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3-20-53. Motilize abony 1 and‘2. Prepare FA 140 and 15C resp. from single colonies.

Plating of lysate before heating showed: 14C 24.b:
bs:

15C 0
These FA should behave substantially pure.

l enx colonies
20 enx
Prepare suspensions of TM2 and SW950, phases i and 1,2 from single colonies.

Plate mixtures with FA on i:12 serum SS agar,

A. SW 950 (4) + TM2 (1,2) x— 146 [brenx] -l gl 1:1 culture mix + 4m .2ml PA

9P26 pipette spread.
B. i- 12+ X— 150 bsenmx
10A27: All plates rather overspreac
C. SW 950 (12-) + TM2 (i+) x-- 140 (medium still tho moist; insuff.
antiserum? )
D. x— 15C
Pick whateyer swarms as possible, and stationary growth (A,; Bo---). Streak these out
as well as inocula.
gross slide agg. Colonies on EMB Gal Individual colonies
AB inoc i++ 12++ -=+ 12 7" all 4 all ok/ —
CD inoe w " -=+ alt i all 12
Ao £4 12++ ca 5+:]- all 12 all i ok
Bo + ++ 3+s1- £5, UME ye) "
Co ++ 44 +s. 5 i:hd Lik@@2) all i:12 ?
Do ++ ++ +2u 5 i(12) all i:12 ! ?

A30 .

, . Co and Do reacted very poorly directly from coldnies and were therefore
reinoculated into broth and then tested. It is still mystaious tmt they should show
this diphasicity. Restreak and cf. C-D inoculum.

In first run, A and C gave discrete swarms; B and D were badly overspread, and must
be regarded as pooled (and possibly biassed)
swarms.
A: 1-5 all Gal- b These are in agreement with result of 979JK,
G: 1-3 | Gal+ b and may also show directive preference of
recipient phase (homophasic)
B: 1 Pred. Gal~ (5/5 b); few + (5/5 enx) Count 1 b- 1 enxt+
2 almost pure Gal+ |. 5/5 enx ove "
3 pred,. Gal + (5/5 enx);few — (5/5 b) " "
4 pred. Gal+ (2/5 enx 3/5 12%) - 5/5 b a "
3b- 4 enxt
D: 1-2 All virtually pure Gal+ b. 3 b+ seeee
P28 Rerun §,D using smaller incotha (same suspensions). Still overspread, but mod. well

isolated swarms.

B: 2Gal-b: 2 Gal- enx [sic]
Ds ll Gal+ b : 2 Gal+ enx : 1 Gal- enx
Total homoph b heteroph b homo enx heteroph enx
A a5 = = =
B Ss ¥ BY aaa
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