ) B. ladda le Plole ot t= 35m. (receranpl rratng ) te. w2et/ 9/arbero Wd) We at E=I2 . Pate too feaze. e ( Wb WY cioeerry 2D. A + Wb (= Swh ox ORC Wb pte 10 ind sersagele), Plele Ft=/2 C Do); ed Oo Mae et t=32 (p20). apie tes um tea fon rn nbs’) G) a ssa enon “i oes fol ims Lond Walaa ™ Se +t tae ™™ 51 purl Lae Und 5, apes Dai Pogo te Jo beu® 2 Moy Wee Pete <2 wvduceld bid [he ml hd a mony eG 4,600.0). L414 %. ULW. MICROBIAL GENETICS ae 4 REF: 3 LAYS Me. Yu uJ . uy & : ” vie ~~ ! i . 1 | wt - my ow N Zeis. . . ie 1: he 1 Tn Ge ln ole eee , nO OD aA Oem SE Oe eh} -n mtn on Oono “NM eH OR DOO - NO em On OO + {0 SX (414 B D 20:2 REF ee nee D O caht balm | JS i OX a5 . wove . 3 . fem panna mmmerteinamnnesensninteatanenman sen ah int 6 v Lt . B, May 719 €§ U.W. MICROBIAL GENETICS ~-a arn onagadg ~An tH Or @hO vat _.84 9g Qt 1 2 3 4 5 6 - FH Bh StL Q “Nw FW OR a ao “= NM tw ULW. | ee MICROBIAL GENETICS DP 2o 10 REF: 19 Pye EE Do - verreeo Y20 AM how bac tutic, No Wes Arve Ze (1 : VET deape i dello, (3) lesan o parr i lmagled Hp YF meds volun ge F | : ALee Ob Ft t ) | (2), | wg OE I takin 0) ULW. ee MICROBIAL | GENETICS bo Man at® 1458. ; ve ISB { 2 3 4 5 6 7 8 9 10 |W 3060 (4 1 wee) 7.5 we Penaryany, 8 ht 3| W064 Tut } . low, s| 3°] retelim. Sp im wot tind powded hiftnT 7) entarag,, beth bute i sprat ome iy S| tw | Haley The rangp tn 0S mk ( Comtentetry D (a-speKe), ¥ ond Pew | S Tour | oH Dlospore. a : x : DM): 4 of 20 %y ylucofs and 5 Ajporicacd. gi F.0, 6 ! a] Aaide, avd mecbated aff 37° fx o| S§ ‘ Thun: : | | 1 PanpenK i mawmr oaide D(a5p) wixed | 0.2% + 9, ¢ wl 2 , 4 3 and wthred | for a> maantey Stn A lanteat OF pollen; 7 ; joue + : q i% ln | 8 S ogide v a | PardTe cn Dloip) agra 1S % Orme FOL me, psa for 2p meeety, Hen ACTA a fotos 2 | | ; = OnE mM fo we D (esp) ! 5 | : 6 D. foume + eqide 1 wee +f £9 Pe 7 | . ‘ | ° CE) Unsprthsed tvowtiir obi | onthe /e ‘ os lit , de » ame. | fo (0| mh of Dfasp) at 37°, #°. Ds tnd Jive, pew 29S, : 6) Un, reed. Untel jo’: ood YY. wlDn Z ; cart, per fo Jo wee 4 D/esp af 27° 407 peak of 6 i | : 7 | ! 9 | 0 : | U.wW. t _. vcr. VSS, 2 A aud Cc : after 40’ po pre, Al Kos in ‘ wetter, plete 0S ! win [mn G, ; B aud (D ; D i wel te t ak. | 8, DL: 4 me tr Dap) fo we Fo! in 2 welt tate, Meo of” own mia fir 8, 3 i : 5) Plate peclomtnnn al one | | , [o” ¥ Actuke. q porevtat Deperriong, (or j ent : dni frn 8, plate | | 2 . egreeefoTed , ; pres : Joo 5jo Y, 2240 kro At 5 L | | SoSH = §2% x Br0x 10° me 7 : | -7 10 Aiud, of on B lac ¢ ' | 3069 loo, 139 | ! 3064; 159, (64 : : le, . Dri tinal ten. weniny : Zh x Lg” Joe, j Su vio "fort 5p 8 | ! ° | i 1 : 5 6 10 nce [CIT , ee - te, : ee ft } os Ss ee % ae ~ - _ ~S < $ eg £ ; / yea $ $ Gk, 7 i L 1 { . i eee = “ j Ny §Jro 250 /4o too er 19 omen L only. ULW. MICROBIAL GENETICS mo Fw ~ ono NO FM ORK OHO -“- NO TM OP OHO ~-nNOMOerON VDE OHO -~- nN OTM UR DOA O “MN wo UW. MICROBIAL | GENETICS ee Mag 1S 1959 ng AIT 1 2 3 4 5 6 7 8 9 | 10 Atioeg :| ooface 2 iW s060| pam fy, Let tirv mae | ta det |p Spence Sx hn lw 3064 4) + 7. fae Pew | un Ja ; : : mM Psdvc.me S| Many eile | ot at elo et, A ple “ect 2 the [Baek “ Punayas, ba none fer covsttey (4 C phe, 3 6 4 wk jadbd | ben Hel Por. | tp OL ae f Ayca. wottty | ; ( 6A 8 9 ' q ‘ " Py fo oxe Fm row 42 fhe Jaa care rf ted nd wy fue of 40 2 3 CAD dA s “p z 2 ¢ h- 4 7 i 8 : a 9 Courts ro 3. te « to” pow to ° oO i 2 3 4 Ss 1 2 P ( Pustiey hice 24 44 7g >/ov >f00 3 4 5 B ( boa) g i a 13 ly ly : alten 5 6 7 3 Pi fle | /8%) 3°) 8% 9 ale | 13%, t3% fact | SOD 3%, Wo) 437%. rF, -48% ' 2 BER h+ | 7c of ofe of13 oft 41s 5 Macy | yo. of o{2 3/13 a/y S/tY | 6 | 7 | 8 | 3 i OQ | UW. i MICROBIAL GENETICS Le ne 19 _ Moy 03/58 | REF: [ELS/S. cpr nett fAroe, aNef 7 | % . ? |. * a W Boho | dul pg) Dr e Men ¥ die oA 3) W 3k ke | OF dk e727 fea | Lacabele (21) Chett) fer (ol > Ma ZL eC, 0 f- | CMW MPN DH Aw Nn - Cf ine 7 QNF M/r2 = Mi Wwe o = —™ ce o C ~ 8 d 4 a DP ON DH PF wh & | + + T pa be be seeder, OW ON DW PF Whe + i } 1 bop ' : po Poe i Poy Sp po: | : pod ' foo: : | od poop i . . ! ob ON MH Bw he , < eee Lg ceuhe Oo 080 OWN OW Pw DH = 10 | . __. oo _ oe : | - - i _ a | | ‘ : Ln ' oo ; ; ~ t ne oo ; ~~ ‘+ i ~~ a | i a ; ee Lu ge; | 1 i , ~ { a | ~~ | a ~ -. i ~ ~ \ : aw | ~~ i ¥ 1 wo | . ya | , 3 . a 7 TS “ : 7 “" a at 4 . 4 i = , 3 x *t oe | - hy ioe = reer UA | ” > co a es a ~~. o . _ ee a oo _ ieee os poet a, e ny : . ww ny - : vel ry 5 3 Oo ) ‘ 3 a :> o ¥ ~2 o ? \y P e 5 “" fo *s © a La MM “4 La A mh ue - mh val ~~, ~ ~~ ‘a LA we a ~ ar C nS t NN ~~ OO 9 re = an ethe NO a > m Ga 7 ~ ~~ S . 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L G Lig wet G2 “eo fhow ‘ ta wal mumbec | = mpg of protheg Be Doman errridrotly “| cme pel band a 4 vebindy ~f Com F 7Aty Uw°tre. 2 all tober thor Th “er te 2! ! 1 3 | 4 s ! 