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Woke. PIXP2 PlrR) PH ¥2IR) Poe. / & 7 lac? l~ 077 A, wrosrx w/s2t EMrelen. — MAIGRHKS eacad. 7 O 4 pac. Be EV flac seen. IT STA yh nl baer ZIGNH- FA+E. 078 A W895 x,WI9E EMblac. Tet ntMrtl 3 7 SS bact/. bactl- Yb raMMB hod Mal+ @44) #9 bade PUR LactMaly SX @ bac —-Muls 8© bec, MalS tetfmh. (Addl. Kecaorl fips MDL Sor NA She Ya Hel niechnf 2) BB. W20S7 xW)32] | WW / 1 det /- lac, Gaff, S. C. W057 ¥ W233 2 z 4 lac /~ Hak KAM S con condanct” 1079 A. WIS x%wW2333, fest bac */- fr Sh: Pp wy 2{YAr x w2333_ Maer 2 (14/2) 7 pan, SO fact 2 Myke biared Same RV yg bt be xcordany jor ae rane, au codons meijscrudl ao rit ume-call crigiee . Be. (MR cruere ret pled!) (192) 2 0 10 Cabth dylt Cl .rctline? 7 SOS ¢ apna he ]5%. ca Mio tf Pekiriinnaey Haak A Pree ysrerny Arogebhs ~ rece Tarteg Rereye Te,” Hcl Dlr yet B eee adhe dell, - Thun ee Ayre red Le rer beta | bdewihy oh. Manta eevee athng pore tipper, mel, eet lin tae Moor patel fandudvhe, me dipisa Q. edge pip Mareref Ormbs nicke pherl poitref io -auucerre, (billing CE Irondes Nau be te poce tated ? bh Ss > _ ~.s 40 50 = = mn Re om 2 \ oR e_ ( Le > < uo- /0 %o Wu -33d33-— 8 ) WI2Zsrn po 2089 (uy SY) rep ten, -soraBele unset rete ouch ST Mal) oath t Lawrwtbh' \> SPA. WISP oy. stellt (he) 14420 Ibo hase loa/ if — W258 = wg VF = MTEC JS 00 eer | mace matey a Cael fe ccdle t Maite fd opt ipo W)20A > sat oa Rernrevsh by EYL forencE vf Brfn f/f” es pa cloghes 5 Mitints hare nvula, GfAaT (05 WOT Loo nn vat ae oe i Wala aiatly pecs aul be = ertoe C SM 13 an Aout, yrcte (Blake poner 60), cedbt, slick Neth te SO eeatere. Occ. poe laca $* = 8/6. Mt bee OM ja + prow (s) A + — KR RK a) + o K RR ¢ wait rt: GF RR wh | - t () ve 2338, Yo 4b | / : TF | - Wed ve wee " = Wet G OY ® oat ee Si te i) \- + Oo oe ~ C3) c : [3 4 © 2 ht pa « f- he ot g. “YI? 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Se Ly ! is x ® AT con Yow BY Com penta te of toke Oe nre2_ Spek & Liat lavs s? eR (optus 6 #7 / 39 bs 77 1 476 A3 8 30 2 707 72 Ss Sh | wary WR 19S GR 8 1G TE i 9 ‘CO ep ig. pace Mo pace f 7/5 reutliar, Fon Yat Were tutte H Ponty praks TT con tinnsl meso by acon | mee field | mo iabh at plete! on les* + pens §° Tp , lee “Ant oot | (T= tote wi9 sb) “Meloveng dS | 7 3 B 13) 5% Ae it- ¢ [eMBLr Sy 2 ! 3) H * 7 /y tise t/s if 39 " ] oF 7/7 HF / sce 2 op g 36 ” 0 2 ah 7/s Wise, */ | 8 at 7 i 23 totaly ' 2,07 §52 | wD /2 Ser | | an atobls lh) platted on! A plea : , bac lar ~ \ lag t ubpied.. - T/ie 1% Sb 3/; 4 SAG popr~ Ton), 2 R T/A if s/y. > § apron plate | | PUB lac. 5S | | Q “19 ahs a i! Cat B fae it ie t | ? fo. i * PK tbbue Tz, 3/5 2. " 2 0 6 Y fry ee tae Th V1 7 " g / * —— (est Lia : as tel L teh 71 2 ft 5, + drohoe| 7 f (2. st 12 a eee fe tate. 3 eb #0 , | § 5 a, UNIVERSITY OF ILLINOIS DEPARTMENT OF BACTERIOLOGY 362 NOYES LABORATORY OF CHEMISTRY URBANA Now. 24, 1953 My dear Dr. Lederberg, Did you really think I would remember? I'm afraid I can't tell any more about the experiments than what is recorded, which isn't much; ig it? This is the best I can do by way of sum ary- in I do remember that/the filter paper transfer experiments, before I started using fz and selecting marked (Lac-) cells, I was plagued by a persistent excess of Lac # and/or 5® cells, those which started to grow under direct observation but failed to produce colonies on sm agar. Several times I assayed the parental méxture to affirm that this excess was greater than might be expected froma «igher biter of W-1895. I also tried inoculating fresi: broth fpom the mixture at the time the cells were deposited in the micro-champer and reassaying at the time the microcolonies were plated, but something always hanpened to make these assays unreliable, and I don't know what happens vo she proportion of the mixture in broth. Do you? The single cell isolations, 7715 -7/29/32, seen to have been plated on Lac without sm. I recortted that the Lac fcolonies from the mixed plate, 7/24 were tested and found to be $Y. Probably those from the two mixed plates, 7/15, were also tested and found to be g®, I don't lmow about the Lac-. It could have been the result you suggest, but I wouldn't base any conclusions on st. I think I saved the cultures, but if you can't find them I don't suppose I could. the Lac #plate in that (7/15) experiment probably arose from an unmarked cell that stuck to the needle and got pulled out by mistake (see drawing of isolation). Good Luck @ amd Happy fankszivéng so you and ssther and Seymour. oh Conclusions (11/28/53) oy parent Weg betys (re) Ethelyn's experiments were directed at a different objec tive. A few cases of cells giving SR+ recombinants are recorded. Unfortunately most of the exper ime nts involved plating directly to FMB Lac sm. Part of series III was plated on EMB Lac. There were two occasions of Lac+/— from l-cells. But the two from 7/15 (presumably sisters) were not saved and there is no explicit record of tests for S. ERL thinks they were both LactS$/Lac-s* (parentals). 7/24 were saved, presumably Pl + Rl, are being checked now. Her work is therefore not too useful. [My recollection agrees with ERL on the 7/15 expt.]. Lotinn. ann “fess +t. Annas Ick each Lear Joes sha A. 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