6 7 9 | : | U.W. oe MICROBIAL | GENETICS Lose ene wp Sfrb [st _ eer IFIS/O 1 2 3 4 5 6 7 8 Y | | AZIDE RESISFANTS. > : : ‘ W 3efo » W397 £5460 Ace) t) W 3064 , W3935 > C3964 Ark _ 2Z 8 - ber ~ 9 q afta ey Spar, reat 2x dere. | Mag nasil Lah HD, Al Ss + Ps vg iced fuspenpory 6 Sst er 2 | | | | POPNOW Ph wD | CH PND HA WNL oW MN DON PWD : | D ori+ £ r Each |wixlar ayy fTih fo & on beg , Jif ~é pack. | FaacOry | ede 1 Of mt | Wrecker / 2 Arh 8% O.lu€ of 0” 3 ett a 4: 4 eT LR | bof kOe O 7 Mek re ded . L At fol heelys i/S- Abed we Hhottety, Hatry 7 D(G-B,). owe for Of, ATO fe HE he | | _ - | Y | | po | | | | | ho | | | oO M9 ND WH Fw HK | ULW. MICROBIAL GENETICS 7 Luo. ~_ “Sead o a a ! oO “ Lip. xr : ~ - —— . ee | se te A, aes gl r 5 op ‘.. a 2 2 ia aan ; ; 4 ON \3 tT ~ ~ Oo, o # > 7 we al ~~ , yow ; ~ ‘ ~ — ~ ed ’ 4 “ ae a Z 8 W Se Ar goed 2 ad 7% we i i ™ —. * _ od < Soo ~ . - et | \ | d tok m= Mm NO OO OG 9 9 ~ ig _ . % & a fe ‘ to oon, peered ‘ 2 »P 2 Pe Te Oe TN Se NN {jw -~ 5 J “d G Rae > 3 7 2 a ~ 2 oe y Ne Ne ee . ~— ™ J qs. . a = S a q ov & ce OVUEA SK NOM THOR ODO ~AOM FH DN OaOHa “A mM FT nNOUR DAO -anamntnorRroaoad - AN OTH YR OH OO U.W. MICROBIAL GENETICS : aan i ~ & ™ 1 } t - g t re i) - Ww £ 4; x oy * . w z me - saad . er ee ce meetin pe ee ee ee — ee eee a ir te As a » of ty a oe! 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OPTIMAL ALICE WHC. 2 3h . | Onc lrg | of Wis lo gosy 2947 39¢95— 5 | . . / r él dpe ans rey peed _ AL: Cad je woe hott 21 a] bay o typ MIS/S Thee 0G of wip fa Ee ° x OK bn Pap / (OG of wx br fo Bly | to bret of bg ‘ ¢ ay: 4 ud | of pollewig core fag co Receipucg. 2 Wire adda 3 : ; : ° i i a $ Yo | 4 o3% OV Ls © 5 , ; : a} Ca kQal A, 3 eS 7 ; | \ 8 Pan . ° t 2 9 Ay» ( wore thew A Orn fore, jeogye of ¢ es” o - } ™ D(f- Bb). 1 i ' 2 4 2 i & = : A oD 7 st 28 6 ! 7 B oO | f oS tg yy 8 | | . 9 Cc. of “2 | 196 ~ GOO Dif tee EB 2 | t 4 | | s : | 6 | 1 3 | 3 Qo 1 2 3 4 5 $ 7 I 8 9 a ° we DvP. ——: (41S f ud20 —— /Z rete | | See oS 3 Dae evessdbie by Y brute + BO pls - Mind + ple CA) atl WP Mond. Uebel (is tw?) | bear and emsld 6 7 p— way Go. — Fe ree Gf Le Se ae | af ID) 4a rp. a — 2. af 12) aak OW Ps Prebel Bo murites wi YP? V 3. hind + ple, Delile (Tormere Wt), theebte 30 eceler — ~~ yrudrte DAV P D320 miler 5 afta Simul, Yard Oe Ce Sonat. es Lo fhe an posers plete fle bo wy: = D A -#A-t-D. at pe I pik om ryecbe 0 8 BLE fo, BEA > loka K pee 2 ay re TU. Bobo - Book a nl (ro ALY Wa W3 - per part 12 ong |i, Lee oe omy Jnl Wud ALLE lox tt haed Qe0 Us arabe, ad ID alle tach porn + Qh vibebe anti | wd le Adeke tL «FO, rrr FER | fale 10,00 gn ZL seen f?, 2U £0 rupee ee a me a, lel d Fon.) buzone | Adbp Able |) lap ) nutele BO pur Lee Hf oe-D) Thebes. Jibead, 10° 0H rmnmedd 21 be ciHnand adel (ool of hdr, Werner arn proodebe ULW. MICROBIAL GENETICS May Ks SK REF: 1 2 : hoot oO DON OM wn = oO OMYNnan awn = ow ON DW aw DN = ow ON MD UW PWD = oV ON nn pw bh = [I[- fp ‘ab he sal Barkarard B, ov: os OF if Quer nb | j~» las 1B a anh tn. ue La «- QP Crk 1 bee aaG f lo = { UW. MICROBIAL i vere. (4/5 B23 1 | Cote) Gtk) Late TH ° ‘ in oe | A | 88 | 2/88 15/8 si /ee || Ao) 7 | 7 7. 4/7 Condvol 8) 2A | an 3/42 17/92 37/92 "| Bol 2s | ofth, 2/rg 8/eg . / Al ae 3| 28 6 off | of€ (/6 : g | 4/ Af 2¢ Sd | ae fs AE c/a | helene elo. | 2C | 40 | 1/40) 2/40, w/e | : bq | | D g (9) 3/4 6/4 \ DNE 4 LD { o/t 0/1 of ! ™). 2 Q | _— _ i 3. q J V Qo 2 Fri toad bY ET tube pe } toy jp bt dd | tL ee - ee _. . oe iW ; ae =. eee —— —— ae iv fo 4 4 = fro (Qrlink Es ny Tage bor/iro Ns avert (Wart: % Pimnc.4 aye wie, | we sPeeg | rg (aoe pote) Pg obs ip); Para at [oticatcochs parr py tnd dears meter ) bbe, pprevbde tpt. oft Ale wAe-amhet es patno. Prcdrts ; Phas ite 6 rut | | ! s| Tusta CA then. | Moto, To a) andi Tinks Draw pee Mote : A lla! Vihde ke BOA B S prubek | heres , Alte We a, Put «| Raced Co artery lao VR aerials 5) (U 3a¥e 19804 o fd ORC. "Tee bbe je Od “say CL OH. “ota j- eet ante Tab 8 Nk ac postr Lok leh MYX rg ae (anne | yubee) Dilek Ihnen wna CA sph ay bom. lem stab Lil tty (Pat li brelrte jo - 7 L:A Wc, D. | MICROBIAL GENETICS ouMU ON OH F&F wWDN = owW ONAN OU BP WH — ou ON O VW FWD = oo ON OM Bw Dn = oU ON An oO pw Dn Ne a Bl ass Md at tox). nteg oF 37 Aten re Seta [wane BEA andl 4 wns fod | ' i i i 82 San. Se ae BT TAT by (Lib /:10 1, 1204 fo: ley Ae 4 Hi | O./ arf, U.W. MICROBIAL GENETICS \ 28/404 19 SA. L 4 750= = . 5 at é 2 ou Ou onnaeawn = 1 2 5 | 9 a7. ,|W3e60| Ay? ‘ K 3|W3947| Ay 4 5 A= hs g Ss 6 7/ B= oes 4 R 8 9] Cc > oR 9 S oO BD * «PR \ 9 %. 1 2 3 it 4 A fe fo 5 6}. B " 7 <% aye os ; 7 r all ¢ i) / he 1+ 9 f al D é > 1 2 3 4 5 6 7 8 9 o 1 2 3 4 5 6 7 g 9 0 UW. MICROSIAL GENETICS 19 50. 1 24 How 2 rw CoM WN DM S&S WwW DH = ow DON DT UB Ow ON DOU PW DY = NO MO pPwWNH — 4 oo @ ou ON AM pwnd pasal P Mile: | ut a t.¢. A 3S CAE D AR wy chad /0} LAL- oh ~~ Dood VERB 10 “ng Sur SS tee WA D eves be pine U.W. nn — 19 [ac 3™ Las? rer: / _ mi DNA -LEAKAGE 3 : | 4] W30fo w3064) rebtrn poten ow abit Cut) 8 Lecce | / FF unrklarrg carh. Tl Suapedte 4 BGA) IS x aft on tof ol NBC mst tetandad bo mtaty | at Ge Atimndsd. romty, - : BM . han | rharednd. i 5 Gang of Thy madatea OF-8 BR 3.0 rf Aba Dw ca] ty be Spe ! a| CM): VY +S wb; dnen$ 37° for 10! —» Crdad 2 cnc,» "| ? wee be Ak 3| B lad, Goud + mes 37° 4 69 " fal . J , s | tm Him 60 | foun por ¢ be 9 Linwe Feito, Peundad befor sy ried, Q £ | vb ¢ l 2 : | tina O vo! ¢0'S : 8d) Jone o> Be, AT wa thunded. Joyner Ath Meslay | Viets 30! _ "| No ; a oh ph DIVA in . te, aa ape tt ~ th ny P fe ae pant q DNA Pott 4 OWA 9/99 | Hee, 2 ye 1o"” ah, MW ia ruck, 354, SY fuk Jet Fa Es oe, Cesc ¢ 25-15 Hy/ we, | | | oO 8) ON DM pwn ULW. MICROBIAL GENETICS | | fo; i PoP CUMDND MWe wn oP b ON Dw ew CUMPN PHP whe oW MNO WM BW oO ON DO Pw Do Zo utl read uw Lx eek by ban Heed, pans shales ‘ vests, Avo bah of ©. s). ULW. MICROBIAL 10 wi J. . — Fo. 4 i zy a WY a a 4 ! cna | 3 ° mls yy v tle a a TU 4 i ~y we fF ee eh _ — $n et st — —_ —. | a pp rr a rr er 0 34, " @ ajen Srey ' 2: ~ p7 ——— - 2) } wo _4 Ww ot hs oh RP PA HO RAT LP oOo ODN mM nH Pwr — ce rm nal ° “4 oe 2 Prcey Deano e¢ od ~ Sram Dd eowmrnnewp = 6 7 © ODN AM Pw DN Uw. i - MICROBIAL | GENETICS Fo = 19 ee REF: Lad Bey myo ot fader pe 2} | A. 4 | an SH ! $9. Gf : | ; 498 : 5 46.9}. . Berd tf a 2 . ee 3 SS al. ; §3 5] | wee ee nen B ! t — — a7 { - ; — + x, } CO OVO aA wR He WOW PN mime ho oO oO ON DW FW DN & UY eee t+ 441 O92 VP one. (Fo “ (Bases MAD a aae eg N13 aad vt UW. MICROBIAL GENETICS 10 _ __. mw; — Ce + de 4 , . aeey ro T ap? . ” 4 vf x ly Moree ~~ -~ - +476 ¥ / o - — wr a 7 a | : my — . { iy A | > 1 0 AF f y a peep t- j 5 . Lit - . TT ; os 7 A yy ~ a ’ i } nS ho, j . i a j 1 q- peaeri 2 a an 7 * Se p ' bo. 2 co 4 ee bP ° é , : ) a io ° 9 Fal 4s y * * ‘ } : w / o 1 f 7 f o J 45 / NS _ 72 ¥ + os ¢ ! || i ° Org ° ‘ i arty _ LA + + 4 3 t 3 o~ Po |! ~ om | | | ~ ? : ™N ; : i 9 s 4 qi @ \ 4 a= = W ; ; my ! f al ™ , te ; 7 i a f 9 4 —ae a wy io : ee : va ° ron ae a wf -_ : a ae a : _ a _-+ ae gee 8 4 : | + ; 7 = =~ A mW et or og a oO ea areas = Teeter = areanamod unotnor oad wo : 4 2 - 9 a x 3 2 38 + UW. MICROBIAL GENETICS 19 REF: we 4 oy y i boPNmupwhn=-. oo YVOmM awh = | oP ON OM Pw DH S ey] o ta Loy re “ G ULW, MICROBIAL GENETICS 19 REF: (af 6 CORN DH ew nN = | Brace OW MN MH PF wD & | oo MN MM Pwr HQ eomanrnnpeap pn oa OW ON DYN PWD. e t Sa “UR U.W. MICROBIAL : . | GENETICS bd +E. | SR , May g RE ] 4+ Zz. _ - . 2 _ ~ _— 75 Pancappary , 25 petaton Sou oo OHM NM PWD = oO DN DM WM BW DH & oW ON DOF WD = ouU MN OO bP wD = ou On AM Pw Nn $ pont bin Paget nn Play “ ‘hee aoe. r : 6S. en fe 68° | > 75 °te | ‘e wed, d+), Dey ACL ws w3oey , ‘Avoo ae plato wh my Hite Lac fus/, ond. % an Bist, (2) | whe, | ty bs vente. aa vot | cone U.W. MICROBIAL GENETICS OM ON DOM FWD = ow MWyn OF WT AWD — CODON DOM B WD =: a - ow ON OU PWN = ow ON OM Pw Dr ~ 5 lee f At 1) ¢ Nurnrfe- t rlcowt- ot U.W. MICROBIAL GENETICS 1 2 3 4 5 W 3430 ( | | : _ | OUR ND & Bw DN po PN nw pwn a ( 1 2 _ al all 5 - coe é - 6. Attn © io LS 7 al A YW, 3 4s 3,6 7 Y 9 of Yor 3645 ys 56 48 4 moc _ 1 C Yibwo 304,59 , 8, “3 i,6, : ; d Nideovo 3€ Gh Shi ty wna 6 ° 7 w ( ~ i 8 +o | aaaeet: 43's 9 Oy. af Ab 2 3 4 5| 6 7 8 94 0 UW. MICROBIAL / | GENETICS INTE . 19 c/.3 REF: [47222 1 2 3 4 S 6 | 7 8 9 10 a Bebe | gles eh . 2, fo Pee pom | lee all |S 4 = Cf pte ty afte oi | 4 DM TLB, sm DUB, md! DH sue) |Nbae (EBM Pom) J siti | AOA [rer] T28,* | wll sy. . oo, ae | _ Oo” A oo. : e 5 a. | , : __.. 84 B.. _2s . 20 roe 4 rctipopest : 9 — ¢ . .w bobo © o — te . oll >D wre : ~~ ni ° , _ i] 0 A. be By oO Dyt, wary wey pepclllee , par dole ed 2 RB a A. 4 v rt v _ 3 C O98 ix oO O N 36ro mai ~ _4t . .D wv yyw 22 atts + T, Fey _ - s|| to A. 34 2 __ 2 Il. £ co _ Od 34 Oo NM loo i Ll tee Ragen 8 c og Ne mytly + .- a . 9 ve pe ed - . L t _ Q 307 |A oo 3s | | ot g oo. (9 | 8 ORG 4 bea wd . Af c = La NWO Geel + | al go’ A a ? 4 . B Lo. 20.1 ; s. C og 8 (Me + oma — | os D [Soo | a 6o9 olf + Z . rete De : _ 8| Sol. & o. Le ma) : 9 e ; “ q DO SP yep + Q D 2500 4 WhO r. r Wwe - +. ‘4 | Ge c 1y Oo. e fo 7 2 D L292 pf tes i 3 welt + j . 4l| / 54 } feo + i 6 f . 4 7 Won fm. male am C, : 8 - . ¢ 9 he waa! ad lg tha 4 r ey a | +t, &* - Qo ee . \ _.._. &< power - A - we Lee 4 Foe 2 Due B Gkt? ie eh , 4 2 ie * eyo . . 1 : io - - fol Bl “+ 3 fhe Ay Tepe ily i >. oT ee “ wen ™ fi Ve 4 ne boo da sil ws Ueki ot iC a. i : : b wi | | 8 | | ol | | ry U.W. / . - a! beweriee Weise x W306) ~ Mypampbrrcerf ITC. 26 e 19 SS. . REF: 3 ; 4 5 6 7 8 9 10 Tiarons, 7 Xgl, HIE tonawey TE" 5° pendalits bap eaieas uy Pre 5 7) 4 2 haat regterbd a Deno amet Fel ob in beet gues. se mag . rrrodeecs pprnol aoprsct naght he ) 6 por/nk : “ed peed a 1 wn ' zZ —_ _ . 3 Game | (be. Legs; Pernting t 21 frm). “5 5 P29, | SKP- Dsn,4 \ EA : _tmng Kp MK te M Lac Gar Ma | ty! a -B OOo ~ Io 4A, Sete ? o P',? | 13 ? oh ft _ ib 37 33% 9 1? ° 1 22. 65" a7 ° es a | 30, BS] I. go) ~ 500 Sete ° ° > Go lower O 0 tee 6 «4 2 | a 40 10? oO O| bb ° 1 g He: 8 605 0 O| bet ° pk 7 God 1037 Di Sorte ° ° ° | QO C ob OD Hawn = | oO @NW AH FWD ULW. MICROBIAL GENETICS 19 Moy 14 1958. REF: (<1 8 2 3 4 5 Ul | t i { | | | | | BN i L i CORN Dh hw Dn in! Dom Pp whe. —F ; 7 9 —_— Oo © © CO MN MDM bw De! ; NO fh pw ho @ SP OND MPH DH = | _fmsert Odd | ud t+ Wi “Us! with | TRANS FO OMATIC A ato W 396¢) t p~tsplorts A Cantal, rm T aH fe Ldayg with Feed ULW. oo MICROBIAL GENETICS bes ; fe. 19 tay 1d. ; 1GTE - REF: 1419 1 2 3 4 § 6 7 8 93 10 ' NP recfeelee 2 . i _ tL Gah lien Aepp Washo, IOC Gore re fending 6! per) wit . wZ0 W rot - al rr a . 3 bo Lath { Bl DWP Miv9e 1 w€ - —_— _9 9 / 2 wl prt prety + HOO pop oe A é 104 wl pry froth. 2 ai - i" . MP Miro { wl fale _ No po wth ge the Jotu-_ Sheeky ow B ise pephinfed Sal mS, 8 mk a DWE prow of ole oft flan made | 8 ap 6 _ | ab ani 4 - 5F j ae ; § / To nk zm M/s DMC Ke . < ves ake Gah | Spr (broth dog 7 lw tog ° wok 4 dyn 3 oo 2 | 3 M SF eky owe BH wo ge wee _ . . i ! gh 30fL0 Scamy NV 40 y et Ms t . ay pot i oy i. ye 300% | peeeth Genk pote 3 poo pepe 4 4 5 ‘her bub aon hows of qe. ok no empert Ty me thee on ahout ; ITO) vey Tes Le WiksSo Mm 1.4 auk —2t am 4 why ww all / 9 W3elG m 4+ ae 8S oteg da TL 9 mk . . Ober. fe WW joo om) OS shad =O, Bf Cod) tLe | , oe Blted por frtter Rng 2 : 3 4 ° | : 8 9 i | 0 U.W. MICROBIAL GENETICS -_ U.W. MICROBIAL GENETICS ULW. MICROBIAL GENETICS F Cake Gat Cees ~ Vo tro E> o~ o 10 how bO Cae cemaal™ Cee OM DN DMD MB Ww IN = J & ; T_ 3220 o alg ~~ T _— ? sist _ cuee Po i Jo afse | pape | 8S las - fof 339 ao TO. wfGA | 3G Ce _ - . 4 ~~ bee 1 1 erg | bY f1o% P} \* “ ne i | | | | be he. E ies St . . t a oO oC t i i I i i i i yO he Mo ‘ h. & K ? ea i wo BGS waren DP ON Pw BP wh b L. f £4 18 370 _. Le... 23/ an 3S {Ss iy goo | ajss [raise | age | if iA $ _. —_ ais 44 a L {Xv a ee. tery phedl iy qe L 6 SD S/48 | 3ap8e ] 0D DN pp WwW PP wh ae 2 ~ a SH wee ALL oe ~ SH. 6H TH ee Bib _ StL. 9 amet |. 1 - _ 2th — ae a 3 nee a aos 4 _ - 2 i | bon o T | | | Ryo es fbx po & -efgo | i838 L : ea U.w. a 5 PULSATION 1420, | MICROBIAL dune 11958 REF: 1 2 /3 4 5 6 7 8 93 10 . Kinetic ¢ ting have -unsatisfactory. until now AL. recrui and entry - In Previous 2| contre , varied as approximately 1/2 power 3 of cal e), so t sub t recruitment 4\| aeeour is quite unsatisfactory. a) 6ll In th lich may impede mating wi % preventing 7 further entry. a ae 9]. Folla Of dl. Le pH mM o5 units from 4.5 to 9 Adjust D(Q) by HCl or NaOH Ad 2. tr 2 3. versene a 4. oglycollate 4. Se. 2 s|| 6.dead _6l 7. Lluorouracil deoxyriboside + | not available today 2 8. 9: 58) Fl M/100 = M/1000 M/10.000 8 9. D Z . 9 ORC W-3060 and W-3064 (washed and resuspended] in cold penassay) 0 | minutes iat 37°, Dilute 1:10 into cated splutions (made up d incubate to 60', Dilute 1:100 for! plating 1.05 ml on 118 should gave a baseline count of SRP ludéng some 10% 2ik 3 ox ded ty will be indicated |by a loss of SRP 4iinhibition of entzy will bea indicated by failure of Gal+ among SRP 5 No inhibition will be indicated by lmarked rise in SRP g[Inhibition of mating # inh, of entry will be lindicated| by stabilization of SRP with 7 938 anfeent of Gal+, 8 . g|| Controls needed: | Plate recombinants (plate ture after dilution) ) 0 Barly interruption: plate mating at/5'; ) 121000 dilution at 5'; plate at 5' 15' and 60' ) Dilute inD(>).. _.. ' 1:10 dilution: plate at 5'/15' and 60! ) 2 3 4 5 6 | 8 - al — a Pullin og ‘f pansy Ol pg, Lo ‘ i - 1 - |. oo he . - . apt! S89 na ft, 15 spuatl wdaten pM / ) Bucttuy! 4 tate Miypalint (patel prbiighics avdinell etelind) s é 5 ut GA. 7 8 2. 0 U.W. MICROBIAL GENETICS MICROBIAL Peck poles (p08 GENETICS a ier aD cathe AL AN ane eda A ee Vo U.w. MICROBIAL GENETICS : | a JOO OMN DO We wn = ———t + po | OM DN mw ke wh ee! t — CMUDN OMe wD |! oe OY own awhp = bo PN om ew d= so So Co. 12 8.1¥ 6,9 vd, rY Aw stroked a n 6 ke é ful /49O C fF 20 £ 30 P uo se f go Cc 20 Cc 30e C 4o Cc 60 BR 320 B So B bo - Zac 4t t- g— i+ i 4 it ) 7~ 34 7 7-~,2W T+ » £- é¢, T- “a-— 7 # , io—- (some w?) 6G # : (4-5 fw Zt iy 9- zi +, 34- U.W. MICROBIAL GENETICS Jue 1, 1459, 3 ° |: rr foot i it~ en po | O © DN om jw ie jw | i + + all BL COON DAD Wey nu cop ph mua wpe! fo bos : OO DN mow w bp peer Tw eawp = / is! hth Jo. |e ANB ov V Y ray retofrer fo. vING ceeds : ct Yl Ao cnrewh —» 0, | Oer 7 nr, /¢20 D 8 9 10 as we Sou (0.0% Rie FP hte Df) 8¢ fF bt OS cp. P zu) Wh Way + 1.4) bie ont W320 64 + 0.9 UW. MICROBIAL GENETICS U.wW. MICROBIAL GENETICS ULW. MICROBIAL GENETICS ULW. MICROBIAL GENETICS /Y2A U.W, Periodate MICROBIAL GENETICS #4, we espe cette et EEE. mets EE I ee U.W. MICROBIAL GENETICS Pericdate 1421-B lw ParabeXe, W424e MICROBIAL r U.W. MICROBIAL GENETICS ra lee ay a ME ote EE ae ee U.w. MICROBIAL GENETICS — J une . REF: 1 2 3 4 5 6 7 9 10 ; _ a _ 2 Co unt Ss ccna ch Lo ‘ fer ethyl Chewrea 5 if 3 on 6 _ 7 | 8 /a M9 aU 9 ° . 13 ay ey j 2 eee + 3 S| wa/ ay 4 cee _ Ss cee ; 6 | hey i 7 / 8 _ __ _ a . : an to 9 1é ! - , 8 > Mis 0 _ AL _ 2 oe jO ko DN Im in ip bw lp lo 5 Bo 6 _ - IL 4 - 8 _ — _ _ _ 9 _ hoe oe . Q nao - if - a _ _ 1 4 - | _ 2 — 3 4 pe . _ - — Al 5 _ a 6. ft af. __. - ee BP _ a. Ott _ _ - ——— OW 7 Mw U.W. MICROBIAL GENETICS U.W. MICROBIAL GENETICS _ ~ pte /4r1D beypunrte, o useage res U.W. MICROBIAL GENETICS 13 10 \FFEREWTIAL i Oo} MN im kn la lw ip j= Oh} DN mh Pe wD be OC WRN Om hb ww | Peo my oO ln ie we De ~~ | bog po, pb DN on ew b= n° on VARtouS) ACES fo Agi o WwW toty Le Ab Lead te" Dh ea ts ee ne en Cg de eB Kalb eM ee eh EE a Ds Ce eg ede Be —_ : Mins ia 1 ll, ae 3h te. . U.w. MICROBIAL GENETICS a mw IN i | | oO WON | | : | i : obKD DPN mM mM fF wHN t + __ ‘A 2 all. - a . Al 5 6 Ae BI _ aft 0. 5 po mn Ow la whe oO ON Oh ew De on 302 3 ¥9 387 fom By) wh od ROE Le f . bay iz is? ve ye 4 Heh fo wh bol. - DCI4 &,) STA v4 Yo 3 -0or D, “4 Sas A, 58 Sun 8, U.W. MICROBIAL GENETICS REF: 1423 A 9 10 i | | i Oo DN Db we w iN im T T rt eRe al AL? ait. _ 3] _ _ __ 4 6. woe —.. § . _ 6 ; . _ al dw _ ne ABH on ae DT oll D. 2 we By a 4. . 5 a . AUP 2. . oo BY 9 £. 0. . ! OW DO me wp o ! COON Mn py Dp preted * Cornet) . ‘Ave C90 . woke « C2-&s U.W. MICROBIAL GENETICS 1 ULW. MICROBIAL GENETICS U.W. MICROBIAL 14232 GENETICS The Moris wer alee ted up wut Prunes gtd a de | for AK ard nr tf ~~ oO 1e These 2rateceo Que ; io C wh : awdir trurdhorver SMES ° Damn uth, ad pee i rYo 8 Wo 32 wicnoia. “Les? 14238 , phigic finate ; 238 U.W. MICROBIAL /42-38 GENETICS Lo t ; i t fpemmeecromes nies ene arm ce menecran setae a 5 ¢ i i \ i 5 ‘ “ ' ! 4 ‘ q i : slp ei en ear a me a fee ements me oes ae apa cam \ ! i 2 i ‘ i ‘ j i i : ‘ : ' : ; : i : 5 ; : : i ~ ve ay + } t i i t cen eecene imamate msn os toca nes et semcam ae ‘ ' 5 e { ! ? ' ¢ ! j { } i i i ! i i ‘ i j i : veces we meneame me mmm nee reine sears \ { : i i | { i i ee me ne seme sama me me mcm a mame a ‘ \ i { i jie paren = ances Rea em i ' ‘ i cote nce macs te name onan wee ake (vem a a em a a ge Atm seevmamsenies com enemas comets perme 8 care we meee eta eae Wo \ yr A \ 142A A U.W. MICROBIAL GENETICS U.W. MICROBIAL GENETICS EXE: ULW. MICROBIAL GENETICS 1Om.- G29, [ T Atm: pox j J [401-2 Bek poles 7, L, Y, Lac. baad to _3 P” gettin" atee ww) TL. hte Ts sto ~ honor Conte “4 | ope 306 / v4 FT ae Une. Sepauletey bs tosh wer | cope 1! dP): 12-1 Fae tpl 142 — " /. wuaree Covtrdrtoan? fell. Aug ‘0 es eae sah get pdt box “isl. —> 0 erlrrarla shd 3 Sere 30 19ST rtscon iain Foray pln copa fl 2S We tty far ey Sp ee tod Si ain si aA Bbw tl or perce) ia pris eat Wd OX anece Op sintde wes gn re yal pf tenn he mb 1 Tabet tip firme 0 2 -S | — Lbigld capi 9 pecan prep 7” dof wee, =F" WE. : Z 3 re to phate of Leng oo See aber 2 3/8 yo Petite vag RULE ighlag, > Se YO. Tint : sao pt i eatip ew - . tie Shs Tele ye ; Getiieg, | [Yo fd TP ard 2 a : . i CL Menlo hy yg, 1 ORG eT Paine / led een soloing ead Wd y iplelfel? Gb serial? tbo! “See ash | \ pd loot baawkar eraligte fe scbe. An rakcdny zeal Cope m Lurene 5, prolernacloro (por trrelchors EG ie bet hated g view NGOS -S. (lp ewe | /Yas~ 4 flog tins 6 Aberbrfrauspirerions _ I tte be 7 (stil) le ef g. do W> cliffs bows. pod Aber, yell tt yor = EML “ah ue 108 — | eae _/ a on SO Rr an! f aa b am Loe 4) 13 — ! i 7 i eee td oo ae a // | eee ja. WO-13 th ~ congaant Cire Pals. bead auurmnebren : Pree fut, < Caf, : | Lo Lava Luvin wi CML hanele oF udeweg -% CPT i eptcsk > ( btn ep hg wo rma bt) h pale Cathal hl ita ey ram SAIN St i 5 beet S%, Fat spat > piel rw 3 a6 —> pt Wr r3- Blea be! NS - Md wth fe po -alifeach | No rept dpeets veofiels pews Ve Ate 2, re Ad peesrary tected , b telif ata) * SCY Me yar”, rh wjtrnd, (gor - pact Jp eect pap - Hfeo noe be recreate (ne prdbrrtinn meet rvertirind ). 5 fects Rok SE wwe ‘Naa pe aga : Fe 2-65 wl [Peele J “trorwt sae (417. tb WA helo — poms babeoye any pry cose - LY40 - 260 py ff / 14)7 — wong Ofer Jl. +20! | -2 ~<2o Th ri Ate. bo. GY i4el- S: Thaw sx -¥o 3 re oe ffl rau | In? — wR —% Pang als Aa U9 - | WP cases yo 6 fa la it or -9. pth, Unyli dinacds !) lila, HG. e De sears | Mpesitontivot — a ak Conlin S bole, ly | Leob onlin! r , OA 142 (- fucate J7 Sd (lak ei tal Godt Ske th ba yhtes D.— + ther gciclanl fatths rar yells E. — ROE Wa la ccarhl see /Y2 2 AW gt pen pine iphaad Cos Fosed i re (2.2. ROE + Qt Pegi eteows 1Yr3 Mey shits folly MODE blates Uoulot A, sov/ ode ee PE pg ed | Vdboun é oti . ts alee £2, oH ilag th Fre ~ vei ps fo mont awn W3S5/ x) - lout paddy , (Rpg “foo ver J Seto Mt pu bd fepoouls J May - pus (VE — Thin 4-17, ven Aoalyper of OA Gb died MICROBIAL Piha ag f ial page l GENETICS UW. — MICROBIAL GENETICS Ate. TeP Gl § (426. ¥ t- Dgla: What | 4pel me prs bac i he fs che (Coby bac *), | Aa Gal bee; | tug Crh thee! 2 Bu Gl *foe* 0 phe wa 1th ip Lipo on hop uct st Fen a feed nd bee” da fr (sa lees) AA HK 3 4] rt Lge y */ yy p) 2+ a ot fias (>, 3) ‘ fret on phudt Te toad foe 4 ya. Aap” boc tne ahs dam boet; Coa” hwo ” , Aa bee Col — Bra” bog Oaf ~ fi) (2) (2) Lag nm ta _ 7s ols cpu each fs byledar oe we by mG [4/0 xX Pa alee Lorre teams te st fe ME bi I nn Linke ugned fs [cite Mt! YG iprpe hg yp fi atk Phare fou pusut dylrbe 7 /] F Beyamarn Ltld, sgl Bhaord F3i/. | fea eis FS.F)2 $10, 3,13 £128 benny A fre 24; CR, FAL, FRA F23 27, W Bo, . 21, 24 be fr daeloage F37, 38. Lauf30 0 Letan dn bee FR alert © ese e secede . he ptt baa fe Ne be Aegon fh tick ei Clean, Aun pylor Wu} t raretears Yio ? | | y by D-, Soneret fev bp ™ headlso H plane. * ty Olay)! rc 7/26 PS fo i i ' | i i { | g Sf oF a ~ UlW. MICROBIAL GENETICS ama od anda nil \" 00 ol AVM T AMamMmonmadama Amid | > jo: ae pk Em H | iN | : | i 1 I ' | ¢ soda gi ly, ep ob ro oe peti ei Thi iy FO (Hil ete rer eit Ed levy aye ft ee LY |G ( ee | ro Her 4) | 7 tahignkop gop tibito a alae a hl Baal SHEER AE TSE Ae ee td LO ETS Ee Te ay Wve 4 / 9 fi G é pot f. Ee Z 2a " Y26C! tay Gnas 2 1Yr6T Arg, woth tony aT 08. , Sane a7 f2,0% 10H )OA¥ Aa, (thir rretilts) wb? wyoe wobr Hy, Aer Any Bay JULY 20, 1958. . terminal notes for resumption of work in the fal] 7 - It is now clear that Hfro crosses tend to give both Lac and Lac hemizygotes. It is probably not profitable to attempt to categorize more of the Lac” from the 1410 series; it may be worthwhile doing a time sequence experiment for the pro-, duction of diploids, though it is reasonably clear that the occurrence of Lact is related to the production of Lac’, while the Lac” are hemizygous, in supp ort of the progressive entry hypothesis. But the nono¢currence of GalY (compared to Gal® is enigmatic, and probably some more GalVY or should be looked for However, in orddr to distinguish Gal™ hemizgous from homozygous, it will be necessayy to use complementary Gal mutants, allowing either Gal' or Gal© to be analysed by reversion. This will also afford the opportunity of using Gal/Gal selection for Gal heterozygosity. Therefore the main programs are:. 1. Time sequence on heterozygote isolations, to complete that picture. Include segre- gation of Az, V1 and V6 as now available. 2. Gal x Gal selections of diploids a) are there any Galv; b)Ara2,4 pos.eff. 3. Complementary to 2: crosses of diploid x haploid. 4, Nay be worthwhile to look for automictic derivatives of LaeV diploids as a Lac™ homozygote# would probably be a preferable Q- 5. Time exogenote entry in crosses of Hfr heterogenotes. Probably first item will be the review the accumulated Gal™ stocks for identity and complementarity to Gal,. dn popes poe Mpladbaab Te: hep ? Hfr MICROBhae———_ £° _____ . AOETIES, sfelst ce GH NE V Coagee setts V ond ~ ( west ) Ae yey Jom — _ “tT a ven dp a Peery ) al _ wee ~ arto an ay 42 uy Lm Cand weak oe a raen| -~ oUt - + of TLV - - ; - + - — : - ~ 4 7 eal ta \ : possibly “pev + ae _ — spate UL VOM beh ee Eee pel PR Es ao A ce eatin . . - . — 5 - i tp _ \ ale ssilely po : _ — ~— ° | a4 ll (> a. highly ppt iy from map + re 7 3P |] V — (ard V7) ft — -~ > 8 pe — a 9G 7 ST a = ysl (er Vv? — — ~ + erie | |= osteig lee ¥2) J / O | = sv weak _ — - . u Verde hom me] —Caezti wi] - an ‘ f " . we Per ( alse ta tin: tel) ate _ (nage ol ph AY] Vand) og Kem pop + + = ~ — | Vad ~ | pos hl ov Sen J ~ celen, duce, | — and. | poss hy vam | Besley types U.W. —— MICROBIAL Eso GENETICS o, Dee. 1 199 Sf REF: 1 2 3 4 5 6 7 8 9 10 a eel q any «Lae lhe Are Lae Gah } Ay! 7 - - Bil Vv Lv od Sl (weak) orev | oo ~— AKs t's from ec a ge TT nn pacer ETAT SSE eae - “ee ey . _ 4+ S possibly not —Cehov LM enh PS pele a te t- 4 — but simply OTT eee oH poten ; Ln iktel -> festreadted - . “3 \ee la . ~ _ __ gah te Y. fm) highly Pe " impae + | proved full +t. a 13H ve - - — -~— v - im} v -od vicwek) |yfe, | - = - 4 yo : . { velgably V _ LL _ i” "” = aad Y (weak) Ko ep . a all —_ - ~ — ¢ M 7) ds Y yes "é wW ee agit soy — ee _ 4 _ — - i ry Sra — . mb, z ook: 7 sol voo| —Comdv?) Pe 2 | i _ L 1 - { 7 5 2 34 Vv | — awed Y (weak) v Ser — Lo _ { all yr 3sll VGRJ — ~ b — tes 5 1 lebly Vv é Vv a 7 (wee boda 1 ~ 4. ~~ ~_ bay Se 3 ands v?( CP Eee ep Ves, ? x | _ ley aH! "4 f- | V fer ea fm — - cn Na ol - s “wv i|-/ 5\+ pao: ater Udey 5 tT. t+ ” _ wl ev [ess ppt | ot fe fe __. APPOAL 4A V —_— — _— — — “mtse dl 4 a “pe | . - sl pap Bi treed = - - ~ oe ey : cate . Vs LV LHD sughe ate yds t+ - - V V 4 mi V — dh, pap ee _ _ V — (hee ereelpA col) | — — - ~ ‘ , —— Sen meune -~ 2 - g 5 U.W, MICROBIAL GENETICS REF: EG 9° d CSAP N OD ew iN i 4 sh ep he ft Re ; N“ i oo 2 oy. Wy | . ; 4 7a ae . sit _ sf 77 | 4 4 WBN . _ all 7 9 _ Q oo 1 : 2u a 3 all 5 lt _ 2 _ | - 8 im) _ oll 7 A aa all. ald _ _ sil. 6 7 8 4 oe a = CO PNM MH apne st ¢ [each oy] 4 eel types Pond wear | Cag HA dhe ULW. “| MICROBIAL E So GENETICS = Farents on Sugars U.W. MICROBIAL Peo y GENETICS o-) , 3 4 s 6 7 8 9 10 " Fagillated areas From lac- colonies 3 day s vld were strar ked on 2 3] B® hak +e lool fer V vis a straight tr. 4 5 6 a my i peje) M See next v 9 17 se a? \ i . 0 ‘i é eq | a- i 2508 1 . . 2 -1a Pe? a ere V 3 st 4 hh mene . ins on 2 on fi ria} oe / 5 6 - 3 uf fp 7 why aot? ue 2 f 8 9/8 - V 0 . - /s Vv 1 iF zl - &3 pee Hy VY 3 4 i Sie Mot | ¥ ai / ; , ? - 3 pees ay Vv 8 | | 7 7 - 42 proicply V 9 u i -47 V 1 | 2 “(4 pes wedi, / 3 é 4 -aa S Lo 6 -to pea ely 7) 7 8 -34 V 9 . Ql 36 Pr oy gh Vv 1 -~4i Vv 2 3[9- ; A S - 15 / 6 t 7 -al ) 8} oo? 9 ° 1 UW. (Eso) e MICROBIAL “4 GENETICS Dec. 319 FF REF 1 2 3 4 5 6 7 8 93 10 1 Lrterest centers in Galt Lak- pag lating: 7» SO of fle questionable 2 3 vis | from ses EO A ; Severn) Suspect coldnces were restrenkedl | 4 5 ow Wac. 6 bac 71 8 [- 47 | 9 rr wv { 0 Nenegnaen gi egilinae a emeennanpeneser deacons — 1 PE probably PtP t 1 a-34 “ or t show fF t+ NV P 2 - | 3 u4e- s2 or ? t, 4 5 (o- iT ° v 6 rot , J 7 Gel (5-27? Vv 8 Qi 0 ' Esp Cx Oa Law 2 ye — 3 4 5 y- 17 _ - - + t + ¢ 6 1 ag = - -¢ rot + + BLP ! 2 ve 7| 2 er ey eave On oe 1 2 g- 2 > wt _ — — + t A Z 3 al 4 - + 4 5 , i i x 6 [ Atst beth kinds of Laem) fim Mav J ba Key, \ 7 \ : 8 9 0 . 5-27 Vv = Areal Vv, Ab Vv 1 2 S- t} Vv = ry a \ 4 5 6 7 8 9 o ULW. MICROBIAL , FE so > GENETICS Lac Reactrons of Origine Eso’s. Dec. & wp 5S REF: ee 1 2 3 4 5 6 7 3 3 10 o Streaks on 8 Lac From DM -Gre- B, liquid of origine| 2 31 € Soj's ¢ only fhose circled in | red by J 24 i 4 1 DAY (ane except : \ a DAYS 7 op or PP? 8 /- (4 very few dark > Va V jp orlpinet darks 9 now flopk falf F 9 : a- (34 | al — | bluish e/pinkish |— ‘5 Langer 1 | col gt p> late . Few Fevg 2 im ~brush = area. VES 3 B- Vs , very few kK Baayen bil pop 4 ov iain darks abe look fall ty 5 Lia of west paiilatd Et. 6 ? be § very Sew dack > 4a rer a a (pert bly v7?) 8 ory: darth seem Fault 9 0 s- 5 _— — 1 2 s- op — _ 3 4 i 5 6 7 a} F sO Flom pit & of above, pic\ add streak om Lat and Gra: 9 ae pty 7 "4 or f* . i ' 4 et 06Um {cE tt \ 2 . 3 F 4) Ty pi ing Hr bacl's ot AC * etsy ould tr 5 | F “oe UL Pores xb ; CF Yir 9 le, Z ’ , ~ Q 2 a ] —— at whe & cee Goa yt Cina: or + + pn / ~ Le et we. Pi t 1 oy Meee 54 FO TL Ih P| Lees Xyla Caka Houaa Yr Ve S 3 tu . a" f - oy He all wo be Hir yg Cray | Lage Th, | 4.24 s # 6 rp a aan t —] “ — Hee pe hag 1, Jrfe ~ 32¢ HO fe Sa ee 8 te, 3 0 U.W. oW MDN DO KH &wN = OM ON OW FF WH = oO ON HO PW Hh = MICROBIAL E SOL GENETICS rn 19 REF: 1 2 7 8 9 10 . Free Origine t 4 2™ day Vax. poeiished ) 8 Are dlAcacs 1-4 | early + + + — _ “ Vv nw t, ry V + ) TT) V V, — V , _ — —_ — ie ‘ +t “yen ~_ —_ 2-34 blue v tor + allo remetre — alis vevy Spell ; (- pepe Petey ec C1 Rew y 7 9 enk Vv +, 7) ,¥ ; t clef Wea ty — = blue] - end feu) t ?!! —} © 4 Nev sew tra + ) _ pp" iat 3- 16 Carly Tr tr + _ ~ “ye t, 7, Vv +, Te Vv vo a oo — Wm _ + (alRev). | smal ai Cas aterm) and = Aso |V 7 + yseu. [rev 4o- C early t + tT aa r ve th v nv v Myo ~ — | (1+? probably agcident) _ — ™ y Sdelae pe st a Vv" im this Tan is Ae fi any tel, U J prot psp! 'tatect ow On MO awd — ow ON MMW BW D = U.W. a MICROBIAL 5S of GENETICS Sor | 19 REF: ee 1 2 3 4 5 6 7 8 9 10 woth a doors 4A 4a - a “4 | ME S- S DW Clana UCL pion le Leb flee af mL wy een oy b el pees : 3 frothone. Ow Fad +t Cha ) a, 6 e (fob | oS 4 é ie 5 “ Wrst tek ~ 6 8 U 2 bea . (@) a) +, |V Adak ¢ Fat roe 9 d ax ee 0 - a *. ¥ eee A 4,7) V —, +0 Gt |), ant 1 gre bute € N 2 + rn r Amys we 3 q. + J —) Vv bee | if ath Waters ly ) 1 + . 4 5 6 7 8 9 i 0 ; So 2 Q 3 ~ fo ‘ . i 3 = fee t Hors. C 3 \ feng F Rtg ad Ona : ee ee 6 7 Ae Kae, ! 8 fure \ic _ —_ ‘ 9 “ ° 7 + V } utters ly Dart, pen: lett. 7 4,7,“ ¢ “thee ; af ¢ o 4 7 2 - 3 py. «fl Amik, + 2 ~) Vv yer Abe f A +, -, Wy, hen Het f ey 4 f Ad ' cal 7 8 9 0] 1 2 3 4 5 6 7 8 9 0 ULW. MICROBIAL To “| GENETICS Dex * 19 SS REF: 1 2 3 4 5 6 7 8 9 10 go 81 Wl Freem | so -~r Fier. t Cla | » 2 r 1 3 3 A . Ar ‘ fone tow : (ge. . “+ ) bu Hee -F Vy 4 -~ \ = 4 5 B # gage Une " : ™ + ) J 6 f 7 a a 3 oy Ce 7 peo he (ho @ = - + Conty en ) ? bu Henehy ° 6 | f a ‘ * i co : o D " Oran _ + butiertt 3 ) 7 1 , 2 f a . a aj vem ($0 CAE Gren) ded iP fpr Bak omy ty | 4 ' 2 4 ye | : BB ee Yate 6 ? - | _ (oa 3 8 S Five Aan k d it peak Lraptear « ~ 4s hie Me 9 4 denk. ww 0 4 “4 . Fates for —-, +t, a Vo, 7 dank ' oy 2 3 4 S 4 6 7 8 i 9 ; ° 1 | 2 3 4 5 6 7 8 9 Q ! 2 | 3 4 ' i : : ° ; | 8 | 9 ; 0 i ULW. MICROBIAL GENETICS Dec. 7 19 se REF: - 1 2 3 4 5 6 7 8 9 10 _ 4 241. 3 = ; | Y Dey - 4 Days ‘ Day } a Deys 8 SE Ava B bac 2.8 (Lag fpotate — Ginker ) = t weak Ke weak 3 9 Sr Vv , vG44,-, vo] v VIG, dack + lt any ) a ) . -'s s Int ire 2 WO pnet 4 g | S-S7R fron ~¢ egy yt) | -| ° a 1 Ota flee t Tron ) + - - 3 2 Vv 7 Vv v (#| -and Nghrthish v) 3 ne pot + Cc fal Se S74 Fn ad = - - (segregating 2) 7s Lac poe - - t +r | 3 6 Vv v v vV(z:ve-h _ Zz - co rd put ? Db ‘ah S-S7e fron - ~ - _ 2 a mk plate v V (donk ¢ af v right CV (“pal U5 me poet: 3 OT} Vv Vv (vo e +) iY } Vv ae es " ez | S87 dake capital - - 7 = 2 2 _Leeom Ore. aad + + 33 “and ligt Srna! V V Y Che prre. t+, —‘s _ 4 a (ve light SCE rts atlog F og £-5 1 dark pagilleted) - - Td - - oo ) ? sll Sera bee. V v Vv V (we gure -' 2\ 3 7 4 Vv Vv V V yio er< te 8 v v (7's seqven qiting V wots . 9 . © 1 ofS STdmkh yf Smad - - - _ 2 Crt pits |v V (Lett dak iv | v2 | segregating @ + types ? 3 _ 7 —ad® jight Y) - VO- = igt v) t w2 Vv. V Cig & dar v) v No~ 54) tar 2 . a H._) alge 57 dake _ —_ (rererte | — + 25 Ser | Lac a v V co ‘ v 2.6 ” + ouely vastt lad readi—y ) 4 - Seqveqat xy & 7 V Vo, poe t va ty es v> 8 . 9 _ o|| Mote Yo means all) 3 ty pds Ct 6, ver iqatedl ) aniess jotherwise . ————] _ ‘ we teal . So Dal / (cla vdet pit cert) a _ 2H. KH 4 Dota eck “p ay t dart Seutes Lok) streant ed bac t Ara 3 F 4 nee _ dette * wag’ us . “ 4 G 2 Drie tet y a aie. oA Vv r~ Fg. . sil 2 3 Que “te awh j= ote [Lae bk ne 6 | E 3 bles pee PEE Onde 8 Cl Le fer " : ol Ag cd. + ahde Ae v2 Rey he oll. von - | Oo GENETICS Fo. Dec. 1219 5° REF: ys 1 2 3 4 5 6 7 8 9 10 woke A ao GH amd | Bae / 4 oe | B- Che e4tedld Ae pretlne 5 Zam ad AS. Aig hte, (wheker ) er eg 5 ohh, aot af tf. y él . a. z Mepsccrtatinn F tak, | arene, Aeatric fad ory Raw bad Ca all | ol te abe qe the Kew stathinw ab fev andl fo au Y Ke 0 _ ce ent. Le Ac L oll | wee ges ot ple Cf procteceel BIL 3 4 sll él. lf _8 oo) _ Oo . Bll. 3 all. 5 6 _ _ 7 3-46 b as 8 OF _ wer pte é Re ~ ! ol 2 Chee 4, WH | 0 2 } at Ae pti wlhi~ — . & | Y — ld + op tlate — Add Sj — can f ws 2 2g. ~ all 7 a a A £ ~ + Sl. 7: _ 6 1? — 7 “ ~~ 8 cen ef oe fe VY —_ 9. - ol 2a bt | forge tn - L 3 i _ 2 ee & - 4 o- 34 ore / _ Sh Ail ~~ all 3 > 1 ¥ our td L803 fick pfs te ST “I HFA rdistrene ake UW. MICROBIAL s“o Tr GENETICS | Dec. i219 £8 REF: _ 1 2 3 4 5 6 7 8 9 10 al Ara bn hae). typing of siugle colnies ftom sb ED. fll prekef _. A voce fe . 3 Fram |. Ora . A ne _ 5 noe a GI EM -Ara- |B, Ts Em- Lac 7 » 4 _ 2 Lt . 8 WHi63 Iwyoge |wHoe? wid lwrPss fw 43 5P _ ol _ B-tk. large, non - +. + _ 4 ook ~ - _ ll | _ RS - + + + 3 eek - - _ Ad " _ t+ t - - a 7 2 “ al + + + ~ 7 - 3 " - t + a ~ } cre - _ 4d J “ _ + + '¢ + - ~ - 5 - ~— + + & y — _ - - 6| ‘ - + + £] + | ek - - 7 a ” + + Y ~ - - 7 _ aon BI " _ T + s t _ _ —_ _9iL Lacpa —_ + 4+ 3 ~ - ” oO ep - + + 1 r ~ ~ - . 4 _- v + ~ +4 _ ~_ _ a Af . 4 ” + T Ss + - 7 a 2 late ot ; ae _ + Wa + _ “ _ _.. all ob le Stall {os gep) + _ + > 3 ok - - - 4 ” t - + = 6 cok 7 - S|) a + - + 7 - - — 6 m) + _ 4+ — | cok i ek _ 2 “ + - 4+ — 1 tg = - _ BL. CO + - + — J tok ~ _ 9 Law + _ t+ —_— { cok ~ — Of i + _ t tT - - - - ce ~ + ee ! | a-d4)] I - + + — | - - 2 on oe - + + ~ ra LE ok - 3 “ ~ t + oa - _ ~ A " - + + 7 4 —_ i _.. Sl steal +r - + vf 7 + sect] Seok | Seu ene) 6 we + _ #sc ~ + seu. cd Lt wl ees 7 ‘ue + _ + t ; —_ _ ao - 8 > t 7 +f — po tek — — 91 , ed _ oll. al. x 4| - _ .2i | ai. 4 ce Sti. 6 ; dl | | 29 7 a \ q- 3X Ip ioe oO MON MO OB Ww us 4 iD © ONO HW Rw Dr = OU PN OAM Pw DE rt hu \ | vii U.w. MICROBIAL 5° K | | GENETICS nes 19 REF: 1 2 8 9 10 il Stveakk vem! o kp of FOLD. 3 en. % hac 4 5 . cn ad , have 4 bead! _8| 2=|ht ae (Ad oid) ZA _ a 1. tL a wn all. Sr 7 dt ? Py t 29. - ay Q 47°49 : A th mate Ah 2 ; 3. Re strek ow 4] 5 6 GB Lag 7 Ff al. oy T 2 N\\ OE & ee é weed, ULW. : . MICROBIAL Po Z GENETICS — 7 ; Dec. 12 19 58 _ REF: _ { 2 3 4 5 6 7 8 9 10 _ 1] Testing. ef bee test! system. __ 42 . 3h. _ 4 2 ao 6 M bac. Meth - | M Lae (Bi was a TI 8 gaat A736) 43s? | 3229 3956 of - 0 wa 7 OL oe rt |]¢ — +f — + + a | prototragh ) mt) a A + ewes ' - 7 _ 2S OF be 9(Rec. Syn re growl ty eltes, 5 be . i t re " ~¢ 3 and 38307 | Brow vg Lande wall * yy palsor b # 7 (foc = nett . +> + =f overly oy r G aK \ : we Mle HAS (Rec. ¢ SPM — r= Y vee ly Co px 7h. ad wt lo sdareS | / _ 9 —_— _ ae A alls-4 # 40 as abevell ~ T yo Be #2 — -. a O bet | linst tad of dene 0 3] ef. Brewin ogy _ 4 | wale . 3 4l 5 _ 6 OL _ BL. 9 _ 0 “| 2 3 . 4) Si. - 6 . 7 Theoret icall Ww: BY 7 . . al 1 6 352 0 Ww 2271 w3236 | wa lp ~ - +t w 3adb tte hem, iL oe _ wads hfe mM 3 + + 4 baciy3. “al - Jj — cant WH ESP Ht Lac’ (ras _ 5 aa boo Th _ 6 T —_ + . ~ won EY. Ab 7 . 8 : | a a Qu ULW. MICROBIAL ESO M GENETICS Lo . £2ff2_19 FP a 1 2 3 4 6 7 9 10 7 Up O Oe dg Efe gl - 2 i a| EM- fira- By EM- bade didut yu. ft 4 4ios| ucce ese, saar, * SB W_ date | W igecapeg YW Bale | WS 6h yn te LH + - - - 7 Lo @ ot — - - a Bi. is! r - - - - ol. w - _ 4 _ - OIL. _ S Araltr i - _ . . £0. - T ” . - —T4 dey @ oe | + - 7 _ 2 zr _ + 7 ~ _ 3 & | - + = - _4 4? - t 7 ~ 5 7 ~ + - - _ _ 6. t2 —_ t - - ae : 3 | Gag +- ~ + (ik) - Bll. ly - + - - a 9h AS. _ + ~ - Oj} fb ~ tt - - . othe i7 — + - - Ad Pd i + - - 2 @ + _ - - ..3 ~ + - - _4ll + — - ~ Sif + _ - - 6 Cale + - + (sco8 2 _ Tt - - _ _. B 7 tt ” 7 : 9 - + ~ - 0 _ t - - Ob t —_ 7 | 1 ~ + —_ 7 ' on all t _ i - ai, - _ t+ - - ; - 4 QOAe + — ~ _ Si. - + _ _— : 6|| tT - — _ 7 + _ _ oe 8 _ + —_ — _~ 9| + - _ — cones en Ol f ~ — — dL t ~ 4 (ook) 1+ Cy of) oe AL. — t _ _ 2 ~ + ~ ~ all + ” —- ~ we 4 ~ + - _ 2 5. ~ t _ - 6 _ 7 8 - 3 QO 10 > Ts «4 Ge al wgke Ae * i v a oyu pe & : {ss ee if "kh nu REF: w373¢ EM- bac dadet gon w3224 V x ee 4, Phim ae beri, EM- fira- B, Hla pe ee tt Vay -4 evant vee V ACs. SLR I pri te | hee yy | 12/)2 9 FF UW. MICROBIAL GENETICS @ Qj wv ~@ es! aEethO™ Se ASS Xs vs ree ra) AIORO DADS 23 | q ae : . r r + Te yo i | a Pe i ho@ao AG @HGneoao NM THOR Gao Aa Tw OPODOaG “Ao ew ah ULW. MICROBIAL GENETICS 10 © 5 iL +~* oe >} =~ 7a Sos ‘ Ea sy 3 z 3 = = of = SS K al Perr babyy vl Vay yyy blow, VVTVY Ve path ayy £, © We | | Pyub Peavy dad dea py vba be epee pd dat ebb aa . -thvilba blir, rd se oO rev ir ae a ee oe oe ol g x a . | x é Teese ter eather er 1 ter b bd bir db ypy titel the gay t ! ol % | ) a i = ~ ! ~ | % ho ~ “sa MT MH ONDA ~AO ew oN @a0 “ae wor gad “Na Fw ON a oO ULW. MICROBIAL GENETICS REF: | | | | | | | | no bop Po OO ® NO [on lew i lo st == : all ~~» st? er oe 7 AE 8 Le 9 47 0 In i | = OO PNM WO Be w atT BD | | i i io ONO Mp of WIN + 2 _ BF BF. _.-&e ob PUD Hew Dn oH _. &A 47 3. | 3 pt | 37 vy re) tel ppt a | Pet ttaa sd i & tet n te roppp FF C t : : TTS Le pa \* \ LAe-8, | . +heoa ttey ya ttrag tt + + em 3224 L n- Lac SZ3¢ dnt Rie” bs fee. ae 10 REF: MW 4 te gt ataae rat teh Vetere ge te eer rel 44 \ : : : ; ! 19 Oe Ba y —_ vi Miarigee Sani, 1 Vio a) (2 UW. MICROBIAL GENETICS t pp — + eMeud se gQae OS = Roe | AY 3 | ee iy i too. i i : 5 6A : po : = : ay ; ' i PM BK & ‘ : fo F Po | hog a ! q won a awa -“|N Om ae nm oO MN a om Oo iA a et wl oN a a oO TN OO i | oy i i toy : | on : it i : : i i mY i i | ! { i i toy : i 10 iE Som REF: ues : Sm, aia ph) Ay Re are trary r ¢ Perv edbgadia ih bay 19 U.W. 